AB229395

Human BDNF ELISA Kit, Fluorescent

CatchPoint SimpleStep ELISA
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(1 Publication)

Human BDNF ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human BDNF with a sensitivity of 1.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF

5 Images

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

sELISA

Unknown

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Interpolated concentrations of native BDNF in human serum, and plasma samples.

The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 954 pg/mL in serum, 2,189 pg/mL in plasma (citrate), 690 pg/mL in plasma (EDTA) and 856 pg/mL in plasma (heparin).

sELISA

Unknown

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Interpolated concentrations of native BDNF in rat serum sample.

The concentrations of BDNF were measured in duplicates, interpolated from the BDNF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 2,471 pg/mL in serum.

sELISA

Unknown

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Serum from ten individual healthy human male and female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BDNF concentration was determined to be 1,176 pg/mL with a range of 653 – 2,387 pg/mL.

sELISA

Unknown

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Human BDNF standard curve in Sample Diluent NS.

The BDNF standard curve was prepared as described in Section 10.

sELISA

Unknown

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Comparison of secreted BDNF in unstimulated and PMA/PHA-stimulated L929 Cells.

L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. BDNF was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the BDNF Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 2.3 pg/mL unstimulated, 50 pg/mL stimulated, and undetectable in media.

Key facts

Detection method

Fluorescent

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.5 pg/mL

Range

2 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

BDNF in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BDNF protein in human serum, plasma, and cell culture supernatant.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

BDNF (brain derived neurotrophic factor) is a member of the neurotrophin family of growth factors that includes nerve growth factor. BDNF is expressed as a proprotein that is cleaved to form mature BDNF (residues 129-247). This assay measures mature BDNF. Mature BDNF protein sequence is identical between human, mouse, rat and many additional species. BDNF binds to at least 2 receptors: TrkB and LNGFR.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "88 - 104 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "103 - 109 %", "average": "= 106" }, { "sample": "Serum", "range": "94 - 104 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "85 - 108 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "96 - 112 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "95 - 111 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed : 11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed : 24908487, PubMed : 29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).

See full target information BDNF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Ger 6:1900520 PubMed31453065

2019

Adaptable Microporous Hydrogels of Propagating NGF-Gradient by Injectable Building Blocks for Accelerated Axonal Outgrowth.

Applications

Unspecified application

Species

Unspecified reactive species

Ru-Siou Hsu,Pei-Yueh Chen,Jen-Hung Fang,You-Yin Chen,Chien-Wen Chang,Yu-Jen Lu,Shang-Hsiu Hu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human BDNF ELISA Kit, Fluorescent

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Sandwich ELISA - Human BDNF ELISA Kit, Fluorescent (AB229395)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise