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AB155426

Human beta IG H3 ELISA Kit (TGFBI)

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(6 Publications)

Human beta IG H3 ELISA Kit (TGFBI) is a Sandwich (quantitative) ELISA for the measurement of Human beta IG H3 (TGFBI) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

BIGH3, TGFBI, Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP

2 Images
Sandwich ELISA - Human beta IG H3 ELISA Kit (TGFBI) (AB155426)
  • sELISA

Unknown

Sandwich ELISA - Human beta IG H3 ELISA Kit (TGFBI) (AB155426)

Typical Standard Curve

Sandwich ELISA - Human beta IG H3 ELISA Kit (TGFBI) (AB155426)
  • sELISA

Unknown

Sandwich ELISA - Human beta IG H3 ELISA Kit (TGFBI) (AB155426)

Representative standard curve using ab155426 - Assay Diluent A.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 25 pg/mL

Range

0.025 - 6 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's beta IG H3 (TGFBI) Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human beta IG H3 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human beta IG H3 (TGFBI) coated on a 96-well plate. Standards and samples are pipetted into the wells and beta IG H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta IG H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta IG H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization maybe required with urine samples.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "76 - 113 %", "average": "= 92.44" }, { "sample": "Plasma", "range": "69 - 104 %", "average": "= 86.91" }, { "sample": "Cell culture media", "range": "96 - 114 %", "average": "= 104.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155426-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155426-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155426-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"beta IG H3 Microplate (12 x 8 wells)", "number":"AB155426-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB155426-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human beta IG H3", "number":"AB155426-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB155426-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human beta IG H3 Standard (lyophilized)", "number":"AB155426-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155426-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155426-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Plays a role in cell adhesion (PubMed : 8024701). May play a role in cell-collagen interactions (By similarity).
See full target information TGFBI
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 17:50 PubMed38395907

2024

Novel prognostic marker TGFBI affects the migration and invasion function of ovarian cancer cells and activates the integrin αvβ3-PI3K-Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Yin-Hai Xu,Yi Guo

Theranostics 12:4221-4236 PubMed35673564

2022

TGFBI secreted by tumor-associated macrophages promotes glioblastoma stem cell-driven tumor growth via integrin αvβ5-Src-Stat3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Peng,Hongtao Zhu,Dan Liu,Zirong Chen,Xiaolin Zhang,Zhongyin Guo,Minhai Dong,Lijun Wan,Po Zhang,Guohao Liu,Suojun Zhang,Fangyong Dong,Feng Hu,Fangling Cheng,Shijun Huang,Dongsheng Guo,Bin Zhang,Xingjiang Yu,Feng Wan

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33431665

2021

The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Ji Yong Jang,Mehdi Pirooznia,Shihui Liu,Toren Finkel

Oncogenesis 7:40 PubMed29795279

2018

Dysregulation of the MiR-449b target TGFBI alters the TGFβ pathway to induce cisplatin resistance in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre-Antoine Bissey,Jacqueline H Law,Jeff P Bruce,Wei Shi,Aline Renoult,Melvin L K Chua,Kenneth W Yip,Fei-Fei Liu

Neoplasia (New York, N.Y.) 20:32-43 PubMed29190493

2017

BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhong Pan,Song-Chang Lin,Kai-Jie Yu,Guoyu Yu,Jian H Song,Valerae O Lewis,Justin E Bird,Bryan Moon,Patrick P Lin,Nizar M Tannir,Eric Jonasch,Christopher G Wood,Gary E Gallick,Li-Yuan Yu-Lee,Sue-Hwa Lin,Robert L Satcher

Stem cells international 2017:4206187 PubMed28894469

2017

Ex Vivo Expansion of Human Limbal Epithelial Cells Using Human Placenta-Derived and Umbilical Cord-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Min Nam,Yong-Sun Maeng,Eung Kweon Kim,Kyoung Yul Seo,Helen Lew
View all publications
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websiteProtocolBooklet
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