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AB99974

Human ARP4 ELISA Kit (Angiopoietin-Like 4)

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(10 Publications)

Human ARP4 ELISA Kit (Angiopoietin-Like 4) is a Sandwich (quantitative) ELISA for the measurement of Human ARP4 (Angiopoietin-Like 4) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

ARP4, HFARP, PGAR, PP1158, PSEC0166, UNQ171/PRO197, ANGPTL4, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein

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Sandwich ELISA - Human ARP4 ELISA Kit (Angiopoietin-Like 4) (AB99974)
  • sELISA

Supplier Data

Sandwich ELISA - Human ARP4 ELISA Kit (Angiopoietin-Like 4) (AB99974)

Representative standard curve using ab99974.

Sandwich ELISA - Human ARP4 ELISA Kit (Angiopoietin-Like 4) (AB99974)
  • sELISA

Supplier Data

Sandwich ELISA - Human ARP4 ELISA Kit (Angiopoietin-Like 4) (AB99974)

Representative standard curve using ab99974.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

27.4 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Abcam's ARP4 (Angiopoietin-like 4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ARP4 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human ARP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ARP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "67 - 137 %", "average": "= 117.8" }, { "sample": "Serum", "range": "85 - 104 %", "average": "= 95.38" }, { "sample": "Plasma", "range": "74 - 103 %", "average": "= 88.04" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99974-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99974-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99974-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human ARP4", "number":"AB99974-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant ARP4 Standard Human (Lyophilized)", "number":"AB99974-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ARP4 Microplate (12 x 8 well strips)", "number":"AB99974-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"280X HRP-Streptavidin Concentrate", "number":"AB99974-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99974-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99974-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99974-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARP4 protein also known as Actin-Related Protein 4 plays a role in chromatin remodeling which is important for DNA-related processes like transcription replication and repair. ARP4 has a molecular mass of approximately 50 kDa and is ubiquitously expressed in eukaryotic cells. It remains localized mainly within the nucleus where it contributes to changes in the configuration of chromatin structure permitting the necessary access of various transcription machinery to DNA.
Biological function summary

ARP4 functions as an integral component of the INO80 chromatin remodeling complex. This complex is essential for modifying chromatin structure which impacts gene expression regulation and DNA repair. Through its association with the INO80 complex ARP4 contributes to the mobilization and spacing of nucleosomes important for maintaining genomic stability. It also further enhances DNA repair processes by allowing more accessible interactions with DNA sites requiring repair.

Pathways

ARP4 is involved in the regulation of gene expression and DNA damage response. The protein plays a part in the chromatin remodeling pathway and the DNA repair pathway. Through its work with the INO80 complex ARP4 associates with other remodelers like RUVBL1 and RUVBL2 which are also components of the same complex. These interactions facilitate effective transcriptional regulation and the DNA damage response showcasing the critical nature of ARP4 in maintaining normal cellular functions.

ARP4 has a place in connecting with conditions like cancer and neurodegenerative diseases. Aberrant expression or mutations in ARP4 and its associated complexes can disrupt chromatin structure leading to misregulation of gene expression which can precipitate tumorigenesis or neuronal damage. Through its impact on chromatin remodeling and gene regulation ARP4 works alongside other proteins like BRG1 a part of the SWI/SNF chromatin remodeling complex influencing pathways that may result in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 19270337, PubMed : 21398697, PubMed : 27929370, PubMed : 29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed : 14583458, PubMed : 17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed : 17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity).. ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 19270337, PubMed : 21398697, PubMed : 27929370, PubMed : 29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed : 19270337, PubMed : 21398697).
See full target information ANGPTL4
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

BMC infectious diseases 23:677 PubMed37821811

2023

A nomogram based on the expression level of angiopoietin-like 4 to predict the severity of community-acquired pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Siqin Chen,Jia Jiang,Minhong Su,Ping Chen,Xiang Liu,Wei Lei,Shaofeng Zhang,Qiang Wu,Fu Rong,Xi Li,Xiaobin Zheng,Qiang Xiao

Oncology letters 24:299 PubMed35949602

2022

Association of high cellular expression and plasma concentration of angiopoietin-like 4 with tongue cancer lung metastasis and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Tanaka,Takahisa Imamura,Atsushi Irie,Masakazu Yoneda,Ryuji Imamura,Ken Kikuchi,Saki Kitagawa,Tatsuko Kubo,Hidenao Ogi,Hideki Nakayama

Cell death & disease 13:272 PubMed35347106

2022

Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Dong Wang,Xiao-Jun Yu,Ji-Chun Hou,Bo Fu,Hao Zheng,Qi-Kun Liu,Shan-Xi Wang,Zheng-Gang Bi,Yang Cao

Frontiers in endocrinology 12:636272 PubMed33815293

2021

Gut Microbiota of Chinese Obese Children and Adolescents With and Without Insulin Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yuan,Ruimin Chen,Ying Zhang,Xiangquan Lin,Xiaohong Yang,Kenneth L McCormick

Cancer management and research 12:10521-10530 PubMed33122950

2020

LncRNA CCAL Promotes Angiogenesis Through Regulating the MiR-29b/ANGPTL4 Axis in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyi Chen,Mingjia Yang,Shimin Chang

World journal of pediatrics : WJP 15:565-571 PubMed31598832

2019

Adropin and glucagon-like peptide-2 are associated with glucose metabolism in obese children.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Min Chen,Xin Yuan,Qian Ouyang,Xiang-Quan Lin,Zhuan-Zhuan Ai,Ying Zhang,Xiao-Hong Yang

Journal of immunoassay & immunochemistry 39:84-98 PubMed29309212

2018

Development of bead based multiplexed immunoassay for evaluation of midkine, syndecan-1, and ANGPTL4 in patient serum.

Applications

Unspecified application

Species

Unspecified reactive species

Imad Tarhoni,Cristina L Fhied,Mark Pool,Michael J Liptay,Philip Bonomi,Christopher W Seder,Jeffrey A Borgia

Journal of immunology (Baltimore, Md. : 1950) 195:3605-16 PubMed26363050

2015

α1-Antitrypsin Combines with Plasma Fatty Acids and Induces Angiopoietin-like Protein 4 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Eileen Frenzel,Sabine Wrenger,Britta Brügger,Sandeep Salipalli,Stephan Immenschuh,Nupur Aggarwal,Ralf Lichtinghagen,Ravi Mahadeva,A Mario Q Marcondes,Charles A Dinarello,Tobias Welte,Sabina Janciauskiene

Applied microbiology and biotechnology 98:7549-57 PubMed24878750

2014

Clostridium butyricum reduce lipogenesis through bacterial wall components and butyrate.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhao,Yuming Guo,Hongbin Liu,Jing Gao,Wei Nie

PloS one 8:e73146 PubMed24039874

2013

c-Myc is essential to prevent endothelial pro-inflammatory senescent phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Florea,Nithya Bhagavatula,Gordana Simovic,Francisco Y Macedo,Ricardo A Fock,Claudia O Rodrigues
View all publications
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