AB245722

Human Beta-tubulin ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human Beta-tubulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Beta-tubulin with a sensitivity of 25.77 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TUBB4, TUBB5, TUBB4A, Tubulin beta-4A chain, Tubulin 5 beta, Tubulin beta-4 chain

2 Images

Sandwich ELISA - Human Beta-tubulin ELISA Kit (AB245722)

sELISA

Supplier Data

Sandwich ELISA - Human Beta-tubulin ELISA Kit (AB245722)

Interpolated concentrations of native Beta-Tubulin in human HeLa and 293 cell extract samples based on a 2 μg/mL extract load and Jurkat cell extract samples based on a 1 μg/mL extract load. The concentrations of Beta-Tubulin were measured in duplicate and interpolated from the Beta-Tubulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-Tubulin concentration was determined to be 8,281.0 pg/mL in HeLa, 3,557.9 pg/ml in Jurkat, and 6,718.6 pg/ml in 293 cell extract samples at the specified extract loads.

sELISA

Supplier Data

Sandwich ELISA - Human Beta-tubulin ELISA Kit (AB245722)

Example of human Beta-Tubulin standard curve in 1X Cell Extraction Buffer PTR. The Beta-Tubulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 25.77 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Beta-tubulin ELISA Kit (ab245722) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta-tubulin protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Beta-tubulin with 25.77 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "92 - 103 %", "average": "= 98" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245722-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245722-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245722-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB245722-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245722-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245722-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245722-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB245722-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Beta-tubulin Detector Antibody", "number":"AB245722-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245722-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Beta-tubulin Capture Antibody", "number":"AB245722-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Beta-tubulin Lyophilized recombinant Protein", "number":"AB245722-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB4A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 37:2679-2701 PubMed36154552

2022

Design and synthesis of new trimethoxylphenyl-linked combretastatin analogues loaded on diamond nanoparticles as a panel for ameliorated solubility and antiproliferative activity.

Applications

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Species

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Islam Zaki,Amal M Y Moustafa,Botros Y Beshay,Reham E Masoud,Mohammed A I Elbastawesy,Mohammed A S Abourehab,Mohamed Y Zakaria

Molecules (Basel, Switzerland) 27: PubMed35745081

2022

Design, Synthesis and Cytotoxic Activity Evaluation of Newly Synthesized Amides-Based TMP Moiety as Potential Anticancer Agents over HepG2 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tarfah Al-Warhi,Adil Aldhahrani,Fayez Althobaiti,Eman Fayad,Ola A Abu Ali,Sarah Albogami,Ali H Abu Almaaty,Amgad I M Khedr,Syed Nasir Abbas Bukhari,Islam Zaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Beta-tubulin ELISA Kit

Sandwich ELISA - Human Beta-tubulin ELISA Kit (AB245722)

Sandwich ELISA - Human Beta-tubulin ELISA Kit (AB245722)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise