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AB320791

Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular

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Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein CI/Apo-CI with a sensitivity of 14.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Apolipoprotein C-I, Apo-CI, ApoC-I, Apolipoprotein C1, APOC1

6 Images
Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)

Interpolated concentrations of Apolipoprotein CI/Apo-CI in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Apolipoprotein CI/Apo-CI concentration was determined to be 179.9 µg/mL with a range of 98.2 – 282.7 µg/mL.

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)

Interpolated concentrations of human Apolipoprotein CI/Apo-CI in HepG2 cell culture supernatant. Interpolated concentration of native Apolipoprotein CI/Apo-CI was measured in duplicate at different sample concentrations. Undiluted samples are 6% HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)

Interpolated concentrations of human Apolipoprotein CI/Apo-CI in serum in 96-well vs. 384-well plates. Interpolated concentration of native Apolipoprotein CI/Apo-CI was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 100,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)

Interpolated concentrations of human Apolipoprotein CI/Apo-CI in serum, plasma – citrate, plasma – EDTA, and plasma – heparin. Interpolated concentration of native Apolipoprotein CI/Apo-CI was measured in duplicate at different sample concentrations. Undiluted samples are serum 1 : 100,000, plasma – citrate 1 : 100,000, plasma – EDTA 1 : 100,000, and plasma – heparin 1 : 80,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)

Example of human Apolipoprotein CI/Apo-CI standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human Apolipoprotein CI/Apo-CI standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)

Example of human Apolipoprotein CI/Apo-CI standard curve in Sample Diluent NS. Example of human Apolipoprotein CI/Apo-CI standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14.44 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Apolipoprotein CI/Apo-CI SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein CI/Apo-CI protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human Apolipoprotein CI/Apo-CI with 14.44 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 102 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "96 - 118 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "96 - 106 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "95 - 107 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "89 - 96 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein CI (Apo-CI) plays a mechanical role in lipid metabolism where it acts as an inhibitor of lipoprotein lipase and hepatic lipase hindering lipolytic activity. It is also known as 'apo 250' and 'apo atv20' with a molecular mass of approximately 6.6 kDa. Apo-CI is primarily expressed in the liver and secreted into the plasma where it associates with high-density lipoproteins (HDL) and very low-density lipoproteins (VLDL).
Biological function summary

Apo-CI influences plasma triglyceride levels and cholesterol homeostasis. It plays a role in lipid transport and metabolism by modulating the binding of other apolipoproteins such as apo A-IV and interfering with the binding of lipoproteins to their receptors. Apo-CI is part of a complex of apolipoproteins that regulate lipid metabolism working in tandem to balance lipid and cholesterol levels in the body.

Pathways

Apo-CI is actively involved in the lipoprotein metabolism pathway and cholesterol efflux pathway. These pathways are important in maintaining lipid balance and cardiovascular health. Apo-CI is related to other apolipoproteins within these pathways such as apo E and apo A-I. Its function in modulating lipoprotein receptor interactions aligns with the broader mechanisms of these pathways in lipid and cholesterol regulation.

Elevated levels of Apo-CI are associated with hyperlipidemia and cardiovascular disease. These conditions may result from its inhibitory effects on lipase activity leading to increased triglyceride levels. Apo-CI's interaction with other apolipoproteins such as apo B and apo E is linked to its role in the pathogenesis of these disorders. Understanding Apo-CI's involvement in these diseases can guide therapeutic strategies aimed at regulating its expression and function.
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Product protocols

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Target data

Inhibitor of lipoprotein binding to the low density lipoprotein (LDL) receptor, LDL receptor-related protein, and very low density lipoprotein (VLDL) receptor. Associates with high density lipoproteins (HDL) and the triacylglycerol-rich lipoproteins in the plasma and makes up about 10% of the protein of the VLDL and 2% of that of HDL. Appears to interfere directly with fatty acid uptake and is also the major plasma inhibitor of cholesteryl ester transfer protein (CETP). Binds free fatty acids and reduces their intracellular esterification. Modulates the interaction of APOE with beta-migrating VLDL and inhibits binding of beta-VLDL to the LDL receptor-related protein.
See full target information APOC1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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