Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Asialoglycoprotein Receptor 1/HL-1 in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.22 - 60 ng/mL
- Sensitivity
- = 0.22 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
Alternative names
CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1
What's included?
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Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Asialoglycoprotein Receptor 1/HL-1 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.22 - 60 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.22 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 129.3 | Range 105 - 139 % |
Sample type Plasma | Average % = 89.68 | Range 74 - 115 % |
Sample type Cell culture media | Average % = 118.9 | Range 102 - 133 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Asialoglycoprotein Receptor 1/HL-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Asialoglycoprotein Receptor 1/HL-1 coated on a 96-well plate
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.
- Biological function summary
The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.
- Pathways
ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.
- Associated diseases and disorders
ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.
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1 product image
Sandwich ELISA - Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit (ab309431)
Example of standard curve using ab309431. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
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