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AB304935

Human BCAM (CD239) ELISA Kit

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Human BCAM (CD239) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BCAM (CD239) with a sensitivity of 5.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD239, LU, MSK19, BCAM, Basal cell adhesion molecule, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein

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Sandwich ELISA - Human BCAM (CD239) ELISA Kit (AB304935)
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Supplier Data

Sandwich ELISA - Human BCAM (CD239) ELISA Kit (AB304935)

Example of human BCAM standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human BCAM (CD239) ELISA Kit (AB304935)
  • sELISA

Supplier Data

Sandwich ELISA - Human BCAM (CD239) ELISA Kit (AB304935)

Interpolated concentration of native BCAM was measured in duplicate at different sample concentrations. Undiluted samples are 50 µg/mL HeLa Cell Extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.9 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human BCAM (CD239) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BCAM (CD239) protein in cell extractst. Quantitate Human BCAM (CD239) with 5.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.1" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "87 - 137 %", "average": "= 113" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab304935-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB304935-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB304935-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB304935-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB304935-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB304935-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human BCAM (CD239) Lyophilized Recombinant Protein", "number":"AB304935-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BCAM (CD239) Capture Antibody", "number":"AB304935-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB304935-CMP05", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB304935-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB304935-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BCAM (CD239) Detector Antibody", "number":"AB304935-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD239 also known as basal cell adhesion molecule (BCAM) is an integral protein with a mass of about 60 kDa. This protein is primarily expressed in endothelial cells red blood cells and various epithelial tissues. BCAM plays a significant role in cell adhesion acting as a receptor for the extracellular matrix component laminin specifically laminin-511/521. It helps cells attach an essential role for maintaining tissue integrity and cellular communication.
Biological function summary

BCAM influences processes related to cell adhesion and motility. Although not explicitly part of a large complex it interacts closely with the cytoskeleton and mediators of signal transduction. Functions of BCAM involve linking extracellular matrix signals to the intracellular environment impacting cell movement and stabilization within tissues. It also modulates cellular responses to external mechanical stresses playing essential roles in maintaining normal cell function.

Pathways

BCAM is involved in several critical signaling processes including integrin-mediated adhesion and the MAPK/ERK pathway. How BCAM affects these pathways is through its interaction with integrins and elements related to cellular migration and proliferation. Within these pathways related proteins like laminins and other integrin subunits contribute to its adhesion properties and signaling functions enabling coordinated cellular responses to environmental cues.

BCAM is notably associated with sickle cell disease and certain cancers. In sickle cell disease the altered expression or function of BCAM on red blood cells causes abnormal adhesion to the endothelium leading to vaso-occlusion. This adhesion is often exacerbated by interactions with other proteins like L-selectin. In cancer BCAM overexpression correlates with increased tumor cell migration and invasion influencing metastasis and patient prognosis. These associations highlight BCAM's critical role in disease pathology and potential as a therapeutic target.
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Product protocols

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Target data

Transmembrane glycoprotein that functions as both a receptor and an adhesion molecule playing a crucial role in cell adhesion, motility, migration and invasion (PubMed : 9616226, PubMed : 31413112). Extracellular domain enables binding to extracellular matrix proteins, such as laminin, integrin and other ligands while its intracellular domain interacts with cytoskeletal proteins like hemoglobin, facilitating cell signal transduction (PubMed : 17158232). Serves as a receptor for laminin alpha-5/LAMA5 to promote cell adhesion (PubMed : 15975931). Mechanistically, JAK2 induces BCAM phosphorylation and activates its adhesion to laminin by stimulating a Rap1/AKT signaling pathway in the absence of EPOR (PubMed : 23160466).
See full target information BCAM
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB134110

Anti-CD239/BCAM antibody [EPR4165]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

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Proteins & Peptides

AB276950

Recombinant Mouse BCAM protein (His tag)

SDS-PAGE - Recombinant Mouse BCAM protein (His tag) (AB276950)

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Cell Lines & Lysates

AB269619

Human BCAM knockout A-431 cell line

Next Generation Sequencing - Human BCAM knockout A-431 cell line (AB269619)

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