JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326309

Human Arginase 1 ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Human Arginase 1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Arginase 1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 18.29 - 40000 pg/ml with a sensitivity of 9.74pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

4 Images
Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)
  • sELISA

Lab

Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)
  • sELISA

Lab

Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)

Dynamic range comparison between the Chemiluminescent Human Arginase 1 SimpleStep ELISA, colorimetric Human Arginase 1 SimpleStep ELISA (ab230930), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)
  • sELISA

Lab

Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)

Standard curve comparison between the Chemiluminescent Human Arginase 1 SimpleStep ELISA and colorimetric Human Arginase 1 SimpleStep ELISA (ab230930) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)
  • sELISA

Supplier Data

Sandwich ELISA - Human Arginase 1 ELISA Kit, Chemiluminescent (AB326309)

Interpolated concentrations of native Arginase 1 in human liver extract based on a 5 µg/mL extract load.

The concentrations of Arginase 1 were measured in duplicate and interpolated from the Arginase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Arginase 1 concentration was determined to be 6383.5 pg/mL in 5 µg /mL liver extract.

Key facts

Detection method

Luminescent

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9.74 pg/mL

Range

18.29 - 40000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Arginase 1 ELISA Kit, Chemiluminescent (ab326309) is a rapid single-wash sandwich 55-min ELISA to measure Human Arginase 1 in Tissue Extracts with a sensitivity of 9.74 pg/mL.

How the assay works

Human Arginase 1 ELISA Kit, Chemiluminescent (ab326309) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Arginase 1 (ab326309) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Arginase 1 antibody [EPR20759-256_1] - BSA and Aside free (Capture) ab244703
- Anti-Arginase 1 antibody [EPR20759-289] - BSA and Aside free (Detector) ab244970
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" } ]
style
left-column

Recovery

[ { "sample": "Tissue Extracts", "range": "99 - 107 %", "average": "= 103" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326309-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326309-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326309-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326309-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Arginase 1 Detector Antibody", "number":"AB326309-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326309-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326309-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326309-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326309-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326309-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Arginase 1 Capture Antibody", "number":"AB326309-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Arginase 1 Lyophilized Recombinant Protein", "number":"AB326309-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
See full target information ARG1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com