Human BACE1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BACE1 with a sensitivity of 28.614 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 28.614 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity).
Alternative names
BACE, KIAA1149, BACE1, Beta-secretase 1, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, ASP2, Asp 2, Beta-site APP cleaving enzyme 1
What's included?
Human BACE1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BACE1 with a sensitivity of 28.614 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 28.614 pg/mL
- General recovery
- 88%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 5.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 1.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 88 | Range 83 - 91 % |
Sample type EDTA Plasma | Average % = 87 | Range 82 - 92 % |
Sample type Heparin Plasma | Average % = 92 | Range 86 - 100 % |
Sample type Cell culture media | Average % = 96 | Range 93 - 97 % |
Sample type Cerebral Spinal Fluid | Average % = 89 | Range 80 - 100 % |
Sample type Tissue Extracts | Average % = 92 | Range 89 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human BACE1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BACE1 protein in Serum, EDTA Plasma, Hep Plasma, Cell culture media, Cerebral Spinal Fluid, and Tissue extracts. Quantitate Human BACE1 with 28.61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
8 product images
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Interpolated concentration of native BACE1 was measured in duplicate at different sample concentrations. Undiluted samples are 50% cerebrospinal fluid. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Interpolated concentration of native BACE1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 125 µg/mL human cortex tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Example of human BACE1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Example of human BACE1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Serum of seven individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean BACE1 concentration was determined to be 1.26 ng/mL with a range of 0.62 – 2.11 ng/mL.
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Example of human BACE1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Interpolated concentration of native BACE1 was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL human cortex tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human BACE1 ELISA Kit (ab322437)
Example of human BACE1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
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