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Human BATF3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human BATF3 in Human in Cell culture supernatant, Plasma, Serum samples.

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Sandwich ELISA - Human BATF3 ELISA Kit (AB313511), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
50 - 10000 pg/mL
Sensitivity
= 50 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

AP-1 family transcription factor that controls the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Required for development of CD8-alpha(+) classical dendritic cells (cDCs) and related CD103(+) dendritic cells that cross-present antigens to CD8 T-cells and produce interleukin-12 (IL12) in response to pathogens (By similarity). Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
900X HRP-Streptavidin
1 x 200 µL
Biotinylated Human BATF3 Detection Antibody
2 x 1 Vial
Human BATF3 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human BATF3 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human BATF3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human BATF3 in Human in Cell culture supernatant, Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
50 - 10000 pg/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 50 pg/mL

Recovery

Sample specific recovery

Sample type
Cell culture media
Average %
= 89.47
Range
73 - 132 %
Sample type
Plasma
Average %
= 88.05
Range
71 - 141 %
Sample type
Serum
Average %
= 88.17
Range
79 - 131 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human BATF3 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BATF3 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human BATF3 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTSL-1/Punctin-1, AMSH/STAMBP, Annexin V, Bora, Cadherin-17, Caveolin-2, CD2, CD200 R1, CHST3, COMT, Cystatin SA, Dopamine beta-Hydroxylase, Desmin, Exostosinlike 3, Ficolin-1, FosB/GOS3, FRS2, GATA-5, GFAP, GLI-3, HepaCAM, ARNT/HIF-1 beta, 17 betaHSD1/HSD17B1, IDO, Kallikrein 1, Kell, MDL-1/CLEC5A, NPDC-1, Numb, Olig2, p63/TP73L, Pax3, Semaphorin 4D, Sphingosine Kinase 1/SPHK1, TAZ/WWTR1, TC-PTP, Transglutaminase 3/TGM3, TPST2, TREML1/TLT-1.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human BATF3 ELISA Kit (ab313511), expandable thumbnail

    Sandwich ELISA - Human BATF3 ELISA Kit (ab313511)

    Example of standard curve using ab313511. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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