JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213839

Human BAFF-R ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Human BAFF-R ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human BAFF-R in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

CD268, BAFFR, BR3, TNFRSF13C, Tumor necrosis factor receptor superfamily member 13C, B-cell-activating factor receptor, BAFF receptor, BLyS receptor 3, BAFF-R

1 Images
Sandwich ELISA - Human BAFF-R ELISA Kit (AB213839)
  • sELISA

Supplier Data

Sandwich ELISA - Human BAFF-R ELISA Kit (AB213839)

Human TNFRSF13C ELISA Kit (BAFFR) (ab213839) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

78 - 5000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A goat polyclonal antibody specific for BAFF-R has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.

BAFF-R (also known as Tumor necrosis factor receptor superfamily member 13C or B-cell-activating factor receptor) is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "2531 pg/mL", "standardDeviation": "227.05", "coefficientOfVariability": "= 8.9" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "2750 pg/mL", "standardDeviation": "206.25", "coefficientOfVariability": "= 7.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "683 pg/mL", "standardDeviation": "49.85", "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "700 pg/mL", "standardDeviation": "62.3", "coefficientOfVariability": "= 8.9" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "147 pg/mL", "standardDeviation": "10.29", "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "156 pg/mL", "standardDeviation": "13.88", "coefficientOfVariability": "= 8.9" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213839-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213839-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human BAFF-R antibody", "number":"AB213839-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human BAFF-R coated Microplate (12 x 8 wells)", "number":"AB213839-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213839-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213839-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213839-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213839-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213839-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Recombinant Human BAFF-R Standard", "number":"AB213839-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213839-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213839-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF-R also known as B-cell activating factor receptor or BAFF receptor plays a critical role in B-cell development and survival. BAFF-R is a receptor for the BAFF protein also referred to as B lymphocyte stimulator (BLyS). BAFF-R has a molecular mass of approximately 19 kDa. The expression of BAFF-R is found on B cells with higher presence on mature B cells compared to immature ones. The receptor is recognized as the main signaling partner for BAFF which forms the basis for many of its immune functions.
Biological function summary

BAFF-R is essential for the maturation and maintenance of peripheral B cells. The receptor contributes to the regulation of immune responses by ensuring B-cell homeostasis. It does not generally form part of a larger protein complex but instead functions as a membrane-bound receptor that transmits signals upon binding with the BAFF ligand. This signaling cascade supports the survival and proliferation of B cells which is fundamental for the adaptive immune system.

Pathways

BAFF-R is deeply involved in the NF-κB signaling pathway which is important for immune regulation and cell survival. Upon binding with BAFF BAFF-R recruits associated proteins to initiate the pathway and as a result promotes gene transcription important for B-cell function. Another connected pathway involves AKT a protein kinase that regulates B-cell metabolism and differentiation. Both NF-κB and AKT pathways help facilitate the immune functions tied to B cells directly through BAFF-R signaling.

BAFF-R demonstrates a notable link with autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. Overexpression of BAFF impacting interaction with BAFF-R contributes to such conditions by promoting the survival of autoreactive B cells. In SLE BAFF works with another receptor TACI influencing the proliferation of autoreactive cells. The persistent activation and dysfunction of the immune response through BAFF-R interactions exacerbate these autoimmune conditions.
style
left-column

Product protocols

style
left-column

Target data

B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
See full target information TNFRSF13C
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Microbiology and immunology 67:377-387 PubMed37340676

2023

Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sjögren's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangxi Xu,Zhengdong Shen,Yun Du,Shai Zhang,Yajing Xi,Yuting Zhang,Dan Wang,Yuelan Zhu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB188391

Human BAFF ELISA Kit (TNFSF13B)

SimpleStep

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)

  • 0
  • 0
Primary Antibodies

AB224710

Anti-BAFF antibody [EPR22238]

RabMAb|Recombinant

Flow Cytometry - Anti-BAFF antibody [EPR22238] (AB224710)

  • 1
  • 1
Primary Antibodies

AB188868

Anti-BAFF-R antibody [EPR14633]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody [EPR14633] (AB188868)

  • 0
  • 0
Primary Antibodies

AB229902

Anti-DLL3 antibody [EPR22592-18]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] (AB229902)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com