JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB212166

Human BDNF ELISA Kit

4

(2 Reviews)

|

(43 Publications)

Human BDNF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify BDNF with a sensitivity of 2.4 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric assay – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications- Broad sample compatibility – works with serum, plasma, cell culture supernatants, and cerebrospinal fluid- Cited in over 30 publications

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF

7 Images
ELISA - Human BDNF ELISA Kit (AB212166)
  • ELISA

Supplier Data

ELISA - Human BDNF ELISA Kit (AB212166)
Sandwich ELISA - Human BDNF ELISA Kit (AB212166)
  • sELISA

Supplier Data

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)

Serum from ten individual healthy human male and female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 1,176 pg/mL with a range of 653 – 2,387 pg/mL.

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)
  • sELISA

Supplier Data

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)

Example of human BDNF standard curve in Sample Diluent NS.

The BDNF standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)
  • sELISA

Supplier Data

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)

Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.

L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. BDNF was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the BDNF Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 2.3 pg/mL unstimulated, 50 pg/mL stimulated, and undetectable in media.

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)
  • sELISA

Supplier Data

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)

Interpolated concentrations of native BDNF in rat serum sample.

The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 2,471 pg/mL in serum.

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)
  • sELISA

Supplier Data

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)

Example of human BDNF standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)
  • sELISA

Supplier Data

Sandwich ELISA - Human BDNF ELISA Kit (AB212166)

Interpolated concentrations of native BDNF in human serum, and plasma samples.

The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 954 pg/mL in serum, 2,189 pg/mL in plasma (citrate), 690 pg/mL in plasma (EDTA) and 856 pg/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.4 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB108319

Anti-BDNF antibody [EPR1292]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-BDNF antibody [EPR1292] (AB108319)

  • 4
  • 9
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human BDNF ELISA Kit ab212166 is a rapid single-wash 90-min ELISA kit to measure Human BDNF in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 2.4 pg/mL.

How the assay works
Human BDNF SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Human BDNF ELISA Kit ab212166 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human BDNF ELISA Kit ab212166
Human BDNF ELISA Kit ab212166 has been used in publications to study intermittent hypoxia training as a prevention for learning-memory decline in Alzheimer's Disease(1), the association between BDNF levels and postural instability and gait disturbance in Parkinson's Disease(2), and the effects of alcohol on BDNF blood levels(3).

References:
(1)Xiangrong Shi et al. 2021 PMID: 34276338,
(2)Chien Tai Hong et al. 2021 PMID: 32932791,
(3)Amirhesam Alirezaei et al. 2023 PMID: 37845289

Related and recommended products
Human BDNF ELISA Kit ab212166 is commonly used to study neural health.

Other kits for measuring the survival, growth, and differentiation of neurons include:
- Human Agrin ELISA Kit ab216945
- Human IL-6 ELISA kit ab178013

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
style
left-column

Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "88 - 104 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "103 - 109 %", "average": "= 106" }, { "sample": "Serum", "range": "94 - 104 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "85 - 108 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "96 - 112 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "95 - 111 %", "average": "= 103" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab212166-10x96Tests", "assetComponentDetails": [ { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB212166-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB212166-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB212166-CMP04" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB212166-CMP06" }, { "size":"10 x 600 µL", "name":"10X Human BDNF Detector Antibody", "number":"AB212166-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB212166-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB212166-CMP09" }, { "size":"20 x 1 Vial", "name":"Human BDNF Lyophilized Recombinant Protein", "number":"AB212166-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human BDNF Capture Antibody", "number":"AB212166-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB212166-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab212166-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB212166-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB212166-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB212166-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB212166-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BDNF Capture Antibody", "number":"AB212166-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human BDNF Lyophilized Recombinant Protein", "number":"AB212166-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB212166-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human BDNF Detector Antibody", "number":"AB212166-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB212166-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB212166-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed : 11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed : 24908487, PubMed : 29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
See full target information BDNF
style
left-column

Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroscience research 103:e70058 PubMed40552481

2025

Concomitant Increases in Brain-Derived Neurotrophic Factor and Lactate Post-Exercise Do Not Demonstrate a Direct Correlation.

Applications

Unspecified application

Species

Unspecified reactive species

S K Ashcroft,K Basclain,C Woolnough,M W Hoon,S J Walsh,L C Starc,L Johnson,S S Kuys,A G Thompson-Butel

American journal of translational research 17:3198-3206 PubMed40384998

2025

The effect of an intravenous analgesic pump with esketamine on postoperative pain and postpartum depression in women with cesarean section.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Xiao,Baoyi Su,Zhuoyu You,Hui Lu,Tuanming Huang

Frontiers in endocrinology 16:1551172 PubMed40375951

2025

Neuroendocrine characterization into schizophrenia: norepinephrine and melatonin as promising biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Shen,Xin Li,Yinghua Zhong,Jiechun Zhang,Hongyun Qin,Fazhan Chen,Xudong Zhao

Journal of nanobiotechnology 23:209 PubMed40075449

2025

Development of NIR photocleavable nanoparticles with BDNF for vestibular neuron regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Celine Dg Abueva,Sung Ryeong Yoon,Nathaniel T Carpena,Seung Cheol Ahn,So-Young Chang,Ji Eun Choi,Min Young Lee,Jae Yun Jung

Future science OA 11:2471723 PubMed40035430

2025

Biodistribution and persistence of human umbilical cord-derived mesenchymal stem cells in NCG mice: a comparative study.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyang Liu,Herui Wang,Xin Li,Yi Mi,Chenliang Zhang,Yaoyao Chen,Li Miao,Haomiao Long,Jun He,Qinggang Ge,Yongjun Liu

Molecular therapy. Methods & clinical development 33:101415 PubMed39995448

2025

Human striatal progenitor cells that contain inducible safeguards and overexpress BDNF rescue Huntington's disease phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle A Simmons,Sridhar Selvaraj,Tingshuo Chen,Gloria Cao,Talita Souto Camelo,Tyne L M McHugh,Selena Gonzalez,Renata M Martin,Juste Simanauskaite,Nobuko Uchida,Matthew H Porteus,Frank M Longo

RNA (New York, N.Y.) 30:1554-1571 PubMed39379106

2024

Site-blocking antisense oligonucleotides as a mechanism to fine-tune MeCP2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda M Vanderplow,Grace E Dodis,Yewon Rhee,Jakub J Cikowski,Sonia Gonzalez,Mackenzie L Smith,Rocco G Gogliotti

Sports (Basel, Switzerland) 12: PubMed39195599

2024

Changes in Physical Fitness, Muscle Damage and Cognitive Function in Elite Rugby Players over a Season.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Houssem Karamti,Hassane Zouhal,Mariem Bousselmi,Manel Darragi,Hamdi Khannous,Ahlem Ben Hmid,Imen Zamali,Mélika Ben Ahmed,Ismail Laher,Urs Granacher,Amira Zouita Ben Moussa

Cell biology international 48:1698-1713 PubMed39080995

2024

Overexpression of EGFL7 promotes angiogenesis and nerve regeneration in peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zengtao Hao,Zhiqi Huo,Qicheng Aixin-Jueluo,Tao Wu,Yihong Chen

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 58:292-310 PubMed38973197

2024

Role of Piezo2 in Schwann Cell Volume Regulation and Its Impact on Neurotrophic Release Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chawapun Suttinont,Katsuyuki Maeno,Mamiko Yano,Kaori Sato-Numata,Tomohiro Numata,Moe Tsutsumi
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com