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AB119579

Human BAFF ELISA Kit (TNFSF13B)

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(4 Publications)

Human BAFF ELISA Kit (TNFSF13B) is a Sandwich ELISA for the measurement of Human BAFF (TNFSF13B) in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

CD257, BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, TNFSF13B, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, BLyS, TALL-1

1 Images
Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB119579)
  • sELISA

Supplier Data

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB119579)

Representative Standard Curve using ab119579.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

62.5 - 4000 pg/mL

Assay Platform

Microplate

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Complementary Products

Assay Kits

AB119505

Human APRIL ELISA Kit

Sandwich ELISA - Human APRIL ELISA Kit (AB119505)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Assay Kits

AB213841

Human TNFSF11 ELISA Kit (RANKL)

Sandwich ELISA - Human TNFSF11 ELISA Kit (RANKL) (AB213841)

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Primary Antibodies

AB224710

Anti-BAFF antibody [EPR22238]

RabMAb|Recombinant

Flow Cytometry - Anti-BAFF antibody [EPR22238] (AB224710)

  • 1
  • 1
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Reactivity data

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Product details

Abcam's Human BAFF (TNFSF13B) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BAFF (TNFSF13B) in cell culture supernatants, serum and plasma (heparin, EDTA).

A BAFF (TNFSF13B) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BAFF (TNFSF13B) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BAFF (TNFSF13B) amount of sample captured in plate.

Get higher sensitivity in only 90 minutes with Human BAFF ELISA Kit (TNFSF13B) (ab188391) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1229 pg/mL", "standardDeviation": "79.88", "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "127 pg/mL", "standardDeviation": "5.46", "coefficientOfVariability": "= 4.3" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "522 pg/mL", "standardDeviation": "38.62", "coefficientOfVariability": "= 7.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1268 pg/mL", "standardDeviation": "103.97", "coefficientOfVariability": "= 8.2" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "124 pg/mL", "standardDeviation": "5.7", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "548 pg/mL", "standardDeviation": "47.67", "coefficientOfVariability": "= 8.7" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.
Biological function summary

BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.

Pathways

BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.

BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.
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Product protocols

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Target data

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.. Isoform 3. Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
See full target information TNFSF13B
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112767 PubMed37440412

2023

Macrophages drive KSHV B cell latency.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Szymula,Gabriela Samayoa-Reyes,Sidney Ogolla,Bing Liu,Shijun Li,Athira George,Nicholas Van Sciver,Rosemary Rochford,J Pedro Simas,Kenneth M Kaye

Brain communications 5:fcad060 PubMed36993946

2023

B lymphocyte responses in Parkinson's disease and their possible significance in disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten M Scott,Yen Ting Chong,Seoyoung Park,Ruwani S Wijeyekoon,Shaista Hayat,Rebeccah J Mathews,Zachary Fitzpatrick,Pam Tyers,Georgia Wright,Jennifer Whitby,Roger A Barker,Michele T Hu,Caroline H Williams-Gray,Menna R Clatworthy

Parasite immunology 40:e12580 PubMed30102786

2018

Vivax infection alters peripheral B-cell profile and induces persistent serum IgM.

Applications

Unspecified application

Species

Unspecified reactive species

Mandar Patgaonkar,Fabien Herbert,Krushali Powale,Prajakta Gandhe,Nithya Gogtay,Urmila Thatte,Sylviane Pied,Shobhona Sharma,Sulabha Pathak

Scientific reports 6:29268 PubMed27383531

2016

Serum BAFF levels, Methypredsinolone therapy, Epstein-Barr Virus and Mycobacterium avium subsp. paratuberculosis infection in Multiple Sclerosis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Mameli,Eleonora Cocco,Jessica Frau,Giannina Arru,Elisa Caggiu,Maria Giovanna Marrosu,Leonardo A Sechi
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Product promise

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