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AB283538

Human Artemin ELISA Kit

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Human Artemin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Artemin with a sensitivity of 1.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

EVN, ARTN, Artemin, Enovin, Neublastin

3 Images
Sandwich ELISA - Human Artemin ELISA Kit (AB283538)
  • sELISA

Supplier Data

Sandwich ELISA - Human Artemin ELISA Kit (AB283538)

Interpolated concentrations of native Artemin in human A431 cell supernatant.

The concentrations of Artemin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : A431 cell supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 37.5 pg/mL in A431 cell supernatant.

Sandwich ELISA - Human Artemin ELISA Kit (AB283538)
  • sELISA

Supplier Data

Sandwich ELISA - Human Artemin ELISA Kit (AB283538)

Serum from eight individual healthy Artemin female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Seven samples tested below the standard curve. The detectable sample had an Artemin concentration of 8.0 pg/mL.

Sandwich ELISA - Human Artemin ELISA Kit (AB283538)
  • sELISA

Supplier Data

Sandwich ELISA - Human Artemin ELISA Kit (AB283538)

Example of human Artemin standard curve in Sample Diluent NS.

The Artemin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.5 pg/mL

Range

4.7 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Artemin ELISA kit (ab283538) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Artemin protein in human serum and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Artemin with 1.5 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Artemin, also known as enovin or neublastin, is a protein that is encoded by the ARTN gene. ARTN is a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta superfamily of proteins. Artemin serves as a ligand for the GFR-alpha-3-RET receptor complex and can activate the GFR-alpha-1-RET receptor complex. The antibodies in this product were raised to the mature sequence of human Artemin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "114 - 119 %", "average": "= 117" }, { "sample": "Serum", "range": "117 - 120 %", "average": "= 118" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283538-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283538-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283538-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283538-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283538-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283538-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283538-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB283538-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Artemin Capture Antibody", "number":"AB283538-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Artemin Lyophilized Recombinant Protein", "number":"AB283538-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Artemin Detector Antibody", "number":"AB283538-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Artemin also known as ART or ARTN is a neurotrophic factor and part of the glial cell line-derived neurotrophic factor (GDNF) family. It comprises a mature peptide with a molecular mass of approximately 24 kDa. This protein expresses highly in the peripheral and central nervous system particularly in developing and injured tissues. Artemin supports the survival and differentiation of specific neurons contributing to neurite outgrowth and neuronal regeneration.
Biological function summary

Artemin is a significant modulator of neuronal survival and neurite extension. It forms a signaling complex with its receptor components the GDNF family receptor alpha 3 (GFRα3) and the receptor tyrosine kinase (RET). This complex initiates signaling cascades essential for embryonic neuronal development and adult neuroprotection. Artemin selectively activates certain pathways that promote neuronal survival and regenerative responses.

Pathways

Artemin engages with the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/AKT) and the mitogen-activated protein kinase (MAPK/ERK) pathways. These pathways are critical for cell survival proliferation and differentiation. Artemin closely associates with other neurotrophic factors such as GDNF within these pathways suggesting a shared or complementary role in neuroprotection.

Artemin shows potential involvement in neurodegenerative diseases and peripheral neuropathies. It may have therapeutic implications in conditions such as Parkinson's disease where it enhances neuronal survival. Similarly its role in peripheral neuropathy suggests that it modulates pain through interactions with receptors like GFRα3 possibly impacting RET signaling pathways.
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Product protocols

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Target data

Growth factor that supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain (PubMed : 10583383, PubMed : 9883723). Acts by binding to its coreceptor, GFRA3, leading to autophosphorylation and activation of the RET receptor (PubMed : 31535977). Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity).
See full target information ARTN
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websiteProtocolBooklet
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Product promise

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