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The Human BDNF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of brain-derived neurotrophic factor (BDNF) levels with a sensitivity of 2.4 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive human proBDNF sandwich ELISA kit:- Colorimetric assay – 450 nm readout, compatible with any standard plate reader- Available in different formats – 384-well format available for high-throughput applications- Broad sample compatibility – works with serum, plasma (heparin, citrate, EDTA), cell culture supernatants, and cerebrospinal fluid- Cited in over 30 publications


Images

Sandwich ELISA - Human BDNF ELISA Kit (AB212166), expandable thumbnail
  • Sandwich ELISA - Human BDNF ELISA Kit (AB212166), expandable thumbnail
  • Sandwich ELISA - Human BDNF ELISA Kit (AB212166), expandable thumbnail
  • Sandwich ELISA - Human BDNF ELISA Kit (AB212166), expandable thumbnail
  • Sandwich ELISA - Human BDNF ELISA Kit (AB212166), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Sensitivity

= 2.4 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.Neurotrophic factor BDNF precursor formImportant signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human BDNF Capture Antibody
1 x 600 µL
10X Human BDNF Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Human BDNF Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

The Human BDNF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of brain-derived neurotrophic factor (BDNF) levels with a sensitivity of 2.4 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive human proBDNF sandwich ELISA kit:- Colorimetric assay – 450 nm readout, compatible with any standard plate reader- Available in different formats – 384-well format available for high-throughput applications- Broad sample compatibility – works with serum, plasma (heparin, citrate, EDTA), cell culture supernatants, and cerebrospinal fluid- Cited in over 30 publications

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 2.4 pg/mL

Precision

Intra assay

Sample

Serum

n
5
mean
-
SD
-
C.V.

2.8

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

5.3

Recovery

Sample specific recovery

Sample type

Cerebral Spinal Fluid

Average %

= 95

Range

88 - 104 %

Sample type

Cell culture supernatant

Average %

= 106

Range

103 - 109 %

Sample type

Serum

Average %

= 97

Range

94 - 104 %

Sample type

EDTA Plasma

Average %

= 98

Range

85 - 108 %

Sample type

Heparin Plasma

Average %

= 103

Range

96 - 112 %

Sample type

Citrate plasma

Average %

= 103

Range

95 - 111 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human BDNF ELISA Kit (ab212166) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BDNF protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BDNF with 2.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SPECIES REACTIVITY

This kit recognizes human BDNF protein and is cross-reactive to mouse and rat BDNF protein.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only.

Cell and tissue extract samples have not been tested with this kit.

CROSS REACTIVITY

Recombinant TRKB was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.

INTERFERENCE

Recombinant TRKB was prepared at 50 ng/mL and 1 ng/mL and tested for interference with human BDNF at 200 pg/mL. 39% Inference was observed with 1ng/mL and 76% Interference was observed with 50 ng/mL.

BDNF (Brain derived neurotrophic factor) is a member of the neurotrophin family of growth factors that includes nerve growth factor. BDNF is expressed as a proprotein that is cleaved to form mature BDNF (residues 129-247). This assay measures mature BDNF. Mature BDNF protein sequence is identical between human, mouse, rat and many additional species. BDNF binds to at least 2 receptors: TrkB and LNGFR.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Sandwich ELISA - Human BDNF ELISA Kit (ab212166), expandable thumbnail

    Sandwich ELISA - Human BDNF ELISA Kit (ab212166)

    Example of human BDNF standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human BDNF ELISA Kit (ab212166), expandable thumbnail

    Sandwich ELISA - Human BDNF ELISA Kit (ab212166)

    Interpolated concentrations of native BDNF in human serum, and plasma samples.

    The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 954 pg/mL in serum, 2,189 pg/mL in plasma (citrate), 690 pg/mL in plasma (EDTA) and 856 pg/mL in plasma (heparin).

  • Sandwich ELISA - Human BDNF ELISA Kit (ab212166), expandable thumbnail

    Sandwich ELISA - Human BDNF ELISA Kit (ab212166)

    Serum from ten individual healthy human male and female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 1,176 pg/mL with a range of 653 – 2,387 pg/mL.

  • Sandwich ELISA - Human BDNF ELISA Kit (ab212166), expandable thumbnail

    Sandwich ELISA - Human BDNF ELISA Kit (ab212166)

    Interpolated concentrations of native BDNF in rat serum sample.

    The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 2,471 pg/mL in serum.

  • Sandwich ELISA - Human BDNF ELISA Kit (ab212166), expandable thumbnail

    Sandwich ELISA - Human BDNF ELISA Kit (ab212166)

    Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.

    L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. BDNF was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the BDNF Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 2.3 pg/mL unstimulated, 50 pg/mL stimulated, and undetectable in media.

  • Sandwich ELISA - Human BDNF ELISA Kit (ab212166), expandable thumbnail

    Sandwich ELISA - Human BDNF ELISA Kit (ab212166)

    Example of human BDNF standard curve in Sample Diluent NS.

    The BDNF standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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