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AB313510

Human ASAH2 ELISA Kit

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Human ASAH2 ELISA Kit is a Sandwich ELISA for the measurement of Human ASAH2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

HNAC1, ASAH2, Neutral ceramidase, N-CDase, NCDase, Acylsphingosine deacylase 2, BCDase, LCDase, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, hCD

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Sandwich ELISA - Human ASAH2 ELISA Kit (AB313510)
  • sELISA

Supplier Data

Sandwich ELISA - Human ASAH2 ELISA Kit (AB313510)

Example of standard curve using ab313510. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.41 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ASAH2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ASAH2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ASAH2 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, NKp46/NCR1, Neuronal Pentraxin R/NPTXR, ROR2, SCCA2, Siglec-2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3.

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Recovery

[ { "sample": "Cell culture media", "range": "109 - 116 %", "average": "= 112.9" }, { "sample": "Plasma", "range": "106 - 124 %", "average": "= 113.3" }, { "sample": "Serum", "range": "98 - 116 %", "average": "= 109.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASAH2 also known as N-acylsphingosine amidohydrolase 2 is an important enzyme responsible for the hydrolysis of ceramides into sphingosine and free fatty acids. It has a molecular mass of approximately 85 kDa. ASAH2 is expressed in many tissues with prominent expression observed in the small intestine and brain. Its activity influences cell membrane integrity and intercellular communication which plays an essential role in the normal functioning of cells.
Biological function summary

ASAH2 regulates the levels of ceramide and sphingosine both of which are important lipid signaling molecules. This enzyme exists within cells as part of a complex that maintains lipid homeostasis impacting numerous biological processes through ceramide metabolism. The balance between ceramide and sphingosine signaling is important for cell survival proliferation and apoptosis highlighting the importance of ASAH2 in these cellular dynamics.

Pathways

ASAH2 involves itself in the sphingolipid metabolism pathway which is significant for signal transduction and cellular communication. ASAH2 works closely with proteins like sphingosine kinase which further metabolizes sphingosine into sphingosine-1-phosphate (S1P) a lipid mediator with diverse functions. This pathway plays essential roles in inflammation immune response and cell growth demonstrating the interconnected nature of ASAH2's activity with other critical proteins.

ASAH2 has associations with metabolic disorders and neurodegenerative diseases. Elevated ceramide levels due to ASAH2 dysfunction relate to disorders such as Gaucher disease characterized by abnormal lipid storage and may influence neurodegenerative conditions like Alzheimer's disease. The enzyme interacts with proteins like acid ceramidase where imbalances in these enzymes lead to disruptions in lipid metabolism and contribute to disease pathology making ASAH2 a potential target for therapeutic intervention.
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Product protocols

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Target data

Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed : 10781606, PubMed : 16229686, PubMed : 26190575). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed : 15946935, PubMed : 19345744, PubMed : 24798654). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed : 11278489, PubMed : 17475390). Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed : 16061940). Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).
See full target information ASAH2
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Product promise

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