Human BCMA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human BCMA in Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Frontiers in immunology 12:6478732021A Systems Biology Approach Identifies B Cell Maturation Antigen (BCMA) as a Biomarker Reflecting Oral Vaccine Induced IgA Antibody Responses in Humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLynda Mottram,Anna Lundgren,Ann-Mari Svennerholm,Susannah LeachPubMed 33828557
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 10.81 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
Alternative names
CD269, BCM, BCMA, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein
What's included?
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Human BCMA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human BCMA in Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 10.81 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 8.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 109 | Range 107 - 112 % |
Sample type Serum | Average % = 103 | Range 99 - 110 % |
Sample type EDTA Plasma | Average % = 111 | Range 102 - 120 % |
Sample type Urine | Average % = 109 | Range 101 - 116 % |
Sample type Cell culture media | Average % = 96 | Range 87 - 104 % |
Sample type Heparin Plasma | Average % = 107 | Range 105 - 112 % |
Sample type Citrate plasma | Average % = 105 | Range 101 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human BCMA ELISA Kit (ab263875) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BCMA protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BCMA with 10.81 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tumor necrosis factor receptor superfamily member 17 (BCMA) is a receptor involved in B cell survival. BCMA activates the NF-kappa-B and JNK pathways. Aberrant expression of BCMA is observed on malignant B cells, including during multiple myeloma, Hodgkin's lymphoma, and Waldenstrom's macroglobulinemia.
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4 product images
Sandwich ELISA - Human BCMA ELISA Kit (ab263875)
Example of human BCMA standard curve in Sample Diluent NS.
The BCMA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human BCMA ELISA Kit (ab263875)
Interpolated concentrations of native BCMA in human serum and plasma samples.
The concentrations of BCMA were measured in duplicates, interpolated from the BCMA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 25.96 ng/mL in serum, 17.77 ng/mL in plasma (citrate), 18.02 ng/ml in plasma (heparin), and 17.02 ng/mL in plasma (EDTA).
Sandwich ELISA - Human BCMA ELISA Kit (ab263875)
Interpolated concentrations of native BCMA in human cell culture supernatant and pooled-sex urine samples.
The concentrations of BCMA were measured in duplicates, interpolated from the BCMA standard curves and corrected for sample dilution. Undiluted samples are as follows: U266B1 supernatant 1: 200 and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 142.48 ng/mL in U266B1 supernatant and 3.41 ng/mL in pooled-sex urine.
Sandwich ELISA - Human BCMA ELISA Kit (ab263875)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 13.58 ng/mL with a range of 9.70 – 18.54 ng/mL.
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