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Human Apolipoprotein E ELISA Kit (APOE) ab108813 is used to quantify Human Apolipoprotein E with a sensitivity of 0.28 ng/ml.Sandwich ELISA Kit


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Sandwich ELISA - Human Apolipoprotein E ELISA Kit (APOE) (AB108813), expandable thumbnail
  • Sandwich ELISA - Human Apolipoprotein E ELISA Kit (APOE) (AB108813), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.008 - 0.5 µg/mL

Assay time

4h

Sensitivity

= 4 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids (PubMed:14754908, PubMed:1911868, PubMed:6860692). APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance (PubMed:14754908, PubMed:1911868, PubMed:1917954, PubMed:23620513, PubMed:2762297, PubMed:6860692, PubMed:9395455). Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma (PubMed:2762297, PubMed:6860692, PubMed:9395455). As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) (PubMed:1911868, PubMed:6860692). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (PubMed:12950167, PubMed:1530612, PubMed:1917954, PubMed:20030366, PubMed:20303980, PubMed:2063194, PubMed:2762297, PubMed:7635945, PubMed:7768901, PubMed:8756331, PubMed:8939961). Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed:23676495, PubMed:7635945, PubMed:9395455, PubMed:9488694). A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes (PubMed:1911868, PubMed:1917954, PubMed:23676495, PubMed:29516132, PubMed:9395455). APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues (PubMed:2762297, PubMed:29516132). By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis (PubMed:1917954, PubMed:2762297, PubMed:29516132). APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis (PubMed:14754908, PubMed:23620513, PubMed:9395455). First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues (PubMed:14754908, PubMed:23620513). Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes (PubMed:9395455). APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting (PubMed:25173806, PubMed:8939961). APOE is also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells (By similarity). Binds to the immune cell receptor LILRB4 (PubMed:30333625). APOE may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP (PubMed:28111074).(Microbial infection) Through its interaction with HCV envelope glycoprotein E2, participates in the attachment of HCV to HSPGs and other receptors (LDLr, VLDLr, and SR-B1) on the cell surface and to the assembly, maturation and infectivity of HCV viral particles (PubMed:25122793, PubMed:29695434). This interaction is probably promoted via the up-regulation of cellular autophagy by the virus (PubMed:29695434).

Alternative names

What's included?

1 x 96 Tests
Components
100X Streptavidin-Peroxidase Conjugate
1 x 80 µL
10X Diluent M Concentrate
1 x 30 mL
1X Standard Diluent
1 x 2 mL
20X Wash Buffer Concentrate
2 x 30 mL
70X Biotinylated Human Apolipoprotein E Antibody
1 x 90 µL
Apolipoprotein E Microplate (12 x 8 well strips)
1 x 1 Unit
Apolipoprotein E Standard
2 x 1 Vial
Chromogen Substrate
1 x 7 mL
Sealing Tapes
3 x 1 Unit
Stop Solution
1 x 11 mL

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Human Apolipoprotein E ELISA Kit (APOE) ab108813 is used to quantify Human Apolipoprotein E with a sensitivity of 0.28 ng/ml.Sandwich ELISA Kit

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.008 - 0.5 µg/mL

Assay time

4h

Assay Platform

Microplate

Sensitivity

= 4 ng/mL

General recovery

97%

Precision

Intra assay

Sample

Overall

n
0
mean
-
SD
-
C.V.

6.3

Inter assay

Sample

Overall

n
0
mean
-
SD
-
C.V.

10.6

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Human Apolipoprotein E ELISA kit (ApoE) is designed for the quantitative measurement of Apolipoprotein E concentrations in human plasma, serum, cerebrospinal fluid and cell culture supernatant samples.

An Apolipoprotein E specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein E specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein E captured in plate. The kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.

Biological function summary

Four words that are not the target name include ApoE's role as a constituent of chylomicrons VLDL and HDL particles. ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.

Pathways

Four words that are not the target name include the involvement of ApoE in lipid metabolism and cardiovascular disease pathways. In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.

Associated diseases and disorders

Four words that are not the target name include ApoE's association with Alzheimer’s disease and cardiovascular diseases. In Alzheimer’s disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.

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2 product images

  • Sandwich ELISA - Human Apolipoprotein E ELISA Kit (APOE) (ab108813), expandable thumbnail

    Sandwich ELISA - Human Apolipoprotein E ELISA Kit (APOE) (ab108813)

    Apolipoprotein E measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).

  • Sandwich ELISA - Human Apolipoprotein E ELISA Kit (APOE) (ab108813), expandable thumbnail

    Sandwich ELISA - Human Apolipoprotein E ELISA Kit (APOE) (ab108813)

    Representative Standard Curve using ab108813.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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