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AB119506

Human Bcl-2 ELISA Kit

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(30 Publications)

Human Bcl-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Bcl-2 in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Apoptosis regulator Bcl-2, BCL2

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Sandwich ELISA - Human Bcl-2 ELISA Kit (AB119506)
  • sELISA

Supplier Data

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB119506)

Representative Standard Curve using ab119506.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.5 ng/mL

Range

0.5 - 32 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Bcl-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab119506) is designed for accurate quantitative measurement of Human Bcl-2 concentrations in cell lysates, serum and plasma.

Bcl-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Bcl-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bcl-2 captured on the plate.

Get results in 90 minutes with Human Bcl-2 ELISA Kit (ab202411) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
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Product protocols

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Target data

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).
See full target information BCL2
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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Chemistry & biodiversity 22:e202401673 PubMed39353043

2024

Discovery of New Pyrazole-Tosylamide Derivatives as Apoptosis Inducers Through BCL-2 Inhibition and Caspase-3 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Burak Kuzu,Ege Arzuk

Heliyon 10:e26850 PubMed38495151

2024

The p53-mediated cell cycle regulation is a potential mechanism for emodin-suppressing osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Shuli Hao,Guangyou Wei,Xiangyu Liu,Yang Miao

Microorganisms 12: PubMed38258008

2024

Induces Apoptosis in Melanoma and Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Oana Budu,Alexandra Mioc,Codruta Soica,Florina Caruntu,Andreea Milan,Camelia Oprean,Daniel Lighezan,Slavita Rotunjanu,Viviana Ivan,Christian Banciu

Cancer biology & therapy 24:2246198 PubMed37773732

2023

inhibits the growth and metastasis of liver cancer by downregulating miR-9-5p and upregulating PDK4.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Si,Liyin Huang,Ting Liang,Ping Huang,Hongyu Zhang,Mingmin Zhang,Xiaoling Zhou

Nanomaterials (Basel, Switzerland) 13: PubMed36903823

2023

An Analysis of the Toxicity, Antioxidant, and Anti-Cancer Activity of Cinnamon Silver Nanoparticles in Comparison with Extracts and Fractions of Cinnamomum Cassia at Normal and Cancer Cell Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Y G El-Baz,A Moustafa,M A Ali,G E El-Desoky,S M Wabaidur,M M Faisal

Molecules (Basel, Switzerland) 28: PubMed36615613

2023

The Antimelanoma Biological Assessment of Triterpenic Acid Functionalized Gold Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Mioc,Alexandra Mioc,Roxana Racoviceanu,Roxana Ghiulai,Alexandra Prodea,Andreea Milan,Lucian Barbu Tudoran,Camelia Oprean,Viviana Ivan,Codruța Șoica

Journal of enzyme inhibition and medicinal chemistry 38:203-215 PubMed36382444

2022

Design, synthesis and antitumor evaluation of novel pyrazolo[3,4-]pyrimidines incorporating different amino acid conjugates as potential DHFR inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Ibrahim M Salem,Samia M Mostafa,Ismail Salama,Osama I El-Sabbagh,Wael A H Hegazy,Tarek S Ibrahim

Probiotics and antimicrobial proteins 15:1271-1286 PubMed36030493

2022

Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nessrin Saleh,Hoda E Mahmoud,Hoda Eltaher,Maged Helmy,Labiba El-Khordagui,Ahmed A Hussein

Molecules (Basel, Switzerland) 26: PubMed34770781

2021

Nano Milk Protein-Mucilage Complexes: Characterization and Anticancer Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Ali Abd El-Maksoud,Amal I A Makhlouf,Ammar B Altemimi,Ismail H Abd El-Ghany,Amr Nassrallah,Francesco Cacciola,Tarek Gamal Abedelmaksoud

International journal of molecular sciences 22: PubMed34576334

2021

Influence of Ce Substitution on Antimicrobial and Antibiofilm Properties of ZnCeFeO Nanoparticles (X = 0.0, 0.02, 0.04, 0.06, and 0.08) Conjugated with Ebselen and Its Role Subsidised with γ-Radiation in Mitigating Human TNBC and Colorectal Adenocarcinoma Proliferation In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed K Abdel-Rafei,Noura M Thabet,M I A Abdel Maksoud,M Abd Elkodous,Go Kawamura,Atsunori Matsuda,A H Ashour,Ahmed I El-Batal,Gharieb S El-Sayyad
View all publications
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