Human beta Nerve Growth Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human beta Nerve Growth Factor with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations
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- Frontiers in cell and developmental biology 11:13282612024Human placenta-derived mesenchymal stem cells stimulate neuronal regeneration by promoting axon growth and restoring neuronal activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElvira H de Laorden,Diana Simón,Santiago Milla,María Portela-Lomba,Marian Mellén,Javier Sierra,Pedro de la Villa,María Teresa Moreno-Flores,Maite IglesiasPubMed 38188022
- Bioengineering (Basel, Switzerland) 9:2022Formation of Three-Dimensional Spheres Enhances the Neurogenic Potential of Stem Cells from Apical Papilla.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed S Basabrain,Jialin Zhong,Haiyun Luo,Junqing Liu,Baicheng Yi,Ahmed Zaeneldin,Junhao Koh,Ting Zou,Chengfei ZhangPubMed 36354515
- International journal of molecular sciences 23:2022Changes of Subjective Symptoms and Tear Film Biomarkers following Femto-LASIK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Tsai,Mohannad Alwees,Anika Rost,Janine Theile,H Burkhard Dick,Stephanie C Joachim,Suphi TaneriPubMed 35886858
- Scientific reports 11:201932021The surgical effect on overactive bladder symptoms in women with pelvic organ prolapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing-Ying Wu,Kuan-Hui Huang,Tsai-Hwa Yang,Hui-Shan Huang,Tzu-Shu Wang,Kuo-Chung Lan,Fei-Chi ChuangPubMed 34642384
- Biomaterials 275:1209242021Optimized design of a hyperflexible sieve electrode to enhance neurovascular regeneration for a peripheral neural interface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAustin Veith,Xue Li,Hailey Modi,Ali Abbaspour,Lan Luan,Chong Xie,Aaron B BakerPubMed 34147716
- Scientific reports 10:33752020Topical recombinant human Nerve growth factor (rh-NGF) is neuroprotective to retinal ganglion cells by targeting secondary degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Guo,Benjamin M Davis,Nivedita Ravindran,Joana Galvao,Neel Kapoor,Nasrin Haamedi,Ehtesham Shamsher,Vy Luong,Elena Fico,M Francesca CordeiroPubMed 32099056
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 27.3 - 1750 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
Alternative names
NGFB, NGF, Beta-nerve growth factor, Beta-NGF
What's included?
Recommended products
Human beta Nerve Growth Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human beta Nerve Growth Factor with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 27.3 - 1750 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 3.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 6.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 96 | Range 95 - 99 % |
Sample type Serum | Average % = 94 | Range 92 - 95 % |
Sample type EDTA Plasma | Average % = 88 | Range 83 - 92 % |
Sample type Urine | Average % = 96 | Range 94 - 100 % |
Sample type Heparin Plasma | Average % = 93 | Range 92 - 95 % |
Sample type Citrate plasma | Average % = 82 | Range 76 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human beta Nerve Growth Factor ELISA Kit (ab193760) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of beta Nerve Growth Factor protein in edta plasma, hep plasma, urine, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human beta Nerve Growth Factor with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Beta Nerve Growth Factor is involved in the development of maintenance of the sympathetic and sensory nervous systems. As a ligand for NTRK1 and NGFR receptors, it's involved in cell signaling cascades that regulate neuronal proliferation, differentiation, and survival.
Beta Nerve Growth Factor is also involved in modulation of the immune system and in the trauma response, and has been implicated in a variety of responses including memory and mental health.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta nerve growth factor (beta-NGF) is a significant protein that functions in the development and maintenance of the nervous system. It is also known as NGF or NGFB and has a molecular mass of around 26 kDa. This protein is highly expressed in a wide range of tissues including the brain skin and various glands. Researchers often use assays like growth factor assays and NGF ELISA kits to measure beta-NGF levels given its importance in several physiological processes.
- Biological function summary
Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.
- Pathways
Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.
- Associated diseases and disorders
Beta nerve growth factor has been connected to neurodegenerative diseases like Alzheimer's disease and chronic pain conditions. Alterations in beta-NGF expression or signaling can impact neuronal survival contributing to the progression of these disorders. In Alzheimer's disease for example changes in NGF interactions with proteins like amyloid-beta and tau may exacerbate neurodegeneration. Similarly in pain syndromes disruptions in beta-NGF signaling can influence pain perception through pathways involving other neurotrophic factors.
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4 product images
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (ab193760)
Example of beta Nerve Growth Factor standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (ab193760)
Example of beta Nerve Growth Factor standard curve in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (ab193760)
Example of beta Nerve Growth Factor standard curve in Sample Diluent 50BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (ab193760)
Titration of Human serum (spiked with 875 pg/mL purified beta Nerve Growth Factor) within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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