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AB326312

Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent

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Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human beta Nerve Growth Factor with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 4.8 - 3500 pg/ml with a sensitivity of 1.2pg/mL.

-Patent pending

View Alternative Names

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

4 Images
Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)
  • sELISA

Lab

Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)
  • sELISA

Lab

Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)

Dynamic range comparison between the Chemiluminescent Human beta Nerve Growth Factor SimpleStep ELISA, colorimetric Human beta Nerve Growth Factor SimpleStep ELISA (ab193760), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)
  • sELISA

Lab

Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)

Standard curve comparison between the Chemiluminescent Human beta Nerve Growth Factor SimpleStep ELISA and colorimetric Human beta Nerve Growth Factor SimpleStep ELISA (ab193760) in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)
  • sELISA

Supplier Data

Sandwich ELISA - Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (AB326312)

Titration of Human serum (spiked with 875 pg/mL purified beta Nerve Growth Factor) within the working range of the assay.

Background-subtracted data values (mean +/- SD n = 2) are graphed.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.2 pg/mL

Range

4.8 - 3500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (ab326312) is a rapid single-wash sandwich 55-min ELISA to measure Human beta Nerve Growth Factor in Cell culture supernatant, Serum, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma with a sensitivity of 1.2 pg/mL.

How the assay works

Human beta Nerve Growth Factor ELISA Kit, Chemiluminescent (ab326312) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human beta Nerve Growth Factor (ab326312) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 99 %", "average": "= 96" }, { "sample": "Serum", "range": "92 - 95 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "83 - 92 %", "average": "= 88" }, { "sample": "Urine", "range": "94 - 100 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "92 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "76 - 88 %", "average": "= 82" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326312-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human beta Nerve Growth Factor Detector Antibody", "number":"AB326312-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326312-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB326312-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human beta Nerve Growth Factor Capture Antibody", "number":"AB326312-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326312-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326312-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326312-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326312-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326312-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326312-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human beta Nerve Growth Factor Lyophilized Recombinant Protein", "number":"AB326312-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
See full target information NGF
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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