JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB270890

Human ATGL ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human ATGL ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ATGL with a sensitivity of 40.78 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ATGL, FP17548, PNPLA2, Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Calcium-independent phospholipase A2-zeta, Desnutrin, Pigment epithelium-derived factor receptor, TTS2.2, Transport-secretion protein 2, iPLA2-zeta, PEDF-R, TTS2

3 Images
Sandwich ELISA - Human ATGL ELISA Kit (AB270890)
  • sELISA

Supplier Data

Sandwich ELISA - Human ATGL ELISA Kit (AB270890)

Example of human ATGL standard curve in 1X Cell Extraction Buffer PTR.

The ATGL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ATGL ELISA Kit (AB270890)
  • sELISA

Supplier Data

Sandwich ELISA - Human ATGL ELISA Kit (AB270890)

Representative image of Human ATGL ELISA Kit (ab270890)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human ATGL ELISA Kit Capture Antibody

- 10X Human ATGL Detector Antibody

- 10X Human ATGL ELISA Kit Lyophilised Purified Protein (2 vials)

- Human ATGL ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human ATGL ELISA Kit (AB270890)
  • sELISA

Supplier Data

Sandwich ELISA - Human ATGL ELISA Kit (AB270890)

Interpolated concentrations of native ATGL in human HeLa cell extract based on a 1,500 μg/mL extract load.

The concentrations of ATGL were measured in duplicate and interpolated from the ATGL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ATGL concentration was determined to be 4572.12 pg/mL in HeLa cell extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 40.78 pg/mL

Range

218.75 - 14000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human ATGL ELISA kit (ab270890) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ATGL protein in human cell and tissue cullture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human ATGL with 40.78 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Adipose Triglyceride Lipase (ATGL) catalyzes the initial step in triglyceride hydrolysis in both adipocyte and non-adipocyte lipid droplets. ATGL regulates adiposome size and may be involved in the degradation of adiposomes. ATGL exhibits acylglycerol transaclase activity and may coordinate with LIPE/HLS within the lipolytic cascade. ATGL is also thought to play an important role in energy homeostasis and a role in the response of the organism to starvation. ATGL enhances the hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. ab270890 was designed against the luminal fragment of the ATGL protein. Human ATGL protein sequence identity as follows: mouse, rat, cow, monkey are 90%, 90%, 84% and 92%, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
style
left-column

Recovery

[ { "sample": "Cell culture extracts", "range": "82 - 93 %", "average": "= 86" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270890-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270890-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270890-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB270890-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270890-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270890-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270890-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ATGL Lyophilized Recombinant Protein", "number":"AB270890-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ATGL Capture Antibody", "number":"AB270890-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB270890-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB270890-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270890-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ATGL Detector Antibody", "number":"AB270890-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adipose Triglyceride Lipase often referred to as ATGL is an important enzyme in lipid metabolism. Also known as lipase 29 or desnutrin ATGL hydrolyzes triglycerides into diglycerides and free fatty acids the first step in the breakdown of stored fat. The molecular weight of the ATGL protein is approximately 54 kDa. Cells express ATGL abundantly in adipose tissues but it also appears in cardiac and skeletal muscles highlighting its importance in energy management across different tissues.
Biological function summary

ATGL is part of the lipid mobilization process and contributes significantly to energy homeostasis. It acts distinctively as it breaks down phosphotriglycerides and is not part of a larger complex carrying out its function independently. Its activity impacts the balance of lipid storage and release important for maintaining energy levels within cells and across the entire organism.

Pathways

ATGL plays a significant role in the lipid catabolism and energy regulation pathways. ATGL's activity regulates the fat mobilization pathway by interacting closely with the protein Comparative Gene Identification-58 (CGI-58) which activates it. Further ATGL is involved in the peroxisome proliferator-activated receptor (PPAR) pathway highlighting its role in influencing metabolic processes and energy balance.

ATGL dysregulation connects to metabolic disorders such as obesity and non-alcoholic fatty liver disease (NAFLD). Reduced ATGL activity leads to excessive triglyceride accumulation in adipose tissue contributing to the development of obesity. Moreover ATGL deficiencies can link to cardiac issues through its connection with another lipase Hormone-Sensitive Lipase (HSL) affecting lipid content in cardiac tissues and influencing cardiomyopathies. Understanding ATGL's precise function aids in the development of therapeutic strategies for these conditions.
style
left-column

Product protocols

style
left-column

Target data

Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed : 15364929, PubMed : 15550674, PubMed : 16150821, PubMed : 16239926, PubMed : 17603008, PubMed : 34903883). Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade (By similarity). Also possesses acylglycerol transacylase and phospholipase A2 activities (PubMed : 15364929, PubMed : 17032652, PubMed : 17603008). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (PubMed : 17603008). Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed : 16239926). Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes (PubMed : 35676490). Acts antagonistically with LDAH in regulation of cellular lipid stores (PubMed : 28578400). Inhibits LDAH-stimulated lipid droplet fusion (PubMed : 28578400). May play an important role in energy homeostasis (By similarity). May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion (By similarity).
See full target information PNPLA2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB178780

Triglyceride Assay Kit (Fluorometric, Reducing Samples)

Functional Studies - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (AB178780)

  • 3
  • 1
Assay Kits

AB65336

Triglyceride Assay Kit - Quantification

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

  • 4
  • 4
Primary Antibodies

AB207799

Anti-Adipose Triglyceride Lipase antibody [EPR19650]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (AB207799)

  • 0
  • 0
Proteins & Peptides

AB226233

Recombinant Human Adipose Triglyceride Lipase protein (His tag)

SDS-PAGE - Recombinant Human Adipose Triglyceride Lipase protein (His tag) (AB226233)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com