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AB263881

Human AST ELISA Kit (Aspartate Aminotransferase)

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(5 Publications)

Human AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min Simplestep used to quantify Human AST (Aspartate Aminotransferase) with a sensitivity of 43 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

18 Images
ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • ELISA

Supplier Data

ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Lab

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of human AST in serum in 96-well vs. 384-well plates

Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Lab

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of human AST in HepG2 cell extract in 96-well vs. 384-well plates

Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL HepG2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Lab

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of human AST in HepG2 supernatant in 96-well vs. 384-well plates

Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Lab

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Example of human AST standard curve in Sample Diluent 25BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Lab

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Example of human AST standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Lab

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Example of human AST standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Example of human AST standard curve in 1X Cell Extraction Buffer PTR.

The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of native AST in human liver extract based on a 10 μg/mL extract load.

The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 13.5 ng/mL in human liver extract.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Example of human AST standard curve in Sample Diluent NS.

The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of native AST in human liver extract and HepG2 cell extract samples based on 10 and 75 μg/mL extract loads, respectively.

The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 14 ng/mL in human liver extract and 14 ng/mL in HepG2 cell extract.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of native AST in HepG2 cell culture supernatant samples.

The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 13 ng/mL in HepG2 cell culture supernatant.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of native AST in human serum and plasma samples.

The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 62.7 ng/mL in serum, 38.7 ng/mL in plasma (citrate), 51.9 ng/mL in plasma (EDTA). and 41.0 pg/mL in plasma (heparin).

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Interpolated concentrations of native AST in human serum and plasma samples.

The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 63 ng/mL in serum, 39 ng/mL in plasma (citrate), 52 ng/mL in plasma (EDTA). and 41 ng/mL in plasma (heparin).

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58 ng/mL with a range of 27 – 136 ng/mL.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58.1 ng/mL with a range of 26.6 – 135.6 ng/mL.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

Example of human AST standard curve in Sample Diluent 25BS.

The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)
  • sELISA

Supplier Data

Sandwich ELISA - Human AST ELISA Kit (Aspartate Aminotransferase) (AB263881)

The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 43 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 is a rapid single-wash 90-min ELISA kit to measure Human AST in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 43 pg/mL.

How the assay works
Human AST SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit is designed for the quantification of human AST. The standard protein in this kit is full length human AST. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), cell culture supernatant, cell extract and tissue extract sample types. Saliva, urine, milk, and CSF samples have not been tested with this kit.

Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 has been used to study oxidative and inflammatory responses to smoking (1), blood biomarker expression in acute pancreatitis (2), and human Liver-Chip as a model for predicting drug-induced liver injury (3).

References:
(1)Hande Sipahi et al. 2023, PMID: 36650104,
(2)Huaming Ding et al. 2022, PMID: 36816737,
(3)Janey Ronxhi, et al. 2019, PMID: 35790095

Save your precious samples
Our 384-well Human AST ELISA Kit (Aspartate Aminotransferase) only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free ab267701
- Anti-Aspartate Aminotransferase antibody [EPR20781-274] - BSA and Azide free (Capture) ab259691
- Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector) ab259692

Related and recommended products
Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 is commonly used by researchers to measure liver function.

Other ELISA kits to measure liver function include:
- Human ALT ELISA kit ab234578
- Human Albumin ELISA kit ab179887
- Albumin (BCG) Assay Kit (Colorimetric) ab235628

These ELISA kits are also available for Mouse/Rat:
- Mouse AST ELISA kit ab263882
- Mouse ALT ELISA kit ab282882
- Mouse Albumin ELISA kit ab207620
- Rat AST ELISA kit ab263883
- Rat ALT ELISA kit ab234579
- Rat Albumin ELISA kit ab235642

Cytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "88 - 94 %", "average": "= 92" }, { "sample": "Cell culture supernatant", "range": "81 - 109 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "89 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "91 - 96 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "101 - 104 %", "average": "= 103" }, { "sample": "Serum", "range": "95 - 114 %", "average": "= 101" }, { "sample": "Tissue Extracts", "range": "97 - 103 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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Product protocols

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Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).
See full target information Aspartate aminotransferase, cytoplasmic
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in toxicology 7:1574387 PubMed40575028

2025

Bioengineering of novel organotypic 3D human liver tissue model for drug-induced liver injury and toxicity studies.

Applications

Unspecified application

Species

Unspecified reactive species

Camden Holm,Joseph Finelli,Mateo Frare,Alex Armento,Seyoum Ayehunie

Current research in food science 9:100919 PubMed39582575

2024

Ultrasonication coupled to enzymatic hydrolysis of soybean okara proteins for producing bioactive and bioavailable peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenza d'Adduzio,Melissa Fanzaga,Anna Laura Capriotti,Enrico Taglioni,Giovanna Boschin,Aldo Laganà,Lukas Rueller,Josef Robert,Antje van Gemmern,Carlotta Bollati,Carmen Lammi

Chemistry & biodiversity 20:e202200928 PubMed36650104

2023

Alpha-Lipoic Acid Modulates the Oxidative and Inflammatory Responses Induced by Traditional and Novel Tobacco Products in Human Liver Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rengin Reis,Kübra Kolci,İrem Bahcivan,Goknil Pelin Coskun,Hande Sipahi

Annals of surgical treatment and research 104:90-100 PubMed36816737

2023

Expression levels of and in peripheral blood of severe acute pancreatitis and their clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyong Liang,Huaming Ding

Current protocols 2:e478 PubMed35790095

2022

Co-Culture of Human Primary Hepatocytes and Nonparenchymal Liver Cells in the Emulate® Liver-Chip for the Study of Drug-Induced Liver Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Shi,Ayesha Arefin,Lijun Ren,Katy S Papineau,Dustyn A Barnette,Laura K Schnackenberg,Jessica J Hawes,Mark Avigan,Donna L Mendrick,Lorna Ewart,Janey Ronxhi
View all publications
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Product promise

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