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AB267637

Human BACE-1 ELISA Kit

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(1 Publication)

Human BACE-1 ELISA Kit is a Sandwich ELISA for the measurement of Human BACE-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

BACE, KIAA1149, BACE1, Beta-secretase 1, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, ASP2, Asp 2, Beta-site APP cleaving enzyme 1

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Sandwich ELISA - Human BACE-1 ELISA Kit (AB267637)
  • sELISA

Supplier Data

Sandwich ELISA - Human BACE-1 ELISA Kit (AB267637)

Human BACE-1 ELISA kit (ab267637) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 ng/mL

Range

1.024 - 250 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human BACE-1 ELISA Kit is designed for the quantitative determination of BACE-1 in cell culture supernatants, plasma and serum samples.

ΔNote: Human BACE-1 concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for human BACE-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BACE-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BACE-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BACE-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA antibody pair detects human BACE-1, it also can recognize mouse BACE-1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "69 - 121 %", "average": "= 99" }, { "sample": "Plasma", "range": "78 - 100 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "78 - 108 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267637-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267637-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267637-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267637-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267637-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human BACE-1 coated Microplate (12 x 8 wells)", "number":"AB267637-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human BACE-1 Detection Antibody", "number":"AB267637-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267637-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human BACE-1 Standard (Lyophilized)", "number":"AB267637-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BACE1 also known as beta-site APP cleaving enzyme 1 or beta-secretase plays an important role in the cleavage of amyloid precursor protein (APP). This cleavage results in the production of amyloid-beta peptides which are associated with Alzheimer's disease. BACE1 is a membrane-bound aspartic protease and has a molecular weight of approximately 50100 Da. The enzyme expresses mostly in the brain's neurons and some secretory tissues.
Biological function summary

BACE1 initiates the amyloidogenic pathway of APP processing which involves amyloid-beta generation. BACE1 doesn't function alone but acts as part of a complex that aids in protein substrate recognition and processing. Its activity contributes to physiological processes like myelination and axonal guidance indicating its importance beyond amyloid production.

Pathways

BACE1 is integral to the amyloidogenic pathway which is important in Alzheimer's disease development. It interacts with proteins such as presenilin 1 a part of the gamma-secretase complex that further processes the amyloid-beta precursor. Furthermore BACE1 links to synaptic functions and neural signaling pathways highlighting its multifaceted roles.

BACE1 holds significance in Alzheimer's disease due to its role in amyloid-beta peptide production. This connection has led to the development of BACE1 inhibitors as potential therapeutic agents. Additionally BACE1’s involvement in other neural functions ties it to cognitive impairments. It also relates to APP through its function in Alzheimer's suggesting targeted strategies for treatment could involve modulating BACE1 activity.
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Product protocols

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Target data

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed : 10656250, PubMed : 10677483, PubMed : 20354142). Cleaves CHL1 (By similarity).
See full target information BACE1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Drug design, development and therapy 15:2013-2024 PubMed34012254

2021

Epigallocatechin-3-Gallate Provides Protection Against Alzheimer's Disease-Induced Learning and Memory Impairments in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shanji Nan,Peng Wang,Yizhi Zhang,Jia Fan
View all publications
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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