AB288169

Human ATG7 ELISA Kit

Recombinant
SimpleStep
What is this?

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Human ATG7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ATG7 with a sensitivity of 58.04 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

APG7L, ATG7, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein, APG7-like, hAGP7

3 Images

Sandwich ELISA - Human ATG7 ELISA Kit (AB288169)

sELISA

Supplier Data

Sandwich ELISA - Human ATG7 ELISA Kit (AB288169)

Interpolated concentrations of human ATG7 in stomach tissue extract and HeLa cell extract.

Interpolated concentration of native ATG7 was measured in duplicate at different sample concentrations. Undiluted samples are 200 μg/mL stomach tissue extract and HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human ATG7 ELISA Kit (AB288169)

Example of human ATG7 standard curve in 1X Cell Extraction buffer PTR. Example of human ATG7 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human ATG7 ELISA Kit (AB288169)

Example of human ATG7 standard curve in Sample Diluent NS. Example of human ATG7 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 58.04 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ATG7 ELISA Kit (ab288169) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ATG7 protein in human cell lysate and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate ATG7 with 58.041 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 117 %", "average": "= 107" }, { "sample": "Cell Lysate", "range": "97 - 114 %", "average": "= 104" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288169-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288169-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB288169-CMP15", "productcode":"" }, { "size":"1 x 600 µL", "name":"Human ATG7 Lyophilized Recombinant Protein", "number":"AB288169-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288169-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288169-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ATG7 Capture Antibody", "number":"AB288169-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288169-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB288169-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288169-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB288169-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288169-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human ATG7 Detector Antibody", "number":"AB288169-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG7 also known as Autophagy Related 7 is an essential protein involved in the autophagy process. It functions as an E1-like enzyme activating and transferring ubiquitin-like proteins such as ATG12 and LC3. The molecular weight of ATG7 is approximately 78 kDa. It is widely expressed in various tissues although expression levels can differ. One can detect ATG7 using immunoassays like ELISA or antibodies specifically targeting ATG7. Understanding its mechanical role is key to studying cellular homeostasis.

Biological function summary

ATG7 plays a significant role in autophagy a cellular degradation pathway critical for cell survival under stress. ATG7 contributes to the formation of autophagosomes by facilitating conjugation of ATG8 family proteins including LC3 to phosphatidylethanolamine. It acts within complexes that regulate cellular energy balance and stress responses ensuring cells maintain their function and integrity. Knockdown of ATG7 can impair autophagic flux highlighting its importance in maintaining cellular processes.

Pathways

ATG7 is a central player in the autophagy pathway influencing cellular metabolism and turnover. It interacts closely with ATG5 and ATG12 in this pathway to form a conjugation system essential for autophagosome elongation. Additionally ATG7 is involved in the mTOR signaling pathway which regulates nutrient sensing and cellular growth. Interaction with proteins like mTOR allows ATG7 to integrate signals from nutrient availability and stress responses finely tuning the autophagy process.

ATG7 dysfunction has connections to cancer and neurodegenerative diseases like Alzheimer's. Abnormal ATG7 activity disrupts autophagic balance possibly leading to the accumulation of damaged proteins and organelles contributing to disease progression. In cancer altered ATG7 expression may influence tumor survival by affecting cellular stress responses. Proteins such as p53 involved in cell cycle regulation often show association with ATG7-related pathways indicating a complex network influencing disease states. Understanding ATG7's role in these conditions can help explore potential therapeutic strategies.

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Product protocols

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Target data

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes (PubMed : 34161705). Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).

See full target information ATG7

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human ATG7 ELISA Kit

Sandwich ELISA - Human ATG7 ELISA Kit (AB288169)

Sandwich ELISA - Human ATG7 ELISA Kit (AB288169)

Sandwich ELISA - Human ATG7 ELISA Kit (AB288169)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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