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AB263875

Human BCMA ELISA Kit

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(2 Publications)

Human BCMA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BCMA with a sensitivity of 10.81 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD269, BCM, BCMA, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein

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ELISA - Human BCMA ELISA Kit (AB263875)
  • ELISA

Supplier Data

ELISA - Human BCMA ELISA Kit (AB263875)
Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
  • sELISA

Supplier Data

Sandwich ELISA - Human BCMA ELISA Kit (AB263875)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 13.58 ng/mL with a range of 9.70 – 18.54 ng/mL.

Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
  • sELISA

Supplier Data

Sandwich ELISA - Human BCMA ELISA Kit (AB263875)

Interpolated concentrations of native BCMA in human cell culture supernatant and pooled-sex urine samples.

The concentrations of BCMA were measured in duplicates, interpolated from the BCMA standard curves and corrected for sample dilution. Undiluted samples are as follows : U266B1 supernatant 1 : 200 and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 142.48 ng/mL in U266B1 supernatant and 3.41 ng/mL in pooled-sex urine.

Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
  • sELISA

Supplier Data

Sandwich ELISA - Human BCMA ELISA Kit (AB263875)

Interpolated concentrations of native BCMA in human serum and plasma samples.

The concentrations of BCMA were measured in duplicates, interpolated from the BCMA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 25.96 ng/mL in serum, 17.77 ng/mL in plasma (citrate), 18.02 ng/ml in plasma (heparin), and 17.02 ng/mL in plasma (EDTA).

Sandwich ELISA - Human BCMA ELISA Kit (AB263875)
  • sELISA

Supplier Data

Sandwich ELISA - Human BCMA ELISA Kit (AB263875)

Example of human BCMA standard curve in Sample Diluent NS.

The BCMA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.81 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human BCMA ELISA Kit ab263875 is a rapid single-wash 90-min Sandwich ELISA to measure Human BCMA in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 10.81 pg/mL.

How the assay works

Human BCMA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human BCMA ELISA Kit ab263875 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-BCMA antibody [EPR22457-30] - BSA and Azide free (Capture) ab259767
- Anti-BCMA antibody [EPR22457-28] - BSA and Azide free (Detector) ab267460

Tumor necrosis factor receptor superfamily member 17 (BCMA) is a receptor involved in B cell survival. BCMA activates the NF-kappa-B and JNK pathways. Aberrant expression of BCMA is observed on malignant B cells, including during multiple myeloma, Hodgkin's lymphoma, and Waldenstrom's macroglobulinemia.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "107 - 112 %", "average": "= 109" }, { "sample": "Serum", "range": "99 - 110 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "102 - 120 %", "average": "= 111" }, { "sample": "Urine", "range": "101 - 116 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "87 - 104 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "105 - 112 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "101 - 108 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab263875-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263875-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB263875-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263875-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB263875-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB263875-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB263875-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human BCMA Lyophilized Recombinant Protein", "number":"AB263875-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BCMA Capture Antibody", "number":"AB263875-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB263875-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BCMA Detector Antibody", "number":"AB263875-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
See full target information TNFRSF17
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 109:3670-3680 PubMed38572568

2024

Soluble B-cell maturation antigen in lacrimal fluid as a potential biomarker and mediator of keratopathy in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Umair Munawar,Johanna Theuersbacher,Maximilian J Steinhardt,Xiang Zhou,Seungbin Han,Silvia Nerreter,Cornelia Vogt,Shilpa Kurian,Thorsten Keller,Ann-Katrin Regensburger,Eva Teufel,Julia Mersi,Max Bittrich,Franziska Seifert,Malik S Haider,Leo Rasche,Jost Hillenkamp,Hermann Einsele,Daniel Kampik,K Martin Kortüm,Johannes M Waldschmidt

Frontiers in immunology 12:647873 PubMed33828557

2021

A Systems Biology Approach Identifies B Cell Maturation Antigen (BCMA) as a Biomarker Reflecting Oral Vaccine Induced IgA Antibody Responses in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Lynda Mottram,Anna Lundgren,Ann-Mari Svennerholm,Susannah Leach
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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