AB272102

Human Bcl-2 ELISA Kit

SimpleStep
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(4 Publications)

Human Bcl-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Bcl-2 with a sensitivity of 38.08 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Apoptosis regulator Bcl-2, BCL2

3 Images

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB272102)

sELISA

Supplier Data

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB272102)

Interpolated concentrations of native Bcl-2 in human MCF7, U266B1, and thymus homogenate extract samples based on 250, 62.50, and 250 μg/mL extract loads respectively.

The concentrations of Bcl-2 were measured in duplicate and interpolated from the Bcl-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bcl-2 concentration was determined to be 4,668 pg/mL in MCF7, 7,515 pg/ml in U266B1, and 1,815 pg/ml in thymus homogenate extract.

sELISA

Supplier Data

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB272102)

Human Bcl-2 standard curve comparison.

Standard Curve comparison between the original Human Bcl-2 SimpleStep ELISA kit (ab202411) and current Human MMP3 SimpleStep ELISA kit (ab272102).

sELISA

Supplier Data

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB272102)

Example of human Bcl-2 standard curve in 1X Cell Extraction Buffer PTR.

The Bcl-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 38.08 pg/mL

Range

195.31 - 12500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Bcl-2 ELISA Kit (ab272102) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Bcl-2 protein in human cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Bcl-2 with 38.08 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Bcl-2 is the founding member of the apoptotic-regulating Bcl-2 family of proteins. It is considered an apoptosis suppressor in many cell systems and has been shown to interact with many other proteins involved in cell cycle regulation, autophagy, mitochondrial membrane stability, and oxidative stress response, among many others. As such an important protein involved in cell regulation, mutation or damage to the protein's structure or function are critically linked to a variety of cancers. In particular, a chromosomal translocation occurs in follicular lymphoma which causes the Bcl-2 gene to relocate next to the heavy chain immunoglobulin locus. The unregulated expression of the fusion protein causes an excessively high amount of Bcl-2, disrupting the cell's apoptosis regulation and leading to unregulated cell growth.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "103 - 111 %", "average": "= 107" }, { "sample": "Tissue Extracts", "range": "111 - 116 %", "average": "= 113" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272102-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272102-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272102-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272102-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272102-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272102-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB272102-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Bcl-2 Capture Antibody", "number":"AB272102-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Bcl-2 Lyophilized Recombinant Protein", "number":"AB272102-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Bcl-2 Detector Antibody", "number":"AB272102-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272102-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272102-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.

Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.

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Product protocols

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Target data

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).

See full target information BCL2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 19:7323-7352 PubMed39055376

2024

FeO-Coated CNTs-Gum Arabic Nano-Hybrid Composites Exhibit Enhanced Anti-Leukemia Potency Against AML Cells via ROS-Mediated Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Alyaa S Abdel Halim,Mohamed A M Ali,Fawad Inam,Abdulrahman M Alhalwan,Walid M Daoush

Foods (Basel, Switzerland) 13: PubMed38672825

2024

Bioactive Potential of Olive Mill Waste Obtained from Cultivars Grown in the Island of Malta.

Applications

Unspecified application

Species

Unspecified reactive species

Frederick Lia,Karen Attard

Biomolecules 14: PubMed38397453

2024

Synthesis, Characterization, Antioxidant, and Anticancer Activity against Colon Cancer Cells of Some Cinnamaldehyde-Based Chalcone Derivatives.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A El-Atawy,Demiana H Hanna,Ali H Bashal,Hoda A Ahmed,Eida M Alshammari,Ezzat A Hamed,Abdullah R Aljohani,Alaa Z Omar

PloS one 16:e0258312 PubMed34793477

2021

Role of long intergenic non-protein coding RNA 01857 in hepatocellular carcinoma malignancy via the regulation of the microRNA-197-3p/anterior GRadient 2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangang Bi,Yusheng Guo,Qi Li,Liping Liu,Shiyun Bao,Ping Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Bcl-2 ELISA Kit

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB272102)

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB272102)

Sandwich ELISA - Human Bcl-2 ELISA Kit (AB272102)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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