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AB188391

Human BAFF ELISA Kit (TNFSF13B)

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(3 Publications)

Human BAFF ELISA Kit (TNFSF13B) is a single-wash 90-min Simplestep used to quantify Human BAFF (TNFSF13B) with a sensitivity of 12.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

CD257, BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, TNFSF13B, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, BLyS, TALL-1

3 Images
Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)
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Supplier Data

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)

Titration of individual donor serum samples to within working range of the assay.

Quantification of BAFF interpolated from standard curve and multiplied by dilution factor.

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)
  • sELISA

Supplier Data

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)

Example of BAFF standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)
  • sELISA

Unknown

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)

Titration of Human Plasma Samples within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 12.7 pg/mL

Range

0.08 - 5 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human BAFF ELISA Kit (TNFSF13B) (ab188391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BAFF (TNFSF13B) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BAFF (TNFSF13B) with 12.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

B-cell activating factor (BAFF) protein that is encoded by the TNFSF13B gene. BAFF protein is expressed in B-cell lineage cells which functions as a cytokine and is also a member of the tumor necrosis factor (TNF) family of ligands. The BAFF cytokine acts as a potent B-cell activator promoting the proliferation and differentiation of B-cells. This protein is expressed as a membrane-bound type II transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The membrane bound form can be cleaved from the membrane, generating a soluble protein fragment. BAFF steady-state concentrations depend on B‑cells and also on the expression of BAFF-binding receptors.

Inadequate levels of BAFF will fail to activate B-cells to produce enough immunoglobulin and will lead to immunodeficiency. Excessive level of BAFF causes abnormally high antibody production, results in systemic lupus erythmatosis, rheumatoid arthritis, and many other autoimmune diseases.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 115 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "98 - 115 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "101 - 108 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "100 - 111 %", "average": "= 106" }, { "sample": "Cell culture media", "range": "106 - 110 %", "average": "= 108" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.
Biological function summary

BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.

Pathways

BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.

BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.
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Product protocols

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Target data

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.. Isoform 3. Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
See full target information TNFSF13B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 11: PubMed35012014

2022

Early Reconstitution of Antibody Secreting Cells after Allogeneic Stem Cell Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Hinterleitner,Clemens Hinterleitner,Elke Malenke,Birgit Federmann,Ursula Holzer,Martin Müller,Wolfgang A Bethge,Stefan Wirths

Molecular medicine reports 21:795-805 PubMed31974601

2020

BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF‑κB signaling pathway in glomerular mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Cao,Guoyuan Lu,Xiaolan Chen,Xu Chen,Naifeng Guo,Wenwen Li

The Journal of allergy and clinical immunology 135:1380-4.e1-5 PubMed25512081

2014

RAC2 loss-of-function mutation in 2 siblings with characteristics of common variable immunodeficiency.

Applications

ELISA

Species

Human

Omar K Alkhairy,Nima Rezaei,Robert R Graham,Hassan Abolhassani,Stephan Borte,Kjell Hultenby,Chenglin Wu,Asghar Aghamohammadi,David A Williams,Timothy W Behrens,Lennart Hammarström,Qiang Pan-Hammarström
View all publications
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