Human Apolipoprotein CIII ELISA Kit (APOC3) ab154131 is used to quantify Human Apolipoprotein CIII with a sensitivity of 8.68 pg/ml.
Sandwich ELISA Kit
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- Nature immunology 24:408-4112023Apolipoprotein C3 induces inflammasome activation only in its delipidated form.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng-Chieh Hsu,Baohai Shao,Jenny E Kanter,Yi He,Tomas Vaisar,Joseph L Witztum,Janet Snell-Bergeon,Gregory McInnes,Shannon Bruse,Omri Gottesman,Adam E Mullick,Karin E BornfeldtPubMed 36781985
- Frontiers in immunology 13:8555132022Proteomic Profiling of Cryoglobulinemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Liu,Jianqiang Wu,Dandan Sun,Haolong Li,Zhihong Qi,Xiaoyue Tang,Wei Su,Yongzhe Li,Xuzhen QinPubMed 35677050
- Journal of lipid research 63:1001442021Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOleg Kovrov,Fredrik Landfors,Valeria Saar-Kovrov,Ulf Näslund,Gunilla OlivecronaPubMed 34710432
- Journal of internal medicine 290:1249-12632021Lipid profiles of patients with manifest coronary versus peripheral atherosclerosis - Is there a difference?Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndreas Leiherer,Axel Mündlein,Eva Maria Brandtner,Christoph H Säly,Hana Ramadani,Alexander Vonbank,Arthur Mader,Jörn F Dopheide,Antti Jylhä,Mitja Lääperi,Reijo Laaksonen,Winfried März,Peter Fraunberger,Marcus Kleber,Heinz DrexelPubMed 34337800
- Clinical biochemistry 79:14-222020HDL-associated apoCIII plays an independent role in predicting postprandial hypertriglyceridemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianhua Zhang et. al.PubMed 32045574
- Arteriosclerosis, thrombosis, and vascular biology 39:63-722018Effects of APOC3 Heterozygous Deficiency on Plasma Lipid and Lipoprotein Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGissette Reyes-Soffer,Carol Sztalryd,Richard B Horenstein,Stephen Holleran,Anastasiya Matveyenko,Tiffany Thomas,Renu Nandakumar,Colleen Ngai,Wahida Karmally,Henry N Ginsberg,Rajasekhar Ramakrishnan,Toni I PollinPubMed 30580564
- Lipids in health and disease 17:1602018Apolipoprotein CIII may mediate the impacts of angiopoietin-like protein 8 on triglyceride metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengdie Luo et. al.PubMed 30021607
- Scientific reports 7:23122017ApoCIII enrichment in HDL impairs HDL-mediated cholesterol efflux capacity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengdie Luo et. al.PubMed 28539597
- Journal of lipid research 58:1214-12202017Effects of CETP inhibition with anacetrapib on metabolism of VLDL-TG and plasma apolipoproteins C-II, C-III, and E.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn S Millar et. al.PubMed 28314859
- The Journal of biological chemistry 292:3692-37052017APOC3 Protein Is Not a Predisposing Factor for Fat-induced Nonalcoholic Fatty Liver Disease in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyun Cheng et. al.PubMed 28115523
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Urine, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.953 - 500 ng/mL
- Assay time
- 4h
- Sensitivity
- = 1.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma (PubMed:18201179, PubMed:22510806). Plays a multifaceted role in triglyceride homeostasis (PubMed:18201179, PubMed:22510806). Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs) (PubMed:18201179, PubMed:22510806). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors (PubMed:18201179, PubMed:22510806). Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners (PubMed:18408013).
Alternative names
Apolipoprotein C-III, Apo-CIII, ApoC-III, Apolipoprotein C3, APOC3
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Human Apolipoprotein CIII ELISA Kit (APOC3) ab154131 is used to quantify Human Apolipoprotein CIII with a sensitivity of 8.68 pg/ml.
Sandwich ELISA Kit
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Urine, Plasma, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.953 - 500 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 1.1 ng/mL
- General recovery
- 99%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Human Apolipoprotein CIII (APOC3) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab154131) is designed for the quantitative measurement of APOC3 in plasma, serum, urine, milk, CSF, and cell culture samples.
An Apolipoprotein CIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CIII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein CIII also known as Apo C-III or ApoC3 is an apolipoprotein with a molecular mass of approximately 8.8 kDa. It is synthesized in the liver and intestine and circulates in the bloodstream primarily as part of very low-density lipoproteins (VLDL) and high-density lipoproteins (HDL). Its main role involves the regulation of lipid metabolism particularly in relation to triglyceride-rich particles. Apo C-III serves as an inhibitor of lipoprotein lipase an enzyme essential for the hydrolysis of triglycerides into free fatty acids.
- Biological function summary
Apolipoprotein CIII plays a pivotal role in lipid and lipoprotein metabolism. It is not part of a large protein complex but interacts with lipoproteins as a modulatory protein. Apo C-III affects triglyceride metabolism by delaying the clearance of VLDL and enhancing hepatic triglyceride secretion. Additionally it modulates the uptake of lipoprotein remnants by liver receptors thereby influencing the plasma levels of triglycerides and cholesterol.
- Pathways
Apolipoprotein CIII is involved in the regulation of lipid metabolic pathways. It plays a role in the lipoprotein assembly pathway and the triglyceride biosynthetic process. This protein impacts the interaction between lipoprotein lipase and its substrates hindering enzyme activity which leads to elevated plasma triglyceride levels. It relates closely to other apolipoproteins such as apolipoprotein E which also participates in lipid transport and metabolism although with distinct functions and regulatory mechanisms.
- Associated diseases and disorders
Apolipoprotein CIII is implicated in conditions like hypertriglyceridemia and cardiovascular disease. Elevated levels of Apo C-III contribute to high triglyceride levels promoting atherosclerosis development due to the retention of triglyceride-rich lipoproteins in the bloodstream. This association links it to cardiovascular disease risk where its role contrasts with proteins like apolipoprotein AI which is mainly involved in protective effects via HDL metabolism. Understanding the modulation of Apo C-III levels offers potential therapeutic avenues for managing these lipid-related disorders.
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3 product images
Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (ab154131)
Representative Standard Curve using ab154131.
Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (ab154131)
APOCIII measured in biological fluids, background signal subtracted (duplicates +/- SD).
Sandwich ELISA - Human Apolipoprotein CIII ELISA Kit (APOC3) (ab154131)
Standard curve of Hu APOCIII, with background signal subtracted (duplicates; +/- SD).
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