Human ARL2BP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ARL2BP in Human in Plasma, Serum, Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.234 - 15 ng/mL
- Assay time
- 4h
- Sensitivity
- = 80 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.
Alternative names
BART, BART1, ARL2BP, ADP-ribosylation factor-like protein 2-binding protein, ARF-like 2-binding protein, ARL2-binding protein, Binder of ARF2 protein 1
What's included?
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Human ARL2BP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human ARL2BP in Human in Plasma, Serum, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.234 - 15 ng/mL
- Assay time
- 4h
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 80 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 60 | mean - | SD - | C.V. 4.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 60 | mean - | SD - | C.V. 9.4 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Human ARL2BP ELISA Kit (ab283981) is designed for detection of ARL2BP in human plasma, serum, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human ARL2BP in approximately 4 hours.
A polyclonal antibody specific for human ARL2BP has been pre-coated onto a 96-well microplate with removable strips. ARL2BP in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human ARL2BP, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARL2BP also known as ADP-ribosylation factor-like 2 binding protein plays an important role in cellular transport mechanisms. This protein weighs approximately 17 kDa. ARL2BP is mainly expressed in tissues like heart skeletal muscle and pancreas. It interacts with microtubules and is involved in intracellular trafficking and cell movement.
- Biological function summary
ARL2BP is involved in processes maintaining mitochondrial function. It forms part of a protein complex that includes ARL2 a small GTPase which regulates the stability and functionality of tubulin cofactor E (TBCE). This interaction is important for proper microtubule dynamics and may influence effective protein folding within mitochondria.
- Pathways
ARL2BP has important roles in the microtubule and mitochondrial maintenance pathways. It interacts significantly with tubulin and the microtubule assembly connecting to pathways governing cell structure and movement. Additionally ARL2BP's function ties closely with the maintenance of mitochondrial health impacting related proteins like BBS1 and BBS4 in the context of mitochondrial homoeostasis.
- Associated diseases and disorders
Disruptions in ARL2BP functions relate to neurological conditions like retinitis pigmentosa. Altered ARL2BP interactions affect proteins such as ARL2 and TBCE which contribute to disease pathology. Additionally these alterations may correlate with metabolic syndromes due to mitochondrial dysregulation linking ARL2BP to broader metabolic and neurodegenerative diseases.
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1 product image
Sandwich ELISA - Human ARL2BP ELISA Kit (ab283981)
Example of Human ARL2BP standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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