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AB315055

Human beta Synuclein ELISA Kit - Intracellular

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Human beta Synuclein ELISA Kit - Intracellular is a Sandwich ELISA for the measurement of Human beta Synuclein - Intracellular in Human in Cell/Tissue Extracts samples.

View Alternative Names

Beta-synuclein, SNCB

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Sandwich ELISA - Human beta Synuclein ELISA Kit - Intracellular (AB315055)
  • sELISA

Supplier Data

Sandwich ELISA - Human beta Synuclein ELISA Kit - Intracellular (AB315055)

Interpolated concentration of native beta Synuclein was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human brain stem tissue extract 30 µg/mL, mouse brain tissue extract 16 µg/mL and rat brain tissue extract 8 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human beta Synuclein ELISA Kit - Intracellular (AB315055)
  • sELISA

Supplier Data

Sandwich ELISA - Human beta Synuclein ELISA Kit - Intracellular (AB315055)

Example of human beta Synuclein standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 24 pg/mL

Range

175 - 11200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB221908

Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (AB221908)

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Proteins & Peptides

AB140415

Recombinant Human beta Synuclein protein

SDS-PAGE - Recombinant Human beta Synuclein protein (AB140415)

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Assay Kits

AB315056

Human beta Synuclein ELISA Kit - Extracellular

SimpleStep

Sandwich ELISA - Human beta Synuclein ELISA Kit - Extracellular (AB315056)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human beta Synuclein ELISA kit is a sandwich ELISA designed for the quantitative measurement of beta Synuclein protein in Tissue Extracts. Quantitate Human beta Synuclein with 24 pg/ml sensitivity. The recombinant antibodies used in this kit guarantee specificity and consistency of results.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.66" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "94 - 96 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315055-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315055-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315055-CMP10", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315055-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315055-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS", "number":"AB315055-CMP07", "productcode":"" }, { "size":"3 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315055-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human beta Synuclein Capture Antibody", "number":"AB315055-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human beta Synuclein Lyophilized Recombinant Protein", "number":"AB315055-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB315055-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human beta Synuclein Detector Antibody", "number":"AB315055-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315055-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-synuclein also known as β-synuclein or SNCB is a protein that plays a role in the nervous system. It is a small protein with a molecular weight around 14 kDa. Predominantly beta-synuclein is found in presynaptic terminals of neurons in the central nervous system including the brain and spinal cord. This protein shares significant amino acid sequence identity with alpha-synuclein and gamma-synuclein comprising the synuclein family. It acts as a modulator of neuronal and synaptic function influencing the dynamics of neurotransmitter release.
Biological function summary

Beta-synuclein contributes to cellular homeostasis and synaptic integrity. It is not typically found as part of a larger protein complex like its relative alpha-synuclein which forms toxic aggregates. Instead beta-synuclein exhibits neuroprotective properties helping maintain neuronal health. It possibly regulates synaptic vesicle cycling and mitigates levels of alpha-synuclein aggregation. Research suggests that loss of beta-synuclein function might disrupt synaptic stability leading to neurological issues.

Pathways

Beta-synuclein engages in synaptic function and plasticity. It participates in pathways related to neurotransmitter release and synaptic vesicle trafficking. Beta-synuclein is connected to calcium/calmodulin-dependent protein kinase II (CaMKII) pathways. This interaction supports beta-synuclein's regulatory impact on synaptic vesicle dynamics negatively modulating the aggregation-prone behaviors seen in alpha-synuclein during synucleinopathies.

Beta-synuclein plays a role in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Research highlights its relationship with these conditions due to its interaction with alpha-synuclein known to form pathological aggregates and contribute to disease progression. Beta-synuclein's modulation of alpha-synuclein activity can impact the development and progression of these disorders. There is ongoing interest in its potential protective role suggesting it might be leveraged in therapeutic strategies against synucleinopathy-related diseases.
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Product protocols

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Target data

Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
See full target information SNCB
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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