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AB229423

Human Apolipoprotein AII ELISA Kit, Fluorescent

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(2 Publications)

Human Apolipoprotein AII ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein AII with a sensitivity of 7.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

Apolipoprotein A-II, Apo-AII, ApoA-II, Apolipoprotein A2, APOA2

3 Images
Sandwich ELISA - Human Apolipoprotein AII ELISA Kit, Fluorescent (AB229423)
  • sELISA

Lab

Sandwich ELISA - Human Apolipoprotein AII ELISA Kit, Fluorescent (AB229423)

Example of human Apolipoprotein AII (ApoA2) standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Apolipoprotein AII ELISA Kit, Fluorescent (AB229423)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein AII ELISA Kit, Fluorescent (AB229423)

ApoA2 concentrations in 10 individual human serum donors. 100,000 X diluted serum samples from 10 apparently healthy male donors were measured using this kit.

Interpolated data values corrected for sample dilution are graphed in μg of ApoA2 per mL of serum (mean +/- SD, n=3). The mean of ApoA2 concentration of these ten individual serum samples was determined to be 209 μg/mL with a range of 143 – 225 μg/mL.

Sandwich ELISA - Human Apolipoprotein AII ELISA Kit, Fluorescent (AB229423)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein AII ELISA Kit, Fluorescent (AB229423)

Titration of human plasma-EDTA within the working range of the assay.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Fluorescent

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.2 pg/mL

Range

7.8 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Apolipoprotein AII (ApoA2) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AII (ApoA2) protein in human serum, plasma, urine, and cell culture supernatant samples.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Apolipoprotein AII may stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. ApoA2 forms a disulfide-linked homodimer. Apolipoprotein AII also forms a disulfide-linked heterodimer with APOD and interacts with HCV core protein, APOA1BP and NDRG1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" }, { "reproducibilityType": "Intra", "sample": "Standard", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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Recovery

[ { "sample": "Urine", "range": "89.3 - 90.8 %", "average": "= 90.2" }, { "sample": "Cell culture media", "range": "122.2 - 127.8 %", "average": "= 125.2" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-II (also known as ApoA-II or Apolipoprotein A2) is a pivotal protein component of high-density lipoproteins (HDL) in the plasma. It is encoded by the APOA2 gene in humans and displays a molecular mass of approximately 17.4 kDa. Apolipoprotein A-II is primarily expressed in the liver and secreted into the bloodstream where it plays a direct role in lipid metabolism. Its structural composition includes a highly amphipathic α-helical structure that facilitates interaction with lipids aiding in lipoprotein formation and stability.
Biological function summary

Apolipoprotein A-II serves as a significant regulator of HDL metabolism and lipid transport. It is an integral part of HDL particles contributing to their stability and function. Apolipoprotein A-II influences lecithin-cholesterol acyltransferase (LCAT) activity which affects cholesterol esterification. Although less studied than its counterpart Apolipoprotein A-I ApoA-II's interaction with lipids and proteins such as CETP (cholesteryl ester transfer protein) suggests its involvement in lipid exchange and reverse cholesterol transport processes.

Pathways

Apolipoprotein A-II impacts the reverse cholesterol transport pathway and lipid metabolic processes. ApoA-II works closely with its relative Apolipoprotein A-I both part of the same HDL complex. Together they play essential roles in maintaining cholesterol homeostasis and promoting cholesterol efflux from cells back to the liver. The mechanism by which it modulates the function of lipoprotein lipase and hepatic lipase further implicates its role in lipid metabolism and energy balance processes.

Dysfunction or abnormal levels of Apolipoprotein A-II have associations with cardiovascular diseases and metabolic syndromes. Altered ApoA-II levels correlate with an increased risk of atherosclerosis as its imbalance can drive perturbations in HDL composition and function. Moreover research links Apolipoprotein A-II with insulin resistance linking it with ApoA-I imbalanced lipid transport and metabolism in obesity and diabetes contexts. Understanding these connections may guide therapeutic strategies targeting ApoA-II to mitigate the risks and progression of these disorders.
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Product protocols

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Target data

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
See full target information APOA2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neuro-oncology 25:1123-1131 PubMed36472389

2022

Association of circulating markers with cognitive decline after radiation therapy for brain metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Huntoon,S Keith Anderson,Karla V Ballman,Erin Twohy,Katharine Dooley,Wen Jiang,Yi An,Jing Li,Christina von Roemeling,Yaqing Qie,Owen A Ross,Jane H Cerhan,Anthony C Whitton,Jeffrey N Greenspoon,Ian F Parney,Jonathan B Ashman,Jean-Paul Bahary,Constantinos Hadjipanayis,James J Urbanic,Elana Farace,Deepak Khuntia,Nadia N Laack,Paul D Brown,David Roberge,Betty Y S Kim

Antioxidants (Basel, Switzerland) 9: PubMed32967340

2020

Paraoxonase 1 Phenotype and Protein N-Homocysteinylation in Patients with Rheumatoid Arthritis: Implications for Cardiovascular Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jolanta Parada-Turska,Grażyna Wójcicka,Jerzy Beltowski
View all publications
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Product promise

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