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AB309397

Human Apolipoprotein CII/ApoC-II ELISA Kit

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Human Apolipoprotein CII/ApoC-II ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Apolipoprotein CII/ApoC-II in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

APC2, APOC2, Apolipoprotein C-II, Apo-CII, ApoC-II, Apolipoprotein C2

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Sandwich ELISA - Human Apolipoprotein CII/ApoC-II ELISA Kit (AB309397)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CII/ApoC-II ELISA Kit (AB309397)

Example of standard curve using ab309397. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.5 ng/mL

Range

1.5 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Apolipoprotein CII/ApoC-II ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Apolipoprotein CII/ApoC-II in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Apolipoprotein CII/ApoC-II coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "82 - 97 %", "average": "= 88.5" }, { "sample": "Serum", "range": "101 - 121 %", "average": "= 107.4" }, { "sample": "Cell culture media", "range": "80 - 97 %", "average": "= 88.41" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309397-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309397-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309397-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309397-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ApoC2 Antibody-coated ELISA Plate", "number":"AB309397-CMP09", "productcode":"" }, { "size":"2 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309397-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ApoC2 Protein Standard", "number":"AB309397-CMP11", "productcode":"" }, { "size":"1 x 200 µL", "name":"1200X HRP-Streptavidin", "number":"AB309397-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309397-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ApoC2 Detection Antibody", "number":"AB309397-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein CII also known as ApoC-II is an essential player in lipid metabolism. It is a 79 amino acid protein with an approximate mass of 8.9 kDa. ApoC-II is primarily expressed in the liver and secreted into the bloodstream where it associates with very-low-density lipoproteins (VLDLs) and chylomicrons. This protein serves an important mechanical role as an activator of lipoprotein lipase (LPL) an enzyme that hydrolyzes triglycerides into free fatty acids facilitating their uptake by tissues for energy production or storage.
Biological function summary

ApoC-II plays a critical role in the metabolism of plasma triglycerides. As an important component of the lipoprotein complex it is necessary for effective lipid transport and triglyceride regulation. By activating lipoprotein lipase ApoC-II ensures efficient breakdown of triglycerides in the bloodstream aiding in maintaining lipid homeostasis. Without ApoC-II LPL lacks full activity leading to impaired triglyceride clearance and dyslipidemia.

Pathways

ApoC-II is integral to lipid metabolic pathways particularly in the catabolism of triglyceride-rich lipoproteins. It participates significantly in the lipid digestion pathway where it functions in concert with proteins like ApoE and ApoB which further assist in lipid binding and transport. ApoC-II's regulatory actions within these pathways are important for preventing the build-up of triglycerides in circulation contributing to a balance in energy storage and metabolism.

Mutations or deficiencies in ApoC-II function can lead to hyperlipoproteinemia type Ib a condition characterized by elevated plasma triglycerides and risk of pancreatitis. This disorder manifests due to defective activation of lipoprotein lipase highlighting the target's role alongside proteins such as LPL in lipid clearance. Additionally ApoC-II interactions with cholesterol transport proteins may influence atherosclerosis a condition where plaque builds up in arteries posing further cardiovascular risks.
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Product protocols

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Target data

Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
See full target information APOC2
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