Human B7-H7/HHLA2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human B7-H7/HHLA2 in Cell Lysate samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.156 - 10 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.05 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Alternative names
HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, HHLA2
What's included?
Human B7-H7/HHLA2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human B7-H7/HHLA2 in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.156 - 10 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.05 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. = 2.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. = 2.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 86 | Range 83 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human B7-H7/HHLA2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of B7-H7/HHLA2 protein in Cell Lysate. Quantitate Human B7-H7/HHLA2 with 0.05 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 17pack (10 x 96well plates).
Also available in a fully validated 384well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human B7-H7/HHLA2 ELISA Kit (ab323928)
Interpolated concentration of native B7-H7/HHLA2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL A-204 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human B7-H7/HHLA2 ELISA Kit (ab323928)
Example of human B7-H7/HHLA2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human B7-H7/HHLA2 ELISA Kit (ab323928)
Interpolated concentration of native B7-H7/HHLA2 was measured in duplicate at different sample concentrations. Undiluted samples are 250 µg/mL A-204 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human B7-H7/HHLA2 ELISA Kit (ab323928)
Example of human B7-H7/HHLA2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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