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AB309427

Human beta-Glucuronidase ELISA Kit

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Human beta-Glucuronidase ELISA Kit is a Sandwich ELISA for the measurement of Human beta-Glucuronidase in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Beta-glucuronidase, Beta-G1, GUSB

1 Images
Sandwich ELISA - Human beta-Glucuronidase ELISA Kit (AB309427)
  • sELISA

Supplier Data

Sandwich ELISA - Human beta-Glucuronidase ELISA Kit (AB309427)

Example of standard curve using ab309427. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.18 ng/mL

Range

0.18 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB166904

Anti-beta glucuronidase (GUSB) antibody [EPR10616]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (AB166904)

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Proteins & Peptides

AB219458

Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus)

Bioactive

SDS-PAGE - Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus) (AB219458)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human beta-Glucuronidase ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human beta-Glucuronidase in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human beta-Glucuronidase coated on a 96-well plate
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Recovery

[ { "sample": "Serum", "range": "104 - 129 %", "average": "= 111.7" }, { "sample": "Plasma", "range": "104 - 132 %", "average": "= 118.5" }, { "sample": "Cell culture media", "range": "80 - 125 %", "average": "= 108.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309427-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309427-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309427-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human beta-Glucuronidase/GUSB Protein Standard", "number":"AB309427-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin", "number":"AB309427-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human beta-Glucuronidase/GUSB Antibody-coated ELISA Plate", "number":"AB309427-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309427-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309427-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human beta-Glucuronidase/GUSB Detection Antibody", "number":"AB309427-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta glucuronidase also known as GUSB is an enzyme with several well-known names including b glucuronidase b-glucuronidase and beta-glucuronidase. It is a hydrolase enzyme with a molecular mass around 75 kDa. The enzyme is widely expressed in lysosomes of various tissues such as liver spleen and kidney. Mechanically beta-glucuronidase cleaves beta-D-glucuronic acid residues from glycosaminoglycans steroids and hormones an essential step for the degradation of glycosaminoglycans.
Biological function summary

Beta glucuronidase performs significant roles in cellular metabolism and molecule recycling. This enzyme aids in the breakdown and recycling of complex carbohydrates. By facilitating hydrolysis it helps in maintaining cellular homeostasis. Beta glucuronidase is not part of a complex; it functions independently within lysosomes. Its activity is vital for recycling endocytosed material and managing cellular turnover.

Pathways

Beta glucuronidase plays an important role in the lysosomal degradation pathway. This enzyme aids in glycosaminoglycan catabolism a process important for cellular waste disposal and recycling. Its activity connects with several proteins in the pathway notably those involved in lysosomal function such as Hexosaminidase. Beta glucuronidase's enzymatic function further integrates into the broader context of carbohydrate metabolism pathways enabling efficient breakdown of large carbohydrate molecules.

Mutations or deficiencies in beta glucuronidase lead to mucopolysaccharidosis type VII also called Sly syndrome. This lysosomal storage disorder results in the accumulation of undegraded glycosaminoglycans causing developmental delay skeletal abnormalities and other systemic issues. Additionally beta glucuronidase contributes to cancer biology where altered expressions can influence tumor progression. Proteins like glucocerebrosidase may interact indirectly impacting the disease's pathophysiology.
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Product protocols

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Target data

Plays an important role in the degradation of dermatan and keratan sulfates.
See full target information GUSB
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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