JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154131

Human Apolipoprotein CIII ELISA Kit (APOC3)

5

(3 Reviews)

|

(16 Publications)

Human Apolipoprotein CIII ELISA Kit (APOC3) is a sandwich ELISA used to quantify Human APO C 3 with a sensitivity of 1.1 ng/mL.

- Colorimetric sandwich ELISA - 450nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide measuring range: 1.953 - 500 ng/mL
- Cited in over 10 publications

View Alternative Names

Apolipoprotein C-III, Apo-CIII, ApoC-III, Apolipoprotein C3, APOC3

3 Images
Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (AB154131)
  • sELISA

Unknown

Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (AB154131)

Standard curve of Hu APOCIII, with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (AB154131)
  • sELISA

Unknown

Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (AB154131)

APOCIII measured in biological fluids, background signal subtracted (duplicates +/- SD).

Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (AB154131)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (AB154131)

Representative Standard Curve using ab154131.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Urine, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 ng/mL

Range

1.953 - 500 ng/mL

Assay time

4h

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB233623

Human Apo E ELISA Kit

Recombinant|SimpleStep

ELISA - Human Apo E ELISA Kit (AB233623)

  • 0
  • 0
Assay Kits

AB190806

Human Apolipoprotein B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Apolipoprotein B ELISA Kit (AB190806)

  • 4
  • 1
Assay Kits

AB212165

Human Lipoprotein A ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)

  • 5
  • 1
Assay Kits

AB184859

Human Apolipoprotein AII ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Apolipoprotein AII ELISA Kit (AB184859)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Apolipoprotein CIII ELISA Kit (APOC3) ab154131 is a sandwich ELISA to measure Human Apolipoprotein CIII (APOC3) in cerebral spinal fluid, Urine, plasma, milk, serum with a sensitivity of 1.1 ng/mL

How the assay works:

An Apolipoprotein CIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CIII captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Apolipoprotein CIII ELISA Kit (APOC3) ab154131 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature.
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature.
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab154131-1x96Tests", "assetComponentDetails": [ { "size":"1 x 150 µL", "name":"40X Biotinylated Human Apolipoprotein CIII Antibody", "number":"AB154131-CMP01", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB154131-CMP06", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB154131-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Apolipoprotein CIII Standard", "number":"AB154131-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB154131-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB154131-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Apolipoprotein CIII Microplate (12 x 8 well strips)", "number":"AB154131-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB154131-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB154131-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein CIII also known as Apo C-III or ApoC3 is an apolipoprotein with a molecular mass of approximately 8.8 kDa. It is synthesized in the liver and intestine and circulates in the bloodstream primarily as part of very low-density lipoproteins (VLDL) and high-density lipoproteins (HDL). Its main role involves the regulation of lipid metabolism particularly in relation to triglyceride-rich particles. Apo C-III serves as an inhibitor of lipoprotein lipase an enzyme essential for the hydrolysis of triglycerides into free fatty acids.
Biological function summary

Apolipoprotein CIII plays a pivotal role in lipid and lipoprotein metabolism. It is not part of a large protein complex but interacts with lipoproteins as a modulatory protein. Apo C-III affects triglyceride metabolism by delaying the clearance of VLDL and enhancing hepatic triglyceride secretion. Additionally it modulates the uptake of lipoprotein remnants by liver receptors thereby influencing the plasma levels of triglycerides and cholesterol.

Pathways

Apolipoprotein CIII is involved in the regulation of lipid metabolic pathways. It plays a role in the lipoprotein assembly pathway and the triglyceride biosynthetic process. This protein impacts the interaction between lipoprotein lipase and its substrates hindering enzyme activity which leads to elevated plasma triglyceride levels. It relates closely to other apolipoproteins such as apolipoprotein E which also participates in lipid transport and metabolism although with distinct functions and regulatory mechanisms.

