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AB168549

Human Apolipoprotein CII ELISA Kit

5

(1 Review)

|

(3 Publications)

Human Apolipoprotein CII ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Apolipoprotein CII in Human in Biofluids samples.

View Alternative Names

APC2, APOC2, Apolipoprotein C-II, Apo-CII, ApoC-II, Apolipoprotein C2

1 Images
Sandwich ELISA - Human Apolipoprotein CII ELISA Kit (AB168549)
  • sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CII ELISA Kit (AB168549)

Representative Standard Curve using ab168549

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7 ng/mL

Range

0.016 - 4 µg/mL

Assay time

4h

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Apolipoprotein CII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein CII concentrations in plasma and serum.

An Apolipoprotein CII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CII captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein CII also known as ApoC-II is an essential player in lipid metabolism. It is a 79 amino acid protein with an approximate mass of 8.9 kDa. ApoC-II is primarily expressed in the liver and secreted into the bloodstream where it associates with very-low-density lipoproteins (VLDLs) and chylomicrons. This protein serves an important mechanical role as an activator of lipoprotein lipase (LPL) an enzyme that hydrolyzes triglycerides into free fatty acids facilitating their uptake by tissues for energy production or storage.
Biological function summary

ApoC-II plays a critical role in the metabolism of plasma triglycerides. As an important component of the lipoprotein complex it is necessary for effective lipid transport and triglyceride regulation. By activating lipoprotein lipase ApoC-II ensures efficient breakdown of triglycerides in the bloodstream aiding in maintaining lipid homeostasis. Without ApoC-II LPL lacks full activity leading to impaired triglyceride clearance and dyslipidemia.

Pathways

ApoC-II is integral to lipid metabolic pathways particularly in the catabolism of triglyceride-rich lipoproteins. It participates significantly in the lipid digestion pathway where it functions in concert with proteins like ApoE and ApoB which further assist in lipid binding and transport. ApoC-II's regulatory actions within these pathways are important for preventing the build-up of triglycerides in circulation contributing to a balance in energy storage and metabolism.

Mutations or deficiencies in ApoC-II function can lead to hyperlipoproteinemia type Ib a condition characterized by elevated plasma triglycerides and risk of pancreatitis. This disorder manifests due to defective activation of lipoprotein lipase highlighting the target's role alongside proteins such as LPL in lipid clearance. Additionally ApoC-II interactions with cholesterol transport proteins may influence atherosclerosis a condition where plaque builds up in arteries posing further cardiovascular risks.
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Product protocols

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Target data

Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
See full target information APOC2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 63:100144 PubMed34710432

2021

Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Oleg Kovrov,Fredrik Landfors,Valeria Saar-Kovrov,Ulf Näslund,Gunilla Olivecrona

Arteriosclerosis, thrombosis, and vascular biology 39:63-72 PubMed30580564

2018

Effects of APOC3 Heterozygous Deficiency on Plasma Lipid and Lipoprotein Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Gissette Reyes-Soffer,Carol Sztalryd,Richard B Horenstein,Stephen Holleran,Anastasiya Matveyenko,Tiffany Thomas,Renu Nandakumar,Colleen Ngai,Wahida Karmally,Henry N Ginsberg,Rajasekhar Ramakrishnan,Toni I Pollin

Atherosclerosis 253:171-177 PubMed27451002

2016

Short-term isocaloric fructose restriction lowers apoC-III levels and yields less atherogenic lipoprotein profiles in children with obesity and metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Gugliucci,Robert H Lustig,Russell Caccavello,Ayca Erkin-Cakmak,Susan M Noworolski,Viva W Tai,Michael J Wen,Kathleen Mulligan,Jean-Marc Schwarz
View all publications
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