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AB290982

Human Aquaporin 4 ELISA Kit - Extracellular

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Human Aquaporin 4 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human Aquaporin 4 with a sensitivity of 1.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Aquaporin-4, AQP-4, Mercurial-insensitive water channel, WCH4, MIWC, AQP4

2 Images
Sandwich ELISA - Human Aquaporin 4 ELISA Kit - Extracellular (AB290982)
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Supplier Data

Sandwich ELISA - Human Aquaporin 4 ELISA Kit - Extracellular (AB290982)

Example of human Aquaporin 4 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Aquaporin 4 ELISA Kit - Extracellular (AB290982)
  • sELISA

Supplier Data

Sandwich ELISA - Human Aquaporin 4 ELISA Kit - Extracellular (AB290982)

Interpolated concentration of native Aquaporin 4 was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.9 pg/mL

Range

3.906 - 250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Aquaporin 4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Aquaporin 4 protein in human serum and CSF. Quantitate Human Aquaporin 4 with 1.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 98 %", "average": "= 90" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 125 %", "average": "= 114" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 4 also known as AQP4 functions as a water channel protein that facilitates the transport of water across cell membranes. It is composed of approximately 323 amino acids resulting in a molecular mass of around 34-38 kDa. This target expresses most in the central nervous system especially in the astrocytes of the brain and spinal cord. Other expression sites include skeletal muscle kidneys and lungs. AQP4 is often studied using various tools including anti-aquaporin 4 antibodies and specific reagents like AQP4 Ab and anti-AQP4.
Biological function summary

Water balance and neural activity regulation are significantly influenced by aquaporin 4. This protein forms a part of the orthogonal array of particles (OAPs) in the plasma membrane which enhances its water permeability function. Through its concentration at the blood-brain barrier AQP4 contributes to maintaining brain homeostasis by regulating osmotic gradients and extracellular space ion concentrations. Its expression and activity may affect critical processes like neuroexcitation and neuroprotection.

Pathways

Aquaporin 4 actively engages in critical water transport and blood-brain barrier integrity pathways. The passive yet selective water transport facilitated by AQP4 impacts pathways including the transcellular pathway that helps in maintaining the homeostatic and osmotic balance important for brain function. Furthermore AQP4 acts in relation to other proteins such as aquaporin 1 and connexin 43 indicating its role in gap junction communication within the brain.

Aquaporin 4 is intricately linked to conditions like neuromyelitis optica (NMO) and cerebral edema. In NMO autoantibodies such as anti-AQP4 target the protein causing demyelination and inflammation of the optic nerve and spinal cord. In cases of cerebral edema an imbalance in AQP4 expression can disrupt water homeostasis leading to exacerbated swelling. AQP4's interaction with proteins like myelin basic protein in these diseases underlines its significance in neurodegeneration and inflammatory processes.
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Product protocols

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Target data

Forms a water-specific channel (PubMed : 19383790, PubMed : 7559426, PubMed : 8601457). Plays an important role in brain water homeostasis (PubMed : 37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
See full target information AQP4
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websiteProtocolBooklet
en
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Complementary Products

Primary Antibodies

AB259318

Anti-Aquaporin 4 antibody [EPR24281-65]

BOND RX™ Validated|KO Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR24281-65] (AB259318)

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Proteins & Peptides

AB225651

Recombinant Human Aquaporin 4 protein (Tagged)

SDS-PAGE - Recombinant Human Aquaporin 4 protein (Tagged) (AB225651)

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