Apolipoprotein CIII is implicated in conditions like hypertriglyceridemia and cardiovascular disease. Elevated levels of Apo C-III contribute to high triglyceride levels promoting atherosclerosis development due to the retention of triglyceride-rich lipoproteins in the bloodstream. This association links it to cardiovascular disease risk where its role contrasts with proteins like apolipoprotein AI which is mainly involved in protective effects via HDL metabolism. Understanding the modulation of Apo C-III levels offers potential therapeutic avenues for managing these lipid-related disorders.
style
left-column

Product protocols

style
left-column

Target data

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma (PubMed : 18201179, PubMed : 22510806). Plays a multifaceted role in triglyceride homeostasis (PubMed : 18201179, PubMed : 22510806). Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs) (PubMed : 18201179, PubMed : 22510806). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors (PubMed : 18201179, PubMed : 22510806). Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners (PubMed : 18408013).
See full target information Apolipoprotein C-III
style
left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Lipids in health and disease 23:59 PubMed38414008

2024

Pre-operative levels of angiopoietin protein-like 3 (ANGPTL3) in women diagnosed with high-grade serous carcinoma of the ovary.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Wong Chong,France-Hélène Joncas,Pierre Douville,Dimcho Bachvarov,Caroline Diorio,Frédéric Calon,Ann-Charlotte Bergeron,Jonatan Blais,Shuk On Annie Leung,Nabil Georges Seidah,Anne Gangloff

Nature immunology 24:408-411 PubMed36781985

2023

Apolipoprotein C3 induces inflammasome activation only in its delipidated form.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Chieh Hsu,Baohai Shao,Jenny E Kanter,Yi He,Tomas Vaisar,Joseph L Witztum,Janet Snell-Bergeon,Gregory McInnes,Shannon Bruse,Omri Gottesman,Adam E Mullick,Karin E Bornfeldt

Frontiers in immunology 13:855513 PubMed35677050

2022

Proteomic Profiling of Cryoglobulinemia.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Liu,Jianqiang Wu,Dandan Sun,Haolong Li,Zhihong Qi,Xiaoyue Tang,Wei Su,Yongzhe Li,Xuzhen Qin

Journal of lipid research 63:100144 PubMed34710432

2021

Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Oleg Kovrov,Fredrik Landfors,Valeria Saar-Kovrov,Ulf Näslund,Gunilla Olivecrona

Biochimica et biophysica acta. Molecular and cell biology of lipids 1867:159064 PubMed34610468

2021

Heparin binding triggers human VLDL remodeling by circulating lipoprotein lipase: Relevance to VLDL functionality in health and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shobini Jayaraman,Antonio Pérez,Inka Miñambres,Jose Luis Sánchez-Quesada,Olga Gursky

Journal of internal medicine 290:1249-1263 PubMed34337800

2021

Lipid profiles of patients with manifest coronary versus peripheral atherosclerosis - Is there a difference?

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Leiherer,Axel Mündlein,Eva Maria Brandtner,Christoph H Säly,Hana Ramadani,Alexander Vonbank,Arthur Mader,Jörn F Dopheide,Antti Jylhä,Mitja Lääperi,Reijo Laaksonen,Winfried März,Peter Fraunberger,Marcus Kleber,Heinz Drexel

Clinical biochemistry 79:14-22 PubMed32045574

2020

HDL-associated apoCIII plays an independent role in predicting postprandial hypertriglyceridemia.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhua Zhang,Xiaoyu Tang,Ling Mao,Jin Chen,Jie Kuang,Xin Guo,Danyan Xu,Daoquan Peng,Bilian Yu

Arteriosclerosis, thrombosis, and vascular biology 39:63-72 PubMed30580564

2018

Effects of APOC3 Heterozygous Deficiency on Plasma Lipid and Lipoprotein Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Gissette Reyes-Soffer,Carol Sztalryd,Richard B Horenstein,Stephen Holleran,Anastasiya Matveyenko,Tiffany Thomas,Renu Nandakumar,Colleen Ngai,Wahida Karmally,Henry N Ginsberg,Rajasekhar Ramakrishnan,Toni I Pollin

Lipids in health and disease 17:160 PubMed30021607

2018

Apolipoprotein CIII may mediate the impacts of angiopoietin-like protein 8 on triglyceride metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdie Luo,Xin Su,Yuhong Yi,Yang Yang,Daoquan Peng

Scientific reports 7:2312 PubMed28539597

2017

ApoCIII enrichment in HDL impairs HDL-mediated cholesterol efflux capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdie Luo,Aiying Liu,Shuai Wang,Tianle Wang,Die Hu,Sha Wu,Daoquan Peng
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com