AB99986

Human Beta Nerve Growth Factor ELISA Kit (NGFB)

Name: Human Beta Nerve Growth Factor ELISA Kit (NGFB) (ab99986) | Abcam
Brand: Abcam Limited
SKU: AB99986
Price: 780 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(13 Publications)

Human Beta Nerve Growth Factor ELISA Kit (NGFB) is a Sandwich (quantitative) ELISA for the measurement of Human Beta Nerve Growth Factor (NGFB) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

1 Images

Sandwich ELISA - Human Beta Nerve Growth Factor ELISA Kit (NGFB) (AB99986)

sELISA

Supplier Data

Sandwich ELISA - Human Beta Nerve Growth Factor ELISA Kit (NGFB) (AB99986)

Representative Standard Curve using ab99986.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 14 pg/mL

Range

6.86 - 5000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA^® range.

NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "90 - 122 %", "average": "= 106.7" }, { "sample": "Serum", "range": "98 - 116 %", "average": "= 113.1" }, { "sample": "Plasma", "range": "85 - 117 %", "average": "= 102.8" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99986-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99986-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99986-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Beta Nerve Growth Factor Microplate (12 x 8 wells)", "number":"AB99986-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99986-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Beta Nerve Growth Factor Standard Human (recombinant) (lyophilized)", "number":"AB99986-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB99986-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Beta Nerve Growth Factor", "number":"AB99986-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB99986-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta nerve growth factor (beta-NGF) is a significant protein that functions in the development and maintenance of the nervous system. It is also known as NGF or NGFB and has a molecular mass of around 26 kDa. This protein is highly expressed in a wide range of tissues including the brain skin and various glands. Researchers often use assays like growth factor assays and NGF ELISA kits to measure beta-NGF levels given its importance in several physiological processes.

Biological function summary

Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.

Pathways

Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.

Beta nerve growth factor has been connected to neurodegenerative diseases like Alzheimer's disease and chronic pain conditions. Alterations in beta-NGF expression or signaling can impact neuronal survival contributing to the progression of these disorders. In Alzheimer's disease for example changes in NGF interactions with proteins like amyloid-beta and tau may exacerbate neurodegeneration. Similarly in pain syndromes disruptions in beta-NGF signaling can influence pain perception through pathways involving other neurotrophic factors.

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Product protocols

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Target data

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

See full target information NGF

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 48:1698-1713 PubMed39080995

2024

Overexpression of EGFL7 promotes angiogenesis and nerve regeneration in peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zengtao Hao,Zhiqi Huo,Qicheng Aixin-Jueluo,Tao Wu,Yihong Chen

Frontiers in immunology 13:783076 PubMed35300334

2022

Localization of Nerve Growth Factor Expression to Structurally Damaged Cartilaginous Tissues in Human Lumbar Facet Joint Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias F Seidel,Cordula Netzer,Véronique Chobaz,Thomas Hügle,Jeroen Geurts

Cell transplantation 30:9636897211035409 PubMed34318707

2021

Stamina-Enhancing Effects of Human Adipose-Derived Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jung Yoon,Hye Rim Seong,Jangbeen Kyung,Dajeong Kim,Sangryong Park,Ehn-Kyoung Choi,Yun-Bae Kim,Dongsun Park

Cancer reports (Hoboken, N.J.) 4:e1300 PubMed33026185

2020

Localization and hypersecretion of nerve growth factor in breast phyllodes tumors: Evidence from a preliminary study.

Applications

Unspecified application

Species

Unspecified reactive species

Ashutosh Kumar,Khursheed Raza,Tapas C Nag,Anurag Srivastava,Ritu Sehgal

Spanish journal of psychiatry and mental health 16:137-142 PubMed32674992

2020

Acute effects of a session of electroconvulsive therapy on brain-derived neurotrophic factor plasma levels.

Applications

Unspecified application

Species

Unspecified reactive species

Miquel Bioque,Karina S Mac-Dowell,Cristina Font,Ana Meseguer,Elisabet Macau,Marta Garcia-Orellana,Marc Valentí,Juan C Leza,Miquel Bernardo

Stem cells (Dayton, Ohio) 36:844-855 PubMed29476565

2018

Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Ping Cen,Tsz Kin Ng,Jia-Jian Liang,Xi Zhuang,Xiaowu Yao,Gary Hin-Fai Yam,Haoyu Chen,Herman S Cheung,Mingzhi Zhang,Chi Pui Pang

Molecular oncology 11:1189-1207 PubMed28557340

2017

TrkA is a binding partner of NPM-ALK that promotes the survival of ALK T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Shi,Suraj Konnath George,Bhawana George,Choladda V Curry,Albina Murzabdillaeva,Serhan Alkan,Hesham M Amin

Scientific reports 6:37360 PubMed27853274

2016

Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target.

Applications

ELISA

Species

Human

Hamish T J Gilbert,Nathan Hodson,Pauline Baird,Stephen M Richardson,Judith A Hoyland

Reproductive biology and endocrinology : RB&E 14:29 PubMed27233989

2016

Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Saeednia,Maryam Shabani Nashtaei,Hossein Bahadoran,Ashraf Aleyasin,Fardin Amidi

Scientific reports 5:16953 PubMed26586020

2015

Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis.

Applications

ELISA

Species

Unspecified reactive species

Christine V Fontanilla,Huiying Gu,Qingpeng Liu,Timothy Z Zhu,Changwei Zhou,Brian H Johnstone,Keith L March,Robert M Pascuzzi,Martin R Farlow,Yansheng Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Beta Nerve Growth Factor ELISA Kit (NGFB)

Sandwich ELISA - Human Beta Nerve Growth Factor ELISA Kit (NGFB) (AB99986)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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