Human Beta-2-Microglobulin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Beta-2-Microglobulin in Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Arthritis research & therapy 18:1062016Identification of definitive serum biomarkers associated with disease activity in primary Sjögren's syndrome.
- Cancer chemotherapy and pharmacology 76:989-962015Urinary kidney injury molecule-1 and monocyte chemotactic protein-1 are noninvasive biomarkers of cisplatin-induced nephrotoxicity in lung cancer patients.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 21.2 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
Alternative names
CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin
What's included?
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Human Beta-2-Microglobulin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Beta-2-Microglobulin in Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 21.2 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 8 | mean - | SD - | C.V. 3.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 3 | mean - | SD - | C.V. 2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 91 | Range 89 - 94 % |
Sample type Serum | Average % = 91 | Range 90 - 92 % |
Sample type EDTA Plasma | Average % = 90 | Range 87 - 89 % |
Sample type Urine | Average % = 94 | Range 87 - 100 % |
Sample type Heparin Plasma | Average % = 90 | Range 88 - 91 % |
Sample type Citrate plasma | Average % = 90 | Range 89 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human B2M ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Beta-2-Microglobulin ELISA kit (ab181423) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of B2M protein in human serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human B2M with 31.25 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) involved in the presentation of peptide antigens to the immune system. The full length B2M protein exists as a heterodimer of an alpha chain and a beta chain. Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) where affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation. Persistently high B2M serum levels lead to amyloidosis in patients on long-term hemodialysis.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
- Biological function summary
Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.
- Pathways
Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.
- Associated diseases and disorders
Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
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5 product images
Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
Interpolated concentrations of native beta-2 microglobulin in human serum, plasma, and urine samples.
The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:200, plasma (EDTA) 1:400, plasma (heparin) 1:200, and 1:400 urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1037.2 ng/mL in serum, 657.5 ng/mL in plasma (citrate), 1039.9 ng/mL in plasma (EDTA), 787.5 ng/mL in plasma (heparin), and 1348.2 ng/mL in urine.
Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
Interpolated concentrations of native beta-2 microglobulin in human HepG2 cell culture supernatant sample.
The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 1:32. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 119.4 ng/mL in HepG2 cell culture supernatant.
Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1093.9 ng/mL with a range of 727.2 – 1537.0 ng/mL.
Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
Example of human beta-2 microglobulin standard curve in Sample Diluent NS.
The beta-2 microglobulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
Human beta-2-microglobulin standard curve comparison.
Standard Curve comparison between the original Human beta-2-microglobulin SimpleStep ELISA kit and current Human beta-2-microglobulin SimpleStep ELISA kit.
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