AB283991

Human APRT ELISA Kit

Name: Human APRT ELISA Kit (ab283991) | Abcam
Brand: Abcam Limited
SKU: AB283991

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Human APRT ELISA Kit is a Sandwich (qualitative) ELISA for the measurement of Human APRT in Human in Cell/Tissue Extracts, Biofluids, Cell Culture Media samples.

View Alternative Names

Adenine phosphoribosyltransferase, APRT

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Sandwich ELISA - Human APRT ELISA Kit (AB283991)

sELISA

Supplier Data

Sandwich ELISA - Human APRT ELISA Kit (AB283991)

Example of Human APRT standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich (qualitative)

Sensitivity

= 25 pg/mL

Range

0.075 - 4.8 ng/mL

Assay time

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The Human APRT ELISA Kit (ab283991) is designed for detection of APRT in human plasma, serum, cell culture, and cell lysate samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human APRT in approximately 4 hours. A polyclonal antibody specific for human APRT has been pre-coated onto a 96-well microplate with removable strips. APRT in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human APRT, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenine phosphoribosyltransferase (APRT) also known as APTR is an enzyme involved in the purine salvage pathway. It has a molecular mass of approximately 23 kDa and gets expressed in numerous tissues including the liver kidney and brain. This protein catalyzes the conversion of adenine and phosphoribosyl pyrophosphate (PRPP) to adenosine monophosphate (AMP) facilitating the reutilization of adenine into nucleotides which is necessary for DNA and RNA synthesis.

Biological function summary

Adenine phosphoribosyltransferase plays a role in cellular nucleotide pool maintenance by rescuing adenine from degradation and incorporating it back into cellular metabolism. This enzyme does not form part of a larger enzyme complex but acts independently to perform its catalytic function. Properly maintaining nucleotide balance is fundamental for cellular activities and imbalances can lead to cellular stress or DNA damage responses.

Pathways

The purine salvage pathway is where adenine phosphoribosyltransferase functions critically. This pathway operates alongside the de novo synthesis of purine nucleotides working to conserve energy and resources during nucleotide synthesis. APRT interacts closely with hypoxanthine-guanine phosphoribosyltransferase (HGPRT) within this salvage process to recycle purines effectively reducing the need for energy-expensive de novo synthesis.

Defects in the adenine phosphoribosyltransferase gene can result in the condition known as APRT deficiency. This genetic disorder leads to the buildup of 28-dihydroxyadenine causing nephrolithiasis and potential kidney damage. APRT's function connects with xanthine oxidase activity influencing uric acid metabolism. The accumulation of poorly soluble adenine derivatives can also cause crystalluria and can influence uric acid levels connecting APRT to the pathophysiology of gout in some cases.

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Product protocols

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Target data

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

See full target information Adenine phosphoribosyltransferase

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human APRT ELISA Kit

Sandwich ELISA - Human APRT ELISA Kit (AB283991)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Cell Lines & Lysates

Cell Lines & Lysates

Primary Antibodies

Primary Antibodies

Product promise