Human Bcl-2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Bcl-2 in Human in Plasma, Cell culture extracts, Serum samples.
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- Microorganisms 12:2024Induces Apoptosis in Melanoma and Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOana Budu,Alexandra Mioc,Codruta Soica,Florina Caruntu,Andreea Milan,Camelia Oprean,Daniel Lighezan,Slavita Rotunjanu,Viviana Ivan,Christian BanciuPubMed 38258008
- Nanomaterials (Basel, Switzerland) 13:2023An Analysis of the Toxicity, Antioxidant, and Anti-Cancer Activity of Cinnamon Silver Nanoparticles in Comparison with Extracts and Fractions of Cinnamomum Cassia at Normal and Cancer Cell Levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY G El-Baz,A Moustafa,M A Ali,G E El-Desoky,S M Wabaidur,M M FaisalPubMed 36903823
- Molecules (Basel, Switzerland) 28:2023The Antimelanoma Biological Assessment of Triterpenic Acid Functionalized Gold Nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarius Mioc,Alexandra Mioc,Roxana Racoviceanu,Roxana Ghiulai,Alexandra Prodea,Andreea Milan,Lucian Barbu Tudoran,Camelia Oprean,Viviana Ivan,Codruța ȘoicaPubMed 36615613
- Probiotics and antimicrobial proteins 15:1271-12862022Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNessrin Saleh,Hoda E Mahmoud,Hoda Eltaher,Maged Helmy,Labiba El-Khordagui,Ahmed A HusseinPubMed 36030493
- Molecules (Basel, Switzerland) 26:2021Nano Milk Protein-Mucilage Complexes: Characterization and Anticancer Effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Ali Abd El-Maksoud,Amal I A Makhlouf,Ammar B Altemimi,Ismail H Abd El-Ghany,Amr Nassrallah,Francesco Cacciola,Tarek Gamal AbedelmaksoudPubMed 34770781
- International journal of molecular sciences 22:2021Influence of Ce Substitution on Antimicrobial and Antibiofilm Properties of ZnCeFeO Nanoparticles (X = 0.0, 0.02, 0.04, 0.06, and 0.08) Conjugated with Ebselen and Its Role Subsidised with γ-Radiation in Mitigating Human TNBC and Colorectal Adenocarcinoma Proliferation In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed K Abdel-Rafei,Noura M Thabet,M I A Abdel Maksoud,M Abd Elkodous,Go Kawamura,Atsunori Matsuda,A H Ashour,Ahmed I El-Batal,Gharieb S El-SayyadPubMed 34576334
- Saudi journal of biological sciences 28:4137-41462021E74-like factor 3 suppresses microRNA-485-5p transcription to trigger growth and metastasis of ovarian cancer cells with the involvement of CLDN4/Wnt/β-catenin axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Kuang,Li'an LiPubMed 34354393
- Immunopharmacology and immunotoxicology 43:370-3792021MiR-485-3p promotes proliferation of osteoarthritis chondrocytes and inhibits apoptosis via Notch2 and the NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunping Zhou,Zandong Zhao,Liang Yan,Jie YangPubMed 33961511
- Clinical, cosmetic and investigational dermatology 13:957-9712020Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-β Signaling Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoxiu Cao et. al.PubMed 33364805
- Analytical cellular pathology (Amsterdam) 2020:37319532020Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeilei Xu et. al.PubMed 33123457
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.5 - 32 ng/mL
- Sensitivity
- = 0.5 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
What's included?
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Human Bcl-2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Bcl-2 in Human in Plasma, Cell culture extracts, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.5 - 32 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.5 ng/mL
- General recovery
- 71%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 8.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 12 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Bcl-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab119506) is designed for accurate quantitative measurement of Human Bcl-2 concentrations in cell lysates, serum and plasma.
Bcl-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Bcl-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bcl-2 captured on the plate.
Get results in 90 minutes with Human Bcl-2 ELISA Kit (ab202411) from our SimpleStep ELISA® range.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
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Sandwich ELISA - Human Bcl-2 ELISA Kit (ab119506)
Representative Standard Curve using ab119506.
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