Human BDNF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify BDNF with a sensitivity of 2.4 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric assay – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications- Broad sample compatibility – works with serum, plasma, cell culture supernatants, and cerebrospinal fluid- Cited in over 30 publications
Images
Publications
Filter by
- Biomolecules & biomedicine 24:520-5362024Exosome miR-4738-3p-mediated regulation of through the NF-κB and inflammation signaling pathway alleviates osteoarthritis low-grade inflammation symptoms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Xu,Kaifeng Zhou,Huijie Gu,Yiming Zhang,Liang Wu,Chong Bian,Zhongyue Huang,Guangnan Chen,Xiangyang Cheng,Xiaofan YinPubMed 38059912
- Scientific reports 13:175542023Effect of alcohol on Brain-Derived Neurotrophic Factor (BDNF) blood levels: a systematic review and meta-analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArman Shafiee,Kyana Jafarabady,Mohammad Ali Rafiei,Maryam Beiky,Niloofar Seighali,Golshid Golpayegani,Mehrsa Jalali,Faeze Soltani Abhari,Razman Arabzadeh Bahri,Omid Safari,Mahmood Bakhtiyari,Amirhesam AlirezaeiPubMed 37845289
- Glia 72:133-1552023Human iPSC-derived endothelial cells promote CNS remyelination via BDNF and mTORC1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Ma,Huiyuan Zhang,Le Yin,Hao Xu,Lida Wu,Rahul Shaji,Fatema Rezai,Ayesha Mulla,Sukhteerath Kaur,Shengjiang Tan,Boris Kysela,Yilong Wang,Zhiguo Chen,Chao Zhao,Yuchun GuPubMed 37675625
- Nutrients 15:2023A Randomized, Double-Blind, Placebo-Controlled Study of an Anthocyanin-Rich Functional Ingredient on Cognitive Function and Eye Dryness in Late Adulthood Volunteers: Roles of Epigenetic and Gut Microbiome Modulations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJintanaporn Wattanathorn,Terdthai Tong-Un,Wipawee Thukham-Mee,Pongsatorn Paholpak,Poonsri RangseekhajeePubMed 37630690
- International journal of molecular sciences 24:2023Experimental Treatment with Edaravone in a Mouse Model of Spinocerebellar Ataxia 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Sucha,Simona Benediktova,Filip Tichanek,Jan Jedlicka,Stepan Kapl,Dana Jelinkova,Zdenka Purkartova,Jan Tuma,Jitka Kuncova,Jan CendelinPubMed 37445867
- The American journal of clinical nutrition 118:369-3812023A combined DHA-rich fish oil and cocoa flavanols intervention does not improve cognition or brain structure in older adults with memory complaints: results from the CANN randomized, controlled parallel-design study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Vauzour,Andrew Scholey,David J White,Neal J Cohen,Aedín Cassidy,Rachel Gillings,Michael A Irvine,Colin D Kay,Min Kim,Rebecca King,Cristina Legido-Quigley,John F Potter,Hilary Schwarb,Anne-Marie MinihanePubMed 37315924
- International journal of molecular sciences 24:2023Intraocular Pressure-Lowering and Retina-Protective Effects of Exosome-Rich Conditioned Media from Human Amniotic Membrane Stem Cells in a Rat Model of Glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye-Rim Seong,Chan Ho Noh,Sangryong Park,Sumin Cho,Seok-Jin Hong,Ah-Young Lee,Dongho Geum,Soon-Cheol Hong,Dongsun Park,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae KimPubMed 37175778
- International journal of molecular sciences 24:2023Insomnia Impairs Both the Pro-BDNF and the BDNF Levels Similarly to Older Adults with Cognitive Decline: An Exploratory Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergio Sánchez-García,Karla Moreno-Tamayo,Ricardo Ramírez-Aldana,Carmen García-Peña,Raúl Hernán Medina-Campos,Paola García Dela Torre,Nadia Alejandra Rivero-SeguraPubMed 37108547
- Frontiers in molecular neuroscience 15:10756132023Development and validation of brain-derived neurotrophic factor measurement in human urine samples as a non-invasive effect biomarker.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlicia Olivas-Martinez,Beatriz Suarez,Elena Salamanca-Fernandez,Iris Reina-Perez,Andrea Rodriguez-Carrillo,Vicente Mustieles,Nicolás Olea,Carmen Freire,Mariana F FernándezPubMed 36710936
- Journal of clinical laboratory analysis 37:e248032022Serum brain-derived neurotrophic factor in coronary heart disease: Correlation with the T helper (Th)1/Th2 ratio, Th17/regulatory T (Treg) ratio, and major adverse cardiovascular events.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanfei Huo,Qiang Feng,Jie Fan,Jing Huang,Yanling Zhu,Yanqiang Wu,Aijun Hou,Lin ZhuPubMed 36510348
