Human Arginase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Arginase 1 with a sensitivity of 17.77 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Medical oncology (Northwood, London, England) 39:2142022SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Tang,Ling Peng,Huai Liu,Tengfei Xiao,Wangning Gu,Hongmin Yang,Hui Wang,Pan ChenPubMed 36175598
- BMC endocrine disorders 22:622022Elevated serum extracellular vesicle arginase 1 in type 2 diabetes mellitus: a cross-sectional study in middle-aged and elderly population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinwei Li,Wen Zhao,Lu Peng,Yu Li,Shaoping Nie,Huahui Yu,Yanwen Qin,Huina ZhangPubMed 35277141
- Frontiers in medicine 9:7953922022The Expansion of Myeloid-Derived Suppressor Cells Correlates With the Severity of Pneumonia in Kidney Transplant Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Peng,Yulin Luo,Quan Zhuang,Junhui Li,Pengpeng Zhang,Min Yang,Yu Zhang,Gangcheng Kong,Ke Cheng,Yingzi MingPubMed 35242775
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 187.5 - 12000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 17.77 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
Alternative names
Arginase-1, Liver-type arginase, Type I arginase, ARG1
What's included?
Recommended products
Human Arginase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Arginase 1 with a sensitivity of 17.77 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 187.5 - 12000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 17.77 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Tissue Extracts | n 8 | C.V. 2.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Tissue Extracts | n 3 | C.V. 4.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 103 | Range 99 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Arginase 1 ELISA Kit (ab230930) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Arginase 1 protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Arginase 1 with 17.77 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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2 product images
Sandwich ELISA - Human Arginase 1 ELISA Kit (ab230930)
Example of human Arginase 1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Arginase 1 ELISA Kit (ab230930)
Interpolated concentrations of native Arginase 1 in human liver extract based on a 5 µg/mL extract load.
The concentrations of Arginase 1 were measured in duplicate and interpolated from the Arginase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Arginase 1 concentration was determined to be 6383.5 pg/mL in 5 μg /mL liver extract.
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