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AB99981

Human BCA1 ELISA Kit

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Human BCA1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human BCA1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

BCA1, BLC, SCYB13, CXCL13, C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1

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Sandwich ELISA - Human BCA1 ELISA Kit (AB99981)
  • sELISA

Supplier Data

Sandwich ELISA - Human BCA1 ELISA Kit (AB99981)

Representative Standard Curve using ab99981.

Sandwich ELISA - Human BCA1 ELISA Kit (AB99981)
  • sELISA

Supplier Data

Sandwich ELISA - Human BCA1 ELISA Kit (AB99981)

Representative Standard Curve using ab99981.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 1.5 pg/mL

Range

1.37 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's BCA1 (BLC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BCA1 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human BCA1 ELISA Kit (CXCL13) (ab179881) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 105 %", "average": "= 95.62" }, { "sample": "Serum", "range": "82 - 105 %", "average": "= 95.79" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 93.68" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99981-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99981-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99981-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human BCA1", "number":"AB99981-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human BCA1 Standard (lyophilized)", "number":"AB99981-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99981-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99981-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"BCA1 Microplate (12 x 8 wells)", "number":"AB99981-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB99981-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99981-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
See full target information CXCL13
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com