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AB325905

Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent

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Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human AST (Aspartate Aminotransferase) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 82.3 - 60000 pg/ml with a sensitivity of 19.94 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

7 Images
Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)
  • sELISA

Lab

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)

Interpolated concentration of native AST was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)
  • sELISA

Lab

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)

Standard curve comparison between the Chemiluminescent Human AST SimpleStep ELISA and colorimetric Human AST SimpleStep ELISA (ab263881) in Sample Diluent 25BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)
  • sELISA

Lab

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)

Serum of ten individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58.1 ng/mL with a range of 26.6 135.6 ng/mL.

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)
  • sELISA

Lab

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)

Dynamic range comparison between the Chemiluminescent Human AST SimpleStep ELISA, colorimetric Human AST SimpleStep ELISA (ab263881), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)
  • sELISA

Lab

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)

Interpolated concentration of native AST was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Human Liver Tissue Extract 10 ug/mL and HepG2 Cell Extract 75 ug/mL. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)
  • sELISA

Lab

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)

Interpolated concentration of native AST was measured in duplicate at different sample concentrations. Undiluted samples are 50% HepG2 Cell Culture Supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)
  • sELISA

Lab

Sandwich ELISA - Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (AB325905)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cell Extract, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 19.94 pg/mL

Range

82.3 - 60000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (ab325905) is a rapid single-wash sandwich 55-min ELISA to measure Human AST (Aspartate Aminotransferase) in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Cell Extract, Tissue Extract with a sensitivity of 19.94 pg/mL.

How the assay works

Human AST (Aspartate Aminotransferase) ELISA Kit, Chemiluminescent (ab325905) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human AST (Aspartate Aminotransferase) (ab325905) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GOT1 (aspartate transaminase 1) antibody [EPR20781-274] - BSA and Aside free (Capture) ab259691
- Anti-GOT1 (aspartate transaminase 1) antibody [EPR20781-282] - BSA and Aside free (Detector) ab259692

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.8" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 114 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "89 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "91 - 96 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "101 - 104 %", "average": "= 103" }, { "sample": "Cell culture supernatant", "range": "81 - 109 %", "average": "= 93" }, { "sample": "Cell Lysate", "range": "97 - 103 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "88 - 94 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).
See full target information Aspartate aminotransferase, cytoplasmic
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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