Human Beclin-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Beclin-1 in Cell culture extracts samples.
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- Cells 12:2023Autophagy Receptor p62 Regulates SARS-CoV-2-Induced Inflammation in COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVerica Paunovic,Ljubica Vucicevic,Maja Misirkic Marjanovic,Vladimir Perovic,Biljana Ristic,Mihajlo Bosnjak,Milos Mandic,Danijela Stevanovic,Ljubica Harhaji-Trajkovic,Jovan Lalosevic,Milos Nikolic,Branka Bonaci-Nikolic,Vladimir TrajkovicPubMed 37174682
- American journal of translational research 14:6056-60662022Changes of serum P62 and Beclin 1 in patients with pulmonary embolism during treatment and their predictive value for efficacy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuehui Zhang,Liu Huang,Lijiang Shao,Xuxia Chen,Jinguo Chu,Xueqin ChenPubMed 36247304
- Experimental and therapeutic medicine 23:2672022Sirtuin 1 participates in intervertebral disc degeneration via the nicotinamide phosphoribosyl transferase/nicotinamide adenine dinucleotide/sirtuin 1 pathway responsible for regulating autophagy of nucleus pulposus cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQifeng Shi,Liang Xu,Xiangyi ZengPubMed 35251333
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay time
- 1h 30m
- Sensitivity
- = 671 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
Alternative names
GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
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Human Beclin-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Beclin-1 in Cell culture extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 671 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 24 | mean - | SD - | C.V. 5.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 1.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 109 | Range 99 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Beclin-1 ELISA Kit (ab254511) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beclin-1 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Beclin-1 with 671 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Beclin-1, also known as BECN1, plays a key role in the regulation of autophagy and cell death. Together with PI3KC3, Beclin-1 nucleates autophagosome formation and is essential for proper degradation of cellular components. Improper beclin-1 signaling is associated with schizophrenia, tumorigenesis, and neurodegeneration.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.
- Biological function summary
The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.
- Pathways
Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.
- Associated diseases and disorders
Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.
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2 product images
Sandwich ELISA - Human Beclin-1 ELISA Kit (ab254511)
Example of human Beclin-1 standard curve in 1X Cell Extraction Buffer PTR.
The Beclin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Beclin-1 ELISA Kit (ab254511)
Interpolated concentrations of native Beclin-1 in human MCF-7 cell extract based on a 1,000 μg/mL extract load.
The concentrations of Beclin-1 were measured in duplicate and interpolated from the Beclin-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beclin-1 concentration was determined to be 10.03 ng/mL.
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