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AB309406

Human ASGR2 ELISA Kit

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Human ASGR2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ASGR2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CLEC4H2, ASGR2, Asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2

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Sandwich ELISA - Human ASGR2 ELISA Kit (AB309406)
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Supplier Data

Sandwich ELISA - Human ASGR2 ELISA Kit (AB309406)

Example of standard curve using ab309406. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.15 ng/mL

Range

0.15 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ASGR2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human ASGR2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "70 - 103 %", "average": "= 91.2" }, { "sample": "Plasma", "range": "70 - 97 %", "average": "= 83.15" }, { "sample": "Cell culture supernatant", "range": "70 - 88 %", "average": "= 76.91" }, { "sample": "Cell culture media", "range": "70 - 88 %", "average": "= 76.91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309406-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309406-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309406-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ASGP-R 2/ASGR2 Antibody-coated ELISA Plate", "number":"AB309406-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ASGP-R 2/ASGR2 Protein Standard", "number":"AB309406-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ASGP-R 2/ASGR2 Detection Antibody", "number":"AB309406-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309406-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309406-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB309406-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The asialoglycoprotein receptor 2 (ASGR2) sometimes called Ashwell receptor subunit 2 is a transmembrane protein with a mass of approximately 46 kDa. ASGR2 combines with a similar protein ASGR1 to form a functional receptor complex. It is mostly found in the liver playing an important role in endocytosis by binding galactose-terminated glycoproteins. The receptor facilitates the clearance of these glycoproteins from the bloodstream making the liver a major site for their catabolism.
Biological function summary

ASGR2 participates in regulating serum glycoprotein levels. It is part of the asialoglycoprotein receptor complex working with ASGR1 to internalize glycoproteins for lysosomal degradation. This process is important for maintaining homeostasis of serum proteins and influencing liver function. Its activity affects hepatocyte turnover and cellular response to extracellular stimuli within the liver.

Pathways

ASGR2 is involved in the endocytosis and trafficking pathways. These pathways guide the internalization and lysosomal degradation of serum glycoproteins. ASGR2's interaction with ASGR1 through these pathways highlights its role in glycoprotein metabolism. Additionally its function intersects with the complement system's mannose-binding protein affecting immune response by clearing immune complexes.

ASGR2's dysfunction correlates with liver conditions like congenital disorders of glycosylation and hepatitis B virus infection. In hepatitis B altered ASGR2 function affects virus binding and clearance. The asialoglycoprotein receptor complex including the ASGR1/ASGR2 interaction is central to understanding changes in liver pathology and therapeutic targeting of liver diseases.
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Product protocols

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Target data

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
See full target information ASGR2
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Complementary Products

Primary Antibodies

AB216521

Anti-ASGR2 antibody [EPR16975] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

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Proteins & Peptides

AB134625

Recombinant Human ASGR2 protein

SDS-PAGE - Recombinant Human ASGR2 protein (AB134625)

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