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AB283981

Human ARL2BP ELISA Kit

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Human ARL2BP ELISA Kit is a Sandwich ELISA for the measurement of Human ARL2BP in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

BART, BART1, ARL2BP, ADP-ribosylation factor-like protein 2-binding protein, ARF-like 2-binding protein, ARL2-binding protein, Binder of ARF2 protein 1

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Sandwich ELISA - Human ARL2BP ELISA Kit (AB283981)
  • sELISA

Supplier Data

Sandwich ELISA - Human ARL2BP ELISA Kit (AB283981)

Example of Human ARL2BP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 80 pg/mL

Range

0.234 - 15 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB188322

Anti-ARL2BP antibody [EPR15265-21]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

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Proteins & Peptides

AB101067

Recombinant Human ARL2BP protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human ARL2BP protein (His tag N-Terminus) (AB101067)

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Cell Lines & Lysates

AB258312

Human ARL2BP knockout HeLa cell lysate

Western blot - Human ARL2BP knockout HeLa cell lysate (AB258312)

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Cell Lines & Lysates

AB265269

Human ARL2BP knockout HeLa cell line

Advanced Validation

Western blot - Human ARL2BP knockout HeLa cell line (AB265269)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human ARL2BP ELISA Kit (ab283981) is designed for detection of ARL2BP in human plasma, serum, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human ARL2BP in approximately 4 hours.

A polyclonal antibody specific for human ARL2BP has been pre-coated onto a 96-well microplate with removable strips. ARL2BP in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human ARL2BP, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283981-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283981-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283981-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Chromogen Substrate", "number":"AB283981-CMP04", "productcode":"" }, { "size":"1 x 180 µL", "name":"30X Biotinylated Human ARL2BP Antibody", "number":"AB283981-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB283981-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283981-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283981-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283981-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ARL2BP Microplate", "number":"AB283981-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ARL2BP Standard", "number":"AB283981-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL2BP also known as ADP-ribosylation factor-like 2 binding protein plays an important role in cellular transport mechanisms. This protein weighs approximately 17 kDa. ARL2BP is mainly expressed in tissues like heart skeletal muscle and pancreas. It interacts with microtubules and is involved in intracellular trafficking and cell movement.
Biological function summary

ARL2BP is involved in processes maintaining mitochondrial function. It forms part of a protein complex that includes ARL2 a small GTPase which regulates the stability and functionality of tubulin cofactor E (TBCE). This interaction is important for proper microtubule dynamics and may influence effective protein folding within mitochondria.

Pathways

ARL2BP has important roles in the microtubule and mitochondrial maintenance pathways. It interacts significantly with tubulin and the microtubule assembly connecting to pathways governing cell structure and movement. Additionally ARL2BP's function ties closely with the maintenance of mitochondrial health impacting related proteins like BBS1 and BBS4 in the context of mitochondrial homoeostasis.

Disruptions in ARL2BP functions relate to neurological conditions like retinitis pigmentosa. Altered ARL2BP interactions affect proteins such as ARL2 and TBCE which contribute to disease pathology. Additionally these alterations may correlate with metabolic syndromes due to mitochondrial dysregulation linking ARL2BP to broader metabolic and neurodegenerative diseases.
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Product protocols

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Target data

Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.
See full target information ARL2BP
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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