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AB309449

Human B4GALT1 ELISA Kit

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Human B4GALT1 ELISA Kit is a Sandwich ELISA for the measurement of Human B4GALT1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GGTB2, B4GALT1, Beta4Gal-T1, b4Gal-T1, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase

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Sandwich ELISA - Human B4GALT1 ELISA Kit (AB309449)
  • sELISA

Supplier Data

Sandwich ELISA - Human B4GALT1 ELISA Kit (AB309449)

Example of standard curve using ab309449. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.32 ng/mL

Range

0.32 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human B4GALT1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human B4GALT1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human B4GALT1 coated on a 96-well plate
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Recovery

[ { "sample": "Serum", "range": "76 - 145 %", "average": "= 124.4" }, { "sample": "Plasma", "range": "75 - 137 %", "average": "= 107" }, { "sample": "Cell culture supernatant", "range": "83 - 146 %", "average": "= 119.2" }, { "sample": "Cell culture media", "range": "83 - 146 %", "average": "= 119.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309449-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human B4GalT1 Protein Standard", "number":"AB309449-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309449-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309449-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human B4GalT1 Antibody-coated ELISA Plate", "number":"AB309449-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human B4GalT1 Detection Antibody", "number":"AB309449-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin", "number":"AB309449-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309449-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309449-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B4GALT1 also known as beta-14-galactosyltransferase 1 is an enzyme that facilitates the transfer of galactose residues from UDP-galactose to acceptor sugars forming β1-4 galactosidic linkages. This enzyme has an approximate mass of 60 kDa. It is primarily expressed in the Golgi apparatus of many cell types including epithelial cells and fibroblasts. B4GALT1 represents one of seven beta-14-galactosyltransferase enzymes each with varying specificity and function depending on the tissue type.
Biological function summary

B4GALT1 plays an important role in the modification of glycoproteins and glycolipids. It acts as part of a larger complex responsible for the formation and maintenance of glycan structures on cellular proteins which are important for cell-cell communication and protein stability. This modification process is essential for normal cellular functions because it impacts protein folding secretion and cell surface expression. B4GALT1 achieves this by participating in the synthesis of N-linked and O-linked glycans which directly influences various physiological processes.

Pathways

B4GALT1 functions in the glycosylation pathway where it modifies oligosaccharide chains during protein post-translational modification. This pathway is essential for the synthesis of glycoproteins which play a role in cell adhesion and signaling. B4GALT1 also contributes to the protein N-glycosylation metabolic pathway and interacts with proteins like ST3GAL1 which modify glycan structures further. These pathways are indispensable for proper cellular communication and function.

Defects or dysregulation of B4GALT1 can lead to conditions such as congenital disorders of glycosylation. Mutations in this enzyme have been linked to neurological and developmental abnormalities due to impaired glycan synthesis. B4GALT1 has also shown relevance in cancer influencing tumor progression and metastasis. It interacts with MUC1 a protein that when aberrantly glycosylated can promote cancer cell invasiveness and impede immune response therefore affecting cancer prognosis.
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Product protocols

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Target data

Galactosyltransferase acting in the Golgi stacks. Catalyzes the transfer of galactose (Gal) from UDP-alpha-D-galactose in beta(1->4) linkage to the non-reducing terminal N-acetylglucosamine (GlcNAc) moieties of glycolipids and complex-type N-linked glycans (PubMed : 16157350, PubMed : 27872474, PubMed : 29133956, PubMed : 36280670, PubMed : 37632720, PubMed : 38321209). Adds one Gal residue to both GlcNAc beta(1->2)-linked to the alpha(1->3) and alpha(1->6) mannose antennae of complex-type N-glycans, enabling the formation of mono- and di-galactosylated glycoforms. Galactosylates complex-type N-glycans attached on the fragment crystallizable (Fc) of immunoglobulin-gamma isotypes (IgGs), a prerequisite for antibody glycan sialylation and related anti-inflammatory effector functions (PubMed : 27872474, PubMed : 29133956, PubMed : 36280670, PubMed : 37632720). Can also transfer a Gal residue to free GlcNAc to form N-acetyllactosamine (PubMed : 16157350). With LALBA/alpha-lactalbumin forms the lactose synthase complex responsible for production of large amounts of lactose in the lactating mammary gland. Interaction with LALBA alters the sugar substrate specificity of the catalytic domain, enabling high affinity binding of glucose and its transformation to lactose (PubMed : 16157350).. Isoform Long. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, like those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. Acts as a sperm receptor for ZP3 O-linked glycans on the zona pellucida leading to activation of G-protein signaling and acrosome reaction.. Processed beta-1,4-galactosyltransferase 1. The secreted form is proficient in galactosyltransferase activity and could be involved in glycan remodeling in biological fluids.
See full target information B4GALT1
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