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AB309431

Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit

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Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human Asialoglycoprotein Receptor 1/HL-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

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Sandwich ELISA - Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit (AB309431)
  • sELISA

Supplier Data

Sandwich ELISA - Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit (AB309431)

Example of standard curve using ab309431. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.22 ng/mL

Range

0.22 - 60 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Asialoglycoprotein Receptor 1/HL-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Asialoglycoprotein Receptor 1/HL-1 coated on a 96-well plate
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Recovery

[ { "sample": "Serum", "range": "105 - 139 %", "average": "= 129.3" }, { "sample": "Plasma", "range": "74 - 115 %", "average": "= 89.68" }, { "sample": "Cell culture media", "range": "102 - 133 %", "average": "= 118.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309431-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309431-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB309431-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ASGP-R 1/ASGR1 Detection Antibody", "number":"AB309431-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309431-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309431-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309431-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309431-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ASGP-R 1/ASGR1 Antibody-coated ELISA Plate", "number":"AB309431-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ASGP-R 1/ASGR1 Protein Standard", "number":"AB309431-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.
Biological function summary

The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.

Pathways

ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.

ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.
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Product protocols

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Target data

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
See full target information ASGR1
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Complementary Products

Primary Antibodies

AB254262

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (AB254262)

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Proteins & Peptides

AB151893

Recombinant Human Asialoglycoprotein Receptor 1/HL-1

SDS-PAGE - Recombinant Human Asialoglycoprotein Receptor 1/HL-1 (AB151893)

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