Human BD-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BD-3 with a sensitivity of 14.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 14.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
Alternative names
BD3, DEFB103, DEFB3, DEFB103B, DEFB103A, Beta-defensin 103, Beta-defensin 3, Defensin-like protein, BD-3, DEFB-3, HBD3, hBD-3
What's included?
Recommended products
Human BD-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BD-3 with a sensitivity of 14.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 14.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 92 - 100 % |
Sample type Citrate plasma | Average % = 95 | Range 91 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human BD-3 ELISA kit (ab272471) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BD-3 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BD-3 with 14.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Defensins are cysteine rich cationic peptides known to exhibit strong antimicrobial and immune signaling activities. BD-3, encoded by the DEFB103A gene, is expressed in many epithelial cells and induced by cytokines or microbial stimuli. Studies shows that BD-3 may play an important role in the innate epithelial defense and wound healing.
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2 product images
Sandwich ELISA - Human BD-3 ELISA Kit (ab272471)
Example of human BD-3 standard curve in Sample Diluent NS.
The BD-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human BD-3 ELISA Kit (ab272471)
Interpolated concentration of spike BD-3 in human serum and plasma samples.
The concentration of BD-3 were measured in duplicates, interpolated from the BD-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5% and plasma (citrate) 12.5%. The interpolated values are plotted (mean +/- SD, n=2).
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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