Human Apolipoprotein B ELISA Kit (APOB) is a sandwich ELISA designed to quantify Human Apolipoprotein B (APOB) with a sensitivity of 1.8 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 3.906 - 250 ng/mL
- Cited in over 10 publications
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Publications
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- Alzheimer's research & therapy 14:1942022ApoJ/Clusterin concentrations are determinants of cerebrospinal fluid cholesterol efflux capacity and reduced levels are associated with Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-An Ko,Jeffrey T Billheimer,Nicholas N Lyssenko,Alexandra Kueider-Paisley,David A Wolk,Steven E Arnold,Yuk Yee Leung,Leslie M Shaw,John Q Trojanowski,Rima F Kaddurah-Daouk,Mitchel A Kling,Daniel J RaderPubMed 36572909
- Frontiers in neurology 13:8860402022Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMailing Huang,Zeyu Ding,Wensheng Li,Weibi Chen,Yadong Du,Hongyan Jia,Qi Sun,Boping Du,Rongrong Wei,Aiying Xing,Qi Li,Naihui Chu,Liping PanPubMed 36003300
- BMC cancer 21:3152021Detection of tumor-derived extracellular vesicles in plasma from patients with solid cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia R Vitale,Jean A Helmijr,Marjolein Gerritsen,Hicret Coban,Lisanne F van Dessel,Nick Beije,Michelle van der Vlugt-Daane,Paolo Vigneri,Anieta M Sieuwerts,Natasja Dits,Martin E van Royen,Guido Jenster,Stefan Sleijfer,Martijn Lolkema,John W M Martens,Maurice P H M JansenPubMed 33761899
- Journal of Alzheimer's disease : JAD 74:563-5782020Correlates and Predictors of Cerebrospinal Fluid Cholesterol Efflux Capacity from Neural Cells, a Family of Biomarkers for Cholesterol Epidemiology in Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEleonora Cipollari,Hannah J Szapary,Antonino Picataggi,Jeffrey T Billheimer,Catherine A Lyssenko,Gui-Shuang Ying,Leslie M Shaw,Mitchel A Kling,Rima Kaddurah-Daouk,Daniel J Rader,Domenico Praticò,Nicholas N LyssenkoPubMed 32065798
- Cell metabolism 29:475-487.e72018Interleukin-17 Drives Interstitial Entrapment of Tissue Lipoproteins in Experimental Psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Hao Huang,Bernd H Zinselmeyer,Chih-Hao Chang,Brian T Saunders,Andrew Elvington,Osamu Baba,Thomas J Broekelmann,Lina Qi,Joseph S Rueve,Melody A Swartz,Brian S Kim,Robert P Mecham,Helge Wiig,Michael J Thomas,Mary G Sorci-Thomas,Gwendalyn J RandolphPubMed 30415924
- Journal of lipid research 59:1927-19392018Directional ABCA1-mediated cholesterol efflux and apoB-lipoprotein secretion in the retinal pigment epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas N Lyssenko et. al.PubMed 30076206
- Experimental cell research 361:333-3412017Serum starvation of ARPE-19 changes the cellular distribution of cholesterol and Fibulin3 in patterns reminiscent of age-related macular degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDinusha Rajapakse et. al.PubMed 29097185
- Antiviral research 148:5-142017Avasimibe: A novel hepatitis C virus inhibitor that targets the assembly of infectious viral particles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLongbo Hu et. al.PubMed 29074218
- Cell reports 21:431-4412017Differential Regulation of Lipoprotein and Hepatitis C Virus Secretion by Rab1b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesConstantin N Takacs,Ursula Andreo,Viet Loan Dao Thi,Xianfang Wu,Caroline E Gleason,Michelle S Itano,Gabriella S Spitz-Becker,Rachel L Belote,Brenna R Hedin,Margaret A Scull,Charles M Rice,Sanford M SimonPubMed 29020629
- Toxicological sciences : an official journal of the Society of Toxicology 159:159-1692017Editor's Highlight: Mechanistic Toxicity Tests Based on an Adverse Outcome Pathway Network for Hepatic Steatosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle M Angrish,Charlene A McQueen,Elaine Cohen-Hubal,Maribel Bruno,Yue Ge,Brian N ChorleyPubMed 28903485
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.906 - 250 ng/mL
- Assay time
- 4h
- Sensitivity
- = 1.8 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Alternative names
Apolipoprotein B-100, Apo B-100, APOB
What's included?
Recommended products
Human Apolipoprotein B ELISA Kit (APOB) is a sandwich ELISA designed to quantify Human Apolipoprotein B (APOB) with a sensitivity of 1.8 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 3.906 - 250 ng/mL
- Cited in over 10 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.906 - 250 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 1.8 ng/mL
- General recovery
- 94%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 5.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Apolipoprotein B (APOB) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein B concentrations in Human plasma, serum, CSF and cell culture samples.
An Apolipoprotein B specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein B specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein B captured in plate.
Get results in 90 minutes with Human Apolipoprotein B ELISA Kit (Human Apolipoprotein B ELISA Kit ab190806) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.
- Biological function summary
ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.
- Pathways
ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.
- Associated diseases and disorders
ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.
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2 product images
Sandwich ELISA - Human Apolipoprotein B ELISA Kit (APOB) (ab108807)
Apolipoprotein B measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
Sandwich ELISA - Human Apolipoprotein B ELISA Kit (APOB) (ab108807)
Representative Standard Curve using ab108807
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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