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AB272471

Human BD-3 ELISA Kit

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Human BD-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BD-3 with a sensitivity of 14.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BD3, DEFB103, DEFB3, DEFB103B, DEFB103A, Beta-defensin 103, Beta-defensin 3, Defensin-like protein, BD-3, DEFB-3, HBD3, hBD-3

2 Images
Sandwich ELISA - Human BD-3 ELISA Kit (AB272471)
  • sELISA

Supplier Data

Sandwich ELISA - Human BD-3 ELISA Kit (AB272471)

Example of human BD-3 standard curve in Sample Diluent NS.

The BD-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human BD-3 ELISA Kit (AB272471)
  • sELISA

Supplier Data

Sandwich ELISA - Human BD-3 ELISA Kit (AB272471)

Interpolated concentration of spike BD-3 in human serum and plasma samples.

The concentration of BD-3 were measured in duplicates, interpolated from the BD-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5% and plasma (citrate) 12.5%. The interpolated values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14.3 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human BD-3 ELISA kit (ab272471) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BD-3 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BD-3 with 14.3 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Defensins are cysteine rich cationic peptides known to exhibit strong antimicrobial and immune signaling activities. BD-3, encoded by the DEFB103A gene, is expressed in many epithelial cells and induced by cytokines or microbial stimuli. Studies shows that BD-3 may play an important role in the innate epithelial defense and wound healing.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 100 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "91 - 99 %", "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
See full target information DEFB103A
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com