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.4 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
The protein expressed by the BDNF gene is a critical signaling molecule that activates pathways downstream of NTRK2, as well as the heterodimeric receptor formed by NGFR and SORCS2. During development, it aids the survival and differentiation of specific neuronal populations in both the peripheral and central nervous systems. It is involved in axonal growth, pathfinding, and modulation of dendritic growth and morphology. In adult synapses, BDNF is a key regulator of synaptic transmission and plasticity in various CNS regions, contributing to processes like long-term potentiation, long-term depression, some short-term synaptic plasticity forms, and the homeostatic regulation of intrinsic neuronal excitability. Signaling through NGFR and SORCS2 is linked to synaptic plasticity and long-term depression, while its binding to these receptors also promotes neuronal apoptosis. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF
What's included?
Recommended products
Human BDNF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify BDNF with a sensitivity of 2.4 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric assay – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications- Broad sample compatibility – works with serum, plasma, cell culture supernatants, and cerebrospinal fluid- Cited in over 30 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.4 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 5 | C.V. 2.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 5.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cerebral Spinal Fluid | Average % = 95 | Range 88 - 104 % |
Sample type Cell culture supernatant | Average % = 106 | Range 103 - 109 % |
Sample type Serum | Average % = 97 | Range 94 - 104 % |
Sample type EDTA Plasma | Average % = 98 | Range 85 - 108 % |
Sample type Heparin Plasma | Average % = 103 | Range 96 - 112 % |
Sample type Citrate plasma | Average % = 103 | Range 95 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human BDNF ELISA Kit ab212166 is a rapid single-wash 90-min ELISA kit to measure Human BDNF in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 2.4 pg/mL.
How the assay works
Human BDNF SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
Human BDNF ELISA Kit ab212166 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human BDNF ELISA Kit ab212166
Human BDNF ELISA Kit ab212166 has been used in publications to study intermittent hypoxia training as a prevention for learning-memory decline in Alzheimer's Disease(1), the association between BDNF levels and postural instability and gait disturbance in Parkinson's Disease(2), and the effects of alcohol on BDNF blood levels(3).
References:
(1)Xiangrong Shi et al. 2021 PMID: 34276338,(2)Chien Tai Hong et al. 2021 PMID: 32932791,
(3)Amirhesam Alirezaei et al. 2023 PMID: 37845289
Related and recommended products
Human BDNF ELISA Kit ab212166 is commonly used to study neural health.
Other kits for measuring the survival, growth, and differentiation of neurons include:
- Human Agrin ELISA Kit Human Agrin ELISA Kit ab216945
- Human IL-6 ELISA kit Human IL-6 ELISA Kit ab178013
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Human BDNF ELISA Kit (ab212166)
Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. BDNF was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the BDNF Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 2.3 pg/mL unstimulated, 50 pg/mL stimulated, and undetectable in media.
Sandwich ELISA - Human BDNF ELISA Kit (ab212166)
Example of human BDNF standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human BDNF ELISA Kit (ab212166)
Interpolated concentrations of native BDNF in human serum, and plasma samples.
The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 954 pg/mL in serum, 2,189 pg/mL in plasma (citrate), 690 pg/mL in plasma (EDTA) and 856 pg/mL in plasma (heparin).
Sandwich ELISA - Human BDNF ELISA Kit (ab212166)
Serum from ten individual healthy human male and female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 1,176 pg/mL with a range of 653 – 2,387 pg/mL.
Sandwich ELISA - Human BDNF ELISA Kit (ab212166)
Interpolated concentrations of native BDNF in rat serum sample.
The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 2,471 pg/mL in serum.
Sandwich ELISA - Human BDNF ELISA Kit (ab212166)
Example of human BDNF standard curve in Sample Diluent NS.
The BDNF standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com