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AB181423

Human Beta-2-Microglobulin ELISA Kit

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(2 Publications)

Human Beta-2-Microglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Beta-2-Microglobulin with a sensitivity of 21.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

6 Images
ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)
  • ELISA

Supplier Data

ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)
Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)
  • sELISA

Supplier Data

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)

Interpolated concentrations of native beta-2 microglobulin in human serum, plasma, and urine samples.

The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400, plasma (citrate) 1 : 200, plasma (EDTA) 1 : 400, plasma (heparin) 1 : 200, and 1 : 400 urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1037.2 ng/mL in serum, 657.5 ng/mL in plasma (citrate), 1039.9 ng/mL in plasma (EDTA), 787.5 ng/mL in plasma (heparin), and 1348.2 ng/mL in urine.

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)
  • sELISA

Supplier Data

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1093.9 ng/mL with a range of 727.2 – 1537.0 ng/mL.

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)
  • sELISA

Unknown

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)

Example of human beta-2 microglobulin standard curve in Sample Diluent NS.

The beta-2 microglobulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)
  • sELISA

Supplier Data

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)

Interpolated concentrations of native beta-2 microglobulin in human HepG2 cell culture supernatant sample.

The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 1 : 32. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 119.4 ng/mL in HepG2 cell culture supernatant.

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)
  • sELISA

Lab

Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)

Human beta-2-microglobulin standard curve comparison.

Standard Curve comparison between the original Human beta-2-microglobulin SimpleStep ELISA kit and current Human beta-2-microglobulin SimpleStep ELISA kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 21.2 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Beta-2-Microglobulin ELISA Kit ab181423 is a rapid single-wash 90-min Sandwich ELISA to measure Human Beta-2-Microglobulin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, plasma, serum, urine. This SimpleStep sensitivity is 21.2 pg/mL.

How the assay works

Human Beta-2-Microglobulin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Beta-2-Microglobulin ELISA Kit ab181423 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-beta 2 Microglobulin antibody [EPR20040-22] - BSA and Azide free (Capture) ab255614
- Anti-beta 2 Microglobulin antibody [EPR20040-45] - BSA and Azide free (Detector) ab255614

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) involved in the presentation of peptide antigens to the immune system. The full length B2M protein exists as a heterodimer of an alpha chain and a beta chain. Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) where affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation. Persistently high B2M serum levels lead to amyloidosis in patients on long-term hemodialysis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Serum", "range": "90 - 92 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "87 - 89 %", "average": "= 90" }, { "sample": "Urine", "range": "87 - 100 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "88 - 91 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "89 - 91 %", "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab181423-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB181423-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human B2M Lyophilized Recombinant Protein", "number":"AB181423-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB181423-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB181423-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI - HAMA Blocker (ab193969)", "number":"AB181423-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human B2M Capture Antibody", "number":"AB181423-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB181423-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB181423-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB181423-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human B2M Detector Antibody", "number":"AB181423-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
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Product protocols

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).
See full target information B2M
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 18:106 PubMed27180164

2016

Identification of definitive serum biomarkers associated with disease activity in primary Sjögren's syndrome.

Applications

sELISA

Species

Human

Ayumi Nishikawa,Katsuya Suzuki,Yoshiaki Kassai,Yuumi Gotou,Maiko Takiguchi,Takahiro Miyazaki,Keiko Yoshimoto,Hidekata Yasuoka,Kunihiro Yamaoka,Rimpei Morita,Akihiko Yoshimura,Tsutomu Takeuchi

Cancer chemotherapy and pharmacology 76:989-96 PubMed26407820

2015

Urinary kidney injury molecule-1 and monocyte chemotactic protein-1 are noninvasive biomarkers of cisplatin-induced nephrotoxicity in lung cancer patients.

Applications

sELISA

Species

Human

Haruka Shinke,Satohiro Masuda,Yousuke Togashi,Yasuaki Ikemi,Aiko Ozawa,Tomoko Sato,Young Hak Kim,Michiaki Mishima,Takaharu Ichimura,Joseph V Bonventre,Kazuo Matsubara
View all publications
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websiteProtocolBooklet
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