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AB285328

Human Beta catenin ELISA Kit

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Human Beta catenin ELISA Kit is a Sandwich ELISA for the measurement of Human Beta catenin in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

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Sandwich ELISA - Human Beta catenin ELISA Kit (AB285328)
  • sELISA

Supplier Data

Sandwich ELISA - Human Beta catenin ELISA Kit (AB285328)

β-catenin (human) ELISA Kit Standard Curve.

Standard Curve : This standard curve is for demonstration only. A standard curve must be run with each assay

Key facts

Detection method

Colorimetric

Sample types

Tissue, Cell culture media, Serum, Other biological fluids, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Range

0.156 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Beta catenin ELISA Kit (ab285328, K3381) is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human β-catenin captured onto the plate.

This ELISA kit shows no species cross-reactivity.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285328-96Test", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Sample Diluent", "number":"AB285328-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Chromogen Solution A", "number":"AB285328-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Chromogen Solution B", "number":"AB285328-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"HRP-Conjugate Reagent", "number":"AB285328-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB285328-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"30X Wash Solution", "number":"AB285328-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Sealed Bag", "number":"AB285328-CMP11", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plate cover", "number":"AB285328-CMP07", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Standard Diluent", "number":"AB285328-CMP06", "productcode":"" }, { "size":"1 x 500 µL", "name":"Human beta-catenin Standard (18 ng/ml)", "number":"AB285328-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human beta-catenin antibody pre-coated Microplate", "number":"AB285328-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
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Product protocols

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Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Also acts as a coactivator for other transcription factors, such as NR5A2 (PubMed : 22187462). Promotes epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via driving transcription of CTNNB1/TCF-target genes (PubMed : 29910125). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).
See full target information CTNNB1
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