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AB108808

Human Apolipoprotein CI ELISA Kit (Apo C1)

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(5 Publications)

Human Apolipoprotein CI ELISA Kit (Apo C1) is a Sandwich (quantitative) ELISA for the measurement of Human Apolipoprotein CI (Apo C1) in Human in Biofluids samples.

View Alternative Names

Apolipoprotein C-I, Apo-CI, ApoC-I, Apolipoprotein C1, APOC1

1 Images
Sandwich ELISA - Human Apolipoprotein CI ELISA Kit (Apo C1) (AB108808)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CI ELISA Kit (Apo C1) (AB108808)

Representative Standard Curve using ab108808

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 36 ng/mL

Range

0.063 - 4 µg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Apolipoprotein CI (APOCI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein CI concentrations in plasma and serum samples.

An Apolipoprotein CI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CI captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108808-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108808-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB108808-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108808-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Apolipoprotein CI Microplate (12 x 8 well strips)", "number":"AB108808-CMP04", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108808-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Apolipoprotein CI Antibody", "number":"AB108808-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108808-CMP09", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108808-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Apolipoprotein CI Standard", "number":"AB108808-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein CI (Apo-CI) plays a mechanical role in lipid metabolism where it acts as an inhibitor of lipoprotein lipase and hepatic lipase hindering lipolytic activity. It is also known as 'apo 250' and 'apo atv20' with a molecular mass of approximately 6.6 kDa. Apo-CI is primarily expressed in the liver and secreted into the plasma where it associates with high-density lipoproteins (HDL) and very low-density lipoproteins (VLDL).
Biological function summary

Apo-CI influences plasma triglyceride levels and cholesterol homeostasis. It plays a role in lipid transport and metabolism by modulating the binding of other apolipoproteins such as apo A-IV and interfering with the binding of lipoproteins to their receptors. Apo-CI is part of a complex of apolipoproteins that regulate lipid metabolism working in tandem to balance lipid and cholesterol levels in the body.

Pathways

Apo-CI is actively involved in the lipoprotein metabolism pathway and cholesterol efflux pathway. These pathways are important in maintaining lipid balance and cardiovascular health. Apo-CI is related to other apolipoproteins within these pathways such as apo E and apo A-I. Its function in modulating lipoprotein receptor interactions aligns with the broader mechanisms of these pathways in lipid and cholesterol regulation.

Elevated levels of Apo-CI are associated with hyperlipidemia and cardiovascular disease. These conditions may result from its inhibitory effects on lipase activity leading to increased triglyceride levels. Apo-CI's interaction with other apolipoproteins such as apo B and apo E is linked to its role in the pathogenesis of these disorders. Understanding Apo-CI's involvement in these diseases can guide therapeutic strategies aimed at regulating its expression and function.
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Product protocols

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Target data

Inhibitor of lipoprotein binding to the low density lipoprotein (LDL) receptor, LDL receptor-related protein, and very low density lipoprotein (VLDL) receptor. Associates with high density lipoproteins (HDL) and the triacylglycerol-rich lipoproteins in the plasma and makes up about 10% of the protein of the VLDL and 2% of that of HDL. Appears to interfere directly with fatty acid uptake and is also the major plasma inhibitor of cholesteryl ester transfer protein (CETP). Binds free fatty acids and reduces their intracellular esterification. Modulates the interaction of APOE with beta-migrating VLDL and inhibits binding of beta-VLDL to the LDL receptor-related protein.
See full target information APOC1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 14:1181435 PubMed37305534

2023

APOC1 exacerbates renal fibrosis through the activation of the NF-κB signaling pathway in IgAN.

Applications

Unspecified application

Species

Unspecified reactive species

Kuipeng Yu,Lin Ding,Xin An,Yanjiang Yang,Xiaoning Zhang,Luyao Li,Chunjie Wang,Fang Bai,Xiangdong Yang

Frontiers in endocrinology 14:1102634 PubMed36891052

2023

APOC1 as a novel diagnostic biomarker for DN based on machine learning algorithms and experiment.

Applications

Unspecified application

Species

Unspecified reactive species

Kuipeng Yu,Shan Li,Chunjie Wang,Yimeng Zhang,Luyao Li,Xin Fan,Lin Fang,Haiyun Li,Huimin Yang,Jintang Sun,Xiangdong Yang

Journal of lipid research 63:100144 PubMed34710432

2021

Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Oleg Kovrov,Fredrik Landfors,Valeria Saar-Kovrov,Ulf Näslund,Gunilla Olivecrona

Biomarkers in medicine 14:839-854 PubMed32856461

2020

iTRAQ-based comparative proteomics analysis reveals specific urinary biomarkers for various kidney diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Jin,Jianguang Gong,Li Zhao,Yiwen Li,Yunguang Wang,Qiang He

Proteomics. Clinical applications 12:e1700164 PubMed29754444

2018

Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Yang,Yanni Chen,Xiaofan Xiong,Xiaobo Zhou,Lin Han,Lei Ni,Wenjing Wang,Xiaofei Wang,Lingyu Zhao,Dongdong Shao,Chen Huang
View all publications
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