Human Beclin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Beclin-1 with a sensitivity of 671 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Publications
Filter by
- Cells 12:2023Autophagy Receptor p62 Regulates SARS-CoV-2-Induced Inflammation in COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVerica Paunovic,Ljubica Vucicevic,Maja Misirkic Marjanovic,Vladimir Perovic,Biljana Ristic,Mihajlo Bosnjak,Milos Mandic,Danijela Stevanovic,Ljubica Harhaji-Trajkovic,Jovan Lalosevic,Milos Nikolic,Branka Bonaci-Nikolic,Vladimir TrajkovicPubMed 37174682
- American journal of translational research 14:6056-60662022Changes of serum P62 and Beclin 1 in patients with pulmonary embolism during treatment and their predictive value for efficacy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuehui Zhang,Liu Huang,Lijiang Shao,Xuxia Chen,Jinguo Chu,Xueqin ChenPubMed 36247304
- Experimental and therapeutic medicine 23:2672022Sirtuin 1 participates in intervertebral disc degeneration via the nicotinamide phosphoribosyl transferase/nicotinamide adenine dinucleotide/sirtuin 1 pathway responsible for regulating autophagy of nucleus pulposus cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQifeng Shi,Liang Xu,Xiangyi ZengPubMed 35251333
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay time
- 1h 30m
- Sensitivity
- = 671 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
Alternative names
GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
What's included?
Recommended products
Human Beclin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Beclin-1 with a sensitivity of 671 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 671 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 24 | C.V. 5.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 1.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 109 | Range 99 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Beclin-1 ELISA Kit ab254511 is a rapid single-wash 90-min Sandwich ELISA to measure Human Beclin-1 in cell culture extracts. This SimpleStep sensitivity is 671 pg/mL.
How the assay works
Human Beclin-1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Beclin-1 ELISA Kit ab254511 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Beclin 1 antibody [EPR20473-245] - BSA and Azide free (Capture) Anti-Beclin 1 antibody [EPR20473-221] - BSA and Azide free (Detector) ab252668
- Anti-Beclin 1 antibody [EPR20473-221] - BSA and Azide free (Detector) Anti-Beclin 1 antibody [EPR20473-221] - BSA and Azide free (Detector) ab252668
Beclin-1, also known as BECN1, plays a key role in the regulation of autophagy and cell death. Together with PI3KC3, Beclin-1 nucleates autophagosome formation and is essential for proper degradation of cellular components. Improper beclin-1 signaling is associated with schizophrenia, tumorigenesis, and neurodegeneration.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.
- Biological function summary
The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.
- Pathways
Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.
- Associated diseases and disorders
Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
ELISA - Human Beclin-1 ELISA Kit (ab254511)
Sandwich ELISA - Human Beclin-1 ELISA Kit (ab254511)
Example of human Beclin-1 standard curve in 1X Cell Extraction Buffer PTR.
The Beclin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Beclin-1 ELISA Kit (ab254511)
Interpolated concentrations of native Beclin-1 in human MCF-7 cell extract based on a 1,000 μg/mL extract load.
The concentrations of Beclin-1 were measured in duplicate and interpolated from the Beclin-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beclin-1 concentration was determined to be 10.03 ng/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com