AB254511

Human Beclin-1 ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Human Beclin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Beclin-1 with a sensitivity of 671 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

3 Images

ELISA - Human Beclin-1 ELISA Kit (AB254511)

ELISA

Supplier Data

ELISA - Human Beclin-1 ELISA Kit (AB254511)

sELISA

Supplier Data

Sandwich ELISA - Human Beclin-1 ELISA Kit (AB254511)

Example of human Beclin-1 standard curve in 1X Cell Extraction Buffer PTR.

The Beclin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Beclin-1 ELISA Kit (AB254511)

Interpolated concentrations of native Beclin-1 in human MCF-7 cell extract based on a 1,000 μg/mL extract load.

The concentrations of Beclin-1 were measured in duplicate and interpolated from the Beclin-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beclin-1 concentration was determined to be 10.03 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 671 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Beclin-1 ELISA Kit ab254511 is a rapid single-wash 90-min Sandwich ELISA to measure Human Beclin-1 in cell culture extracts. This SimpleStep sensitivity is 671 pg/mL.

How the assay works

Human Beclin-1 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Beclin-1 ELISA Kit ab254511 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Beclin 1 antibody [EPR20473-245] - BSA and Azide free (Capture) ab252668
- Anti-Beclin 1 antibody [EPR20473-221] - BSA and Azide free (Detector) ab252668

Beclin-1, also known as BECN1, plays a key role in the regulation of autophagy and cell death. Together with PI3KC3, Beclin-1 nucleates autophagosome formation and is essential for proper degradation of cellular components. Improper beclin-1 signaling is associated with schizophrenia, tumorigenesis, and neurodegeneration.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 117 %", "average": "= 109" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

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Product protocols

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Target data

Plays a central role in autophagy (PubMed : 18570871, PubMed : 21358617, PubMed : 23184933, PubMed : 23974797, PubMed : 25484083, PubMed : 28445460, PubMed : 37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123, PubMed : 23974797, PubMed : 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed : 25275521). May play a role in antiviral host defense.. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

See full target information BECN1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:7024 PubMed40016261

2025

NLRP3 overexpression exacerbated synovium tissue degeneration in juvenile collagen-induced arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Fater A Khadour,Younes A Khadour,Tao Xu

Cells 12: PubMed37174682

2023

Autophagy Receptor p62 Regulates SARS-CoV-2-Induced Inflammation in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Verica Paunovic,Ljubica Vucicevic,Maja Misirkic Marjanovic,Vladimir Perovic,Biljana Ristic,Mihajlo Bosnjak,Milos Mandic,Danijela Stevanovic,Ljubica Harhaji-Trajkovic,Jovan Lalosevic,Milos Nikolic,Branka Bonaci-Nikolic,Vladimir Trajkovic

American journal of translational research 14:6056-6066 PubMed36247304

2022

Changes of serum P62 and Beclin 1 in patients with pulmonary embolism during treatment and their predictive value for efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehui Zhang,Liu Huang,Lijiang Shao,Xuxia Chen,Jinguo Chu,Xueqin Chen

Experimental and therapeutic medicine 23:267 PubMed35251333

2022

Sirtuin 1 participates in intervertebral disc degeneration via the nicotinamide phosphoribosyl transferase/nicotinamide adenine dinucleotide/sirtuin 1 pathway responsible for regulating autophagy of nucleus pulposus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qifeng Shi,Liang Xu,Xiangyi Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Beclin-1 ELISA Kit

ELISA - Human Beclin-1 ELISA Kit (AB254511)

Sandwich ELISA - Human Beclin-1 ELISA Kit (AB254511)

Sandwich ELISA - Human Beclin-1 ELISA Kit (AB254511)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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