AB220651

Human Beta IG-H3 ELISA Kit

Recombinant
SimpleStep
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Human Beta IG-H3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Beta IG-H3 with a sensitivity of 6.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BIGH3, TGFBI, Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP

6 Images

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

sELISA

Supplier Data

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta IG-H3 concentration was determined to be 1,699.81 ng/mL with a range of 1,014.25 – 3,195.39 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Interpolated concentrations of native beta IG-H3 in human serum and plasma samples.

The concentrations of beta IG-H3 were measured in duplicates, interpolated from the beta IG-H3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 4,000, plasma (EDTA) 1 : 4,000, and plasma (heparin) 1 : 4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta IG-H3 concentration was determined to be 1,371.41 ng/mL in neat serum, 1,229.89 ng/mL in neat plasma (citrate), 1,480.27 ng/mL in neat plasma (EDTA), and 1,470.88 ng/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Interpolated concentrations of native beta IG-H3 in human U87-MG cell extract based on a 10 μg/mL extract load.

The concentrations of beta IG-H3 were measured in duplicate and interpolated from the beta IG-H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta IG-H3 concentration was determined to be 745.58 pg/mL in U87-MG cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Interpolated concentrations of native beta IG-H3 in human HUVEC supernatant.

The concentrations of beta IG-H3 were measured in duplicates, interpolated from the beta IG-H3 standard curves and corrected for sample dilution. Undiluted samples are as follows : HUVEC supernatant 8%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta IG-H3 concentration was determined to be 18,714.03 pg/mL in neat HUVEC supernatant. HUVEC supernatants were cultured in RPMI base media with 10% fetal bovine serum.

sELISA

Supplier Data

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Example of human beta IG-H3 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Example of human beta IG-H3 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.3 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Beta IG-H3 ELISA Kit (ab220651) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta IG-H3 protein in cell culture extracts, cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Beta IG-H3 with 6.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human beta IG-H3 is also known as Kerato-epithelin and RGD-CAP (RGD-containing collagen associated protein), is a secreted adhesion molecule that binds to collagens and integrins via covalent and noncovalent interactions. It encodes a 683-amino acid protein containing a secretory signal sequence and four homologous internal domains. Mouse and bovine beta IG-H3 proteins are 90.6% and 92.7% homologous to human beta IG-H3, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 107 %", "average": "= 104" }, { "sample": "Serum", "range": "97 - 101 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "98 - 105 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "92 - 98 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "91 - 99 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "97 - 104 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "84 - 90 %", "average": "= 88" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab220651-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB220651-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB220651-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB220651-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human beta IG-H3 Lyophilized Recombinant Protein", "number":"AB220651-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB220651-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human beta IG-H3 Capture Antibody", "number":"AB220651-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human beta IG-H3 Detector Antibody", "number":"AB220651-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB220651-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB220651-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB220651-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB220651-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB220651-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Plays a role in cell adhesion (PubMed : 8024701). May play a role in cell-collagen interactions (By similarity).

See full target information TGFBI

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Beta IG-H3 ELISA Kit

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Sandwich ELISA - Human Beta IG-H3 ELISA Kit (AB220651)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise