Human ARP4 ELISA Kit (Angiopoietin-Like 4) is a Sandwich (quantitative) ELISA kit for the measurement of Human ARP4 (Angiopoietin-Like 4) in Human in Plasma, Cell culture supernatant, Serum samples.
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Publications
- BMC infectious diseases 23:6772023A nomogram based on the expression level of angiopoietin-like 4 to predict the severity of community-acquired pneumonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiqin Chen,Jia Jiang,Minhong Su,Ping Chen,Xiang Liu,Wei Lei,Shaofeng Zhang,Qiang Wu,Fu Rong,Xi Li,Xiaobin Zheng,Qiang XiaoPubMed 37821811
- Oncology letters 24:2992022Association of high cellular expression and plasma concentration of angiopoietin-like 4 with tongue cancer lung metastasis and poor prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuya Tanaka,Takahisa Imamura,Atsushi Irie,Masakazu Yoneda,Ryuji Imamura,Ken Kikuchi,Saki Kitagawa,Tatsuko Kubo,Hidenao Ogi,Hideki NakayamaPubMed 35949602
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
27.4 - 20000 pg/mL
- Sensitivity
< 20 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed:17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity).ANGPTL4 N-terminal chainMediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed:19270337, PubMed:21398697).
Alternative names
Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein, HFARP, PGAR, ANGPTL4, ARP4, HFARP, PP1158, PSEC0166, UNQ171/PRO197
What's included?
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Human ARP4 ELISA Kit (Angiopoietin-Like 4) is a Sandwich (quantitative) ELISA kit for the measurement of Human ARP4 (Angiopoietin-Like 4) in Human in Plasma, Cell culture supernatant, Serum samples.
Alternative names
Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein, HFARP, PGAR, ANGPTL4, ARP4, HFARP, PP1158, PSEC0166, UNQ171/PRO197
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
27.4 - 20000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 20 pg/mL
- General recovery
100.4%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 117.8 | Range 67 - 137 % |
Sample type Serum | Average % = 95.38 | Range 85 - 104 % |
Sample type Plasma | Average % = 88.04 | Range 74 - 103 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's ARP4 (Angiopoietin-like 4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ARP4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human ARP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ARP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
Supplementary info
- Activity summary
The ARP4 protein also known as Actin-Related Protein 4 plays a role in chromatin remodeling which is important for DNA-related processes like transcription replication and repair. ARP4 has a molecular mass of approximately 50 kDa and is ubiquitously expressed in eukaryotic cells. It remains localized mainly within the nucleus where it contributes to changes in the configuration of chromatin structure permitting the necessary access of various transcription machinery to DNA.
- Biological function summary
ARP4 functions as an integral component of the INO80 chromatin remodeling complex. This complex is essential for modifying chromatin structure which impacts gene expression regulation and DNA repair. Through its association with the INO80 complex ARP4 contributes to the mobilization and spacing of nucleosomes important for maintaining genomic stability. It also further enhances DNA repair processes by allowing more accessible interactions with DNA sites requiring repair.
- Pathways
ARP4 is involved in the regulation of gene expression and DNA damage response. The protein plays a part in the chromatin remodeling pathway and the DNA repair pathway. Through its work with the INO80 complex ARP4 associates with other remodelers like RUVBL1 and RUVBL2 which are also components of the same complex. These interactions facilitate effective transcriptional regulation and the DNA damage response showcasing the critical nature of ARP4 in maintaining normal cellular functions.
- Associated diseases and disorders
ARP4 has a place in connecting with conditions like cancer and neurodegenerative diseases. Aberrant expression or mutations in ARP4 and its associated complexes can disrupt chromatin structure leading to misregulation of gene expression which can precipitate tumorigenesis or neuronal damage. Through its impact on chromatin remodeling and gene regulation ARP4 works alongside other proteins like BRG1 a part of the SWI/SNF chromatin remodeling complex influencing pathways that may result in these diseases.
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2 product images
Sandwich ELISA - Human ARP4 ELISA Kit (Angiopoietin-Like 4) (ab99974)
Representative standard curve using ab99974.
Sandwich ELISA - Human ARP4 ELISA Kit (Angiopoietin-Like 4) (ab99974)
Representative standard curve using ab99974.
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