Human beta Nerve Growth Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human beta Nerve Growth Factor with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations
View Alternative Names
NGFB, NGF, Beta-nerve growth factor, Beta-NGF
- ELISA
Supplier Data
ELISA - Human beta Nerve Growth Factor ELISA Kit (AB193760)
- sELISA
Supplier Data
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (AB193760)
Example of beta Nerve Growth Factor standard curve in Sample Diluent 50BS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (AB193760)
Example of beta Nerve Growth Factor standard curve in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (AB193760)
Example of beta Nerve Growth Factor standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit (AB193760)
Titration of Human serum (spiked with 875 pg/mL purified beta Nerve Growth Factor) within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Reactivity data
Product details
Human beta Nerve Growth Factor ELISA Kit ab193760 is a rapid single-wash 90-min Sandwich ELISA to measure Human beta Nerve Growth Factor in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 6 pg/mL.
How the assay works
Human beta Nerve Growth Factor SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human beta Nerve Growth Factor ELISA Kit ab193760 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Beta Nerve Growth Factor is involved in the development of maintenance of the sympathetic and sensory nervous systems. As a ligand for NTRK1 and NGFR receptors, it's involved in cell signaling cascades that regulate neuronal proliferation, differentiation, and survival.
Beta Nerve Growth Factor is also involved in modulation of the immune system and in the trauma response, and has been implicated in a variety of responses including memory and mental health.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.
Pathways
Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (7)
Recent publications for all applications. Explore the full list and refine your search
Materials today. Bio 31:101491 PubMed39896288
2025
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in cell and developmental biology 11:1328261 PubMed38188022
2024
Applications
Unspecified application
Species
Unspecified reactive species
Bioengineering (Basel, Switzerland) 9: PubMed36354515
2022
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 23: PubMed35886858
2022
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 11:20193 PubMed34642384
2021
Applications
Unspecified application
Species
Unspecified reactive species
Biomaterials 275:120924 PubMed34147716
2021
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 10:3375 PubMed32099056
2020
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com