AB325560

Human Apolipoprotein CIII High Sensitivity ELISA Kit

SimpleStep
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Human Apolipoprotein CIII High Sensitivity ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein CIII High Sensitivity with a sensitivity of 141.2pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Validated on a number of sample types including cerebrospinal fluid (CSF).

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

View Alternative Names

Apolipoprotein C-III, Apo-CIII, ApoC-III, Apolipoprotein C3, APOC3

7 Images

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

Example of human Apolipoprotein C-III standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

Interpolated concentration of native Apolipoprotein C-III was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 10%, milk 2%, cerebrospinal fluid 2.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

Interpolated concentration of native Apolipoprotein C-III was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 50,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

Example of human Apolipoprotein C-III standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

Serum of four individual Alzheimer’s Disease human donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Apolipoprotein C-III concentration was determined to be 180.7 µg/mL with a range of 164.6 – 188.4 µg/mL.

sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Apolipoprotein C-III concentration was determined to be 214.1 µg/mL with a range of 123.5 – 310.7 µg/mL.

sELISA

Lab

Sandwich ELISA - Human Apolipoprotein CIII High Sensitivity ELISA Kit (AB325560)

Interpolated concentration of native Apolipoprotein C-III was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 50,000, plasma (citrate) 1 : 33,333, plasma (EDTA) 1 : 66,667, and plasma (heparin) 1 : 33,333. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Cerebral Spinal Fluid, Milk, Urine, Heparin Plasma, EDTA Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 141.2 pg/mL

Range

218.75 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Apolipoprotein CIII High Sensitivity ELISA Kit (ab325560) is a rapid single-wash sandwich 90-min ELISA to measure Human Apolipoprotein CIII High Sensitivity in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Urine, Milk, Cerebrospinal Fluid. This SimpleStep sensitivity is 141.2 pg/ml.

How the assay works

Human Apolipoprotein CIII High Sensitivity ELISA Kit (ab325560) SimpleStep ELISA^® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Apolipoprotein CIII High Sensitivity (ab325560) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samplesOur Human Apolipoprotein CIII High Sensitivity ELISA Kit only requires 12.5µL sample.

Design your own immunoassay

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Precision

[ { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.1" }, { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 13.1" } ]

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Recovery

[ { "sample": "Serum", "range": "112 - 119 %", "average": "= 117" }, { "sample": "Citrate plasma", "range": "96 - 111 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "113 - 116 %", "average": "= 115" }, { "sample": "Heparin Plasma", "range": "88 - 109 %", "average": "= 102" }, { "sample": "Urine", "range": "107 - 114 %", "average": "= 111" }, { "sample": "Milk", "range": "110 - 115 %", "average": "= 112" }, { "sample": "Cerebral Spinal Fluid", "range": "106 - 110 %", "average": "= 108" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma (PubMed : 18201179, PubMed : 22510806). Plays a multifaceted role in triglyceride homeostasis (PubMed : 18201179, PubMed : 22510806). Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs) (PubMed : 18201179, PubMed : 22510806). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors (PubMed : 18201179, PubMed : 22510806). Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners (PubMed : 18408013).

See full target information Apolipoprotein C-III

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websiteProtocolBooklet

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Product promise

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