Human AST ELISA Kit , 90-min ELISA (ab263881)

Human AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min Simplestep used to quantify Human AST (Aspartate Aminotransferase) with a sensitivity of 43 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881), expandable thumbnail

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Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 312.5 - 20000 pg/mL
Assay time: 1h 30m
Sensitivity: = 43 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information Aspartate aminotransferase, cytoplasmic

Function

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456).

Alternative names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

What's included?

1 x 384 Tests

Components

10X Human AST (Aspartate Aminotransferase) Capture Antibody

1 x 600 µL

10X Human AST (Aspartate Aminotransferase) Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

50X Cell Extraction Enhancer Solution (ab193971)

6 x 1 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent 4BI

1 x 6 mL

Human AST (Aspartate Aminotransferase) Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent 25BS

1 x 20 mL

Sample Diluent NS (ab193972)

1 x 50 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 312.5 - 20000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 43 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Serum	n 8	C.V. 4.2

Inter assay

Sample	n	C.V.
Sample Serum	n 3	C.V. 1.8

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture extracts	Average % = 92	Range 88 - 94 %
Sample type Cell culture supernatant	Average % = 93	Range 81 - 109 %
Sample type Citrate plasma	Average % = 93	Range 89 - 97 %
Sample type EDTA Plasma	Average % = 93	Range 91 - 96 %
Sample type Heparin Plasma	Average % = 103	Range 101 - 104 %
Sample type Serum	Average % = 101	Range 95 - 114 %
Sample type Tissue Extracts	Average % = 99	Range 97 - 103 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 is a rapid single-wash 90-min ELISA kit to measure Human AST in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 43 pg/mL.

How the assay works

Human AST SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed for the quantification of human AST. The standard protein in this kit is full length human AST. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), cell culture supernatant, cell extract and tissue extract sample types. Saliva, urine, milk, and CSF samples have not been tested with this kit.

Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human AST ELISA Kit (Aspartate Aminotransferase) ab263881

Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 has been used to study oxidative and inflammatory responses to smoking ⁽¹⁾, blood biomarker expression in acute pancreatitis ⁽²⁾, and human Liver-Chip as a model for predicting drug-induced liver injury ⁽³⁾.

References:

⁽¹⁾Hande Sipahi et al. 2023, PMID: 36650104,
⁽²⁾Huaming Ding et al. 2022, PMID: 36816737,
⁽³⁾Janey Ronxhi, et al. 2019, PMID: 35790095

Save your precious samples

Our 384-well Human AST ELISA Kit (Aspartate Aminotransferase) only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free Human/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free ab267701
- Anti-Aspartate Aminotransferase antibody [EPR20781-274] - BSA and Azide free (Capture) Anti-Aspartate Aminotransferase antibody [EPR20781-274] - BSA and Azide free (Capture) ab259691
- Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector) Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector) ab259692

Related and recommended products

Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 is commonly used by researchers to measure liver function.

Other ELISA kits to measure liver function include:

- Human ALT ELISA kit Human ALT ELISA Kit ab234578
- Human Albumin ELISA kit Human Albumin ELISA Kit ab179887
- Albumin (BCG) Assay Kit (Colorimetric) Albumin (BCG) Assay Kit (Colorimetric) ab235628

These ELISA kits are also available for Mouse/Rat:

- Mouse AST ELISA kit Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882
- Mouse ALT ELISA kit Mouse ALT ELISA Kit ab282882
- Mouse Albumin ELISA kit Mouse Albumin ELISA Kit ab207620
- Rat AST ELISA kit Rat AST ELISA Kit (Aspartate Aminotransferase) ab263883
- Rat ALT ELISA kit abab234579
- Rat Albumin ELISA kit Rat Albumin ELISA Kit ab235642

Cytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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17 product images

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentrations of native AST in human serum and plasma samples.
The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 62.7 ng/mL in serum, 38.7 ng/mL in plasma (citrate), 51.9 ng/mL in plasma (EDTA). and 41.0 pg/mL in plasma (heparin).
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentrations of native AST in human liver extract based on a 10 μg/mL extract load.
The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 13.5 ng/mL in human liver extract.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58.1 ng/mL with a range of 26.6 – 135.6 ng/mL.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Example of human AST standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Example of human AST standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58 ng/mL with a range of 27 – 136 ng/mL.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentrations of native AST in human liver extract and HepG2 cell extract samples based on 10 and 75 μg/mL extract loads, respectively.
The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 14 ng/mL in human liver extract and 14 ng/mL in HepG2 cell extract.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentrations of native AST in HepG2 cell culture supernatant samples.
The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 13 ng/mL in HepG2 cell culture supernatant.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentrations of native AST in human serum and plasma samples.
The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 63 ng/mL in serum, 39 ng/mL in plasma (citrate), 52 ng/mL in plasma (EDTA). and 41 ng/mL in plasma (heparin).
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Example of human AST standard curve in 1X Cell Extraction Buffer PTR.
The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Example of human AST standard curve in Sample Diluent NS.
The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Example of human AST standard curve in Sample Diluent 25BS.
The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL HepG2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Example of human AST standